PPT-Gram Stain

Gram Stain Reaction The Gram stain reaction named after the Danish bacteriologist Hans Christian Gram was originally devised in 1882 Gram staining is based on the ability of the bacterial cell wall to retain the crystal violet dye . Staining total protein before specific protein de tection techniques provides an assessment of protein transfer efficiency and makes it possible to detect contaminating proteins in the sample and to compare the sample with molecular weight standards Cheng . Zhang. Thurs 1 Dec 11. MM Tutorial. Gram staining. Fixed film (heat kill bacteria etc...). Methyl violet . Lugol’s. /gram’s iodine. Decolourise with acetone. Methyl red .  . STAIN 2 (counter stain): pink-red colour. Clinical Bacteriology II. CLS 413. Mrs. . Deemah. . Dabbagh. CLS Department. College of Applied Medical Sciences. King Saud University. Course Content. Enterobacteriacae. Lactose Fermenters:. Escherichia coli. A stain is a substance that adheres to a cell, giving the cell color. .. The presence of color gives the cells significant contrast so are much more visible. . Different stains have different affinities for different organisms, or different parts of . Cytochemical Reactions in Acute Leukemia. Blasts Identified. Cellular Element Stained. Cytochemical Reaction. Myeloblasts strong positive; monoblasts faint positive. Neutrophil primary granules. Myeloperoxidase (MPO). Goals and Objectives. :. Exercise . 37. : Morphological Study of Unknown Bacterium. Each person make . smears of assigned organism: one from plate, one from broth (review smears Exercise 11). Gram stain (Exercise 15) and record results: Gram reaction, . Gram stain . Ziehl. . Neelsen. stain. . Requires the use of at least 3 chemical reagents.. Primary Stain. : . stain all the cells.. Decolorizing agent: . may or may not remove the primary stain from the entire the cell or only from certain cell structure. . .. The presence of color gives the cells significant contrast so are much more visible. . Different stains have different affinities for different organisms, or different parts of . organisms. They are used to differentiate different types of organisms or to view specific parts of organisms. 1. What type of bloodstain?. 2. What type of blood stain?. 3. What type of blood stain?. 4. . . What type of blood stain?. 5. What type of blood stain?. 6. What type of blood stain?. 7. type of blood stain?. Only use oil on 100x objective lens. Never get it onto any of the other objectives! . Never use the coarse adjustment with the 40x and 100x objectives.. . Ex. . 3: Staining Techniques - Gram Stain, part 1. Differentiate bacteria into two large groups (the Gram Positive and the Gram negative).. Gram status is important in medicine; the presence or absence of a cell wall will change the bacterium's susceptibility to some antibiotics.. Faculty: Dr. Rakesh Sharda OBJECTIVES OF STAINING Improves visibiltiy by greater contrast between the organism and the background, differentiate various morphological types (by shape, size, arrangeme Lab # 6. NEISSERIA SPECIES. Contains many species; 2 are human pathogens. Neisseria . gonorrhoea. (gonococcus). Neisseria . meningitidis. (. meningococcus. ). Other species are normal flora that inhabit the upper respiratory tract of humans.. 5/29/2023. 1. Practical Bacteriology. lab. . 2. By:. Asst. Prof. Shiama'a Al-Salihy. Learning objectives:. 5/29/2023. 2. After this lab, you must be able to:. Identify laboratory diagnosis steps.. Understand staining techniques.