PPT-What is a Stain

A stain is a substance that adheres to a cell giving the cell color The presence of color gives the cells significant contrast so are much more visible Different stains have different affinities for different organisms or different parts of . بسم الله الرحمن الرحيم. Diagnostic Medical Microbiology-Laboratory Manual. An . etiological diagnosis of lower respiratory tract infection by microscopic examination and culture with identification and susceptibility test of . . sp. Basmah. . almaarik. #Lab . 9. Bacillus culture. Spore forming facultative anaerobic bacteria.. Grow every where cause it produce spores.. Grow in wide rage 12-45 ºC. Spore formation is best seen in 25 – 30 ºC. Gram Stain Reaction. The Gram stain reaction, named after the Danish bacteriologist Hans Christian Gram, was originally devised in 1882.. Gram staining is based on the ability of the bacterial cell wall to retain the crystal violet dye. . New . Product Introduction. Summer 2013. Previously Introduced. . Clear Interior Finish . All Marvin window and door products. Both wood and Clad. All wood species. Standard lead times. Water-borne Acrylic Enamel coating. A stain is a substance that adheres to a cell, giving the cell color. .. The presence of color gives the cells significant contrast so are much more visible. . Different stains have different affinities for different organisms, or different parts of . Gram stain . Ziehl. . Neelsen. stain. . Requires the use of at least 3 chemical reagents.. Primary Stain. : . stain all the cells.. Decolorizing agent: . may or may not remove the primary stain from the entire the cell or only from certain cell structure. . Spore stain. Capsule stain. Endospores. . are formed by some bacteria, aerobic . Bacillus. species and anaerobic . Clostridium. species.. Non active, resistant to heating and chemicals.. Remain dormant until favorable conditions return.. BIOLOGY & BIOTECHNOLODY DEPARTMENT. In our laboratory, . bacterial morphology . (form and structure) may be examined in two ways:. By observing . living unstained organisms. (. wet mount. ).. 2. By . Bacterial Taxonomy: How are these unicellular organisms classified?. complex system of classification. based on shape & size; oxygen, pH, and temperature requirements; laboratory characteristics, biochemical . Ms. Schultz. The Sam Sheppard Case. On July 4, 1954 Sam Sheppard called police to report that his wife Marilynn was dead in the bedroom. He said the night before, his wife had gone upstairs to bed while he was asleep on the couch. When he went upstairs, he saw his wife covered in blood. He heard a noise and ran downstairs chasing after a 6’3’’ bushy haired man. The two struggled and Dr. Sheppard was choked unconscious. When police arrived, Marilynn was found with 35 wounds to the head and blood drenched the walls, door, and bed. Her face was almost unrecognizable. Sheppard served 10 years in prison before the US Supreme court rules that the trial had been tainted. The evidence was reexamined. Blood spatters and blood drops provided some of the most compelling evidence. Dr. Paul Kirk, an expert in blood analysis, determined the killer could not have been Sam because the attack was perpetrated by a left handed person. Dr. Sheppard was right handed.. .. The presence of color gives the cells significant contrast so are much more visible. . Different stains have different affinities for different organisms, or different parts of . organisms. They are used to differentiate different types of organisms or to view specific parts of organisms. 1. What type of bloodstain?. 2. What type of blood stain?. 3. What type of blood stain?. 4. . . What type of blood stain?. 5. What type of blood stain?. 6. What type of blood stain?. 7. type of blood stain?. Differentiate bacteria into two large groups (the Gram Positive and the Gram negative).. Gram status is important in medicine; the presence or absence of a cell wall will change the bacterium's susceptibility to some antibiotics. refractile. body within the vegetative cells. Procedure: (fig.10) . 1- Prepare a fixed smear.. 2- Flood the smear with 5% malachite green and let the stain react for 1 minute .Using your . bunsen. burner, heat the top of the stained slide until steam rises but do not let it boil. This step is performed by passing the burner back and over the slide for about 5 minutes..