PPT-Special Staining; spore and capsule

Lab 4 Medgar Evers College Bio 261 Microbiology Prof Santos Aim 1 exercise 14 Capsular staining Capsule extracellular layer composed of polysaccharides found in some bacteria that plays a role in immunity and adhesion. SPORE 79 PAGE 16 SPORE is a bi-monthly publication providing information on agricultural development for ACP countries Technical Centre for Agricultural and Rural Cooperation (CTA)CTA: Postbus 380, 67 HAEMATOXYLIN & EOSIN. . Supervised by : . Dr.Sherifa. . Prepared by: Reem Aldossari. Lama . Alabdi. H&E staining:. The best known and most widely used staining procedure, . hematoxylin. -eosin staining (H&E), uses . Spores . are resistant to heat and chemicals because of a tough outer covering made of the protein . keratin.. The keratin also resists staining, so extreme measures must be taken to stain the spore.. Spores . are resistant to heat and chemicals because of a tough outer covering made of the protein . keratin.. The keratin also resists staining, so extreme measures must be taken to stain the spore.. Special stain. The . spore stain . . (. Schaeffer-Fulton method) . Many species of bacteria can exist in . two . very different states. .. . In favorable environments. , they exist as metabolically active . Spore stain. Capsule stain. Endospores. . are formed by some bacteria, aerobic . Bacillus. species and anaerobic . Clostridium. species.. Non active, resistant to heating and chemicals.. Remain dormant until favorable conditions return.. Cytochemical Reactions in Acute Leukemia. Blasts Identified. Cellular Element Stained. Cytochemical Reaction. Myeloblasts strong positive; monoblasts faint positive. Neutrophil primary granules. Myeloperoxidase (MPO). Staining. Coloring organism with a dye. Microorganisms must be . FIXED. to microscopic slide first. Kills microbe. Attaches organism to the slide. Preserves various parts with minimal distortion. Steps to fix:. A.L. Noor Ameer . The reasons of staining. 1- To study their shapes . 2- . To differentiate the species of bacteria by using differential . stain.. 3- . To study the internal components of the bacterial . . Bacterial staining . Lab. 5 . A.L. Noor Ameer. Purpose of culturing is: . 1- Isolation of microorganisms from a . sample.. 2- . For counting the microorganism in the . sample.. 3- . Obtain pure . العملي. Lab -1. -2-. . Bacterial Taxonomy. Bacteria are classified and identified to distinguish among strains and to group them by criteria of interest to microbiologists and other scientists.. This. procedure is called . so,when. certain bacteria or some of their . structures could not be stained or seen or being differential with other bacteria cells when using the ordinary . staining,such. refractile. body within the vegetative cells. Procedure: (fig.10) . 1- Prepare a fixed smear.. 2- Flood the smear with 5% malachite green and let the stain react for 1 minute .Using your . bunsen. burner, heat the top of the stained slide until steam rises but do not let it boil. This step is performed by passing the burner back and over the slide for about 5 minutes.. Bacterial Smear Preparation. It is a preparation that includes a . small amount of biological sample or culture . spread. . in a very thin film on the surface of the slide. . . Smear preparation is a step before staining..