PPT-Laboratory diagnosis Smear preparation, Simple and Gram stains

5292023 1 Practical Bacteriology lab 2 By Asst Prof Shiamaa AlSalihy Learning objectives 5292023 2 After this lab you must be able to Identify laboratory diagnosis steps Understand staining techniques. A well Made and well Stained Smear can provide: . Estimates of cell count. Proportions of the different types of WBC. Morphology. Peripheral Blood Smear. Objective. 1. Specimen Collection. 2. Peripheral Smear Preparation. DIAGNOSIS OF . NEOPLASM. Depend on triple test. LABORATORY. . &. PATHOLOGY . data. IMAGING. CLINICAL. ROLE OF PATHOLOGY. PRE- . operative intra operative . POST . oprative. A stain is a substance that adheres to a cell, giving the cell color. .. The presence of color gives the cells significant contrast so are much more visible. . Different stains have different affinities for different organisms, or different parts of . T. E. R. I. A. L. . S. T. A. I. N. I. N. G. 1. What is a Stain. A stain is a substance that adheres to a cell, giving the cell color.. The presence of color gives the cells significant contrast so are much more visible. . BIOLOGY & BIOTECHNOLODY DEPARTMENT. In our laboratory, . bacterial morphology . (form and structure) may be examined in two ways:. By observing . living unstained organisms. (. wet mount. ).. 2. By . A stain is a substance that adheres to a cell, giving the cell color. .. The presence of color gives the cells significant contrast so are much more visible. . Different stains have different affinities for different organisms, or different parts of . .. The presence of color gives the cells significant contrast so are much more visible. . Different stains have different affinities for different organisms, or different parts of . organisms. They are used to differentiate different types of organisms or to view specific parts of organisms. Basic vs. acidic dyes. Differential Stain. Primary stain (stains all cells on slide). Decolorizing step (removes stain from certain types of cells). Counterstain (stains the decolorized cells). Introduction . Direct . fecal . smears. Direct . fecal smears can be used as a quick screening test to check for any intestinal . parasite.. Advantages . &Disadvantages . Advantages:-. U. seful . By. Assistant lecturer. Zainab farooq . shafeeq. Bacterial Morphology. Bacteria: are unicellular free living organisms without . chlorophil. having doth . DNAand. RNA . Bacterial . morphology. Can . tissues, cells, cell components, cell contents, or microorganisms.. *The stain may be . natural or synthetic. . . *The object stained is called the . substrate. . . *Staining facilitates the observation of a substrate by introducing . Faculty: Dr. Rakesh Sharda OBJECTIVES OF STAINING Improves visibiltiy by greater contrast between the organism and the background, differentiate various morphological types (by shape, size, arrangeme . Essam. Bacterial Morphology. Bacteria: are unicellular free living organisms without . chlorophil. having . b. oth DNA and RNA . Bacterial . morphology. Can . be grouped into three types . 1- Spherical . Bacterial Smear Preparation. It is a preparation that includes a . small amount of biological sample or culture . spread. . in a very thin film on the surface of the slide. . . Smear preparation is a step before staining..