PPT-Endospore Staining

Spores are resistant to heat and chemicals because of a tough outer covering made of the protein keratin The keratin also resists staining so extreme measures must be taken to stain the spore. Neg a INTRODUCTION Negative staining is an easy, rapid, qualitative method for examining the structure of isolated organelles, individual macromolecules and viruses at the EM level. However, the meth HAEMATOXYLIN & EOSIN. . Supervised by : . Dr.Sherifa. . Prepared by: Reem Aldossari. Lama . Alabdi. H&E staining:. The best known and most widely used staining procedure, . hematoxylin. -eosin staining (H&E), uses . Spores . are resistant to heat and chemicals because of a tough outer covering made of the protein . keratin.. The keratin also resists staining, so extreme measures must be taken to stain the spore.. (BD Biosciences Protocol) Materials: Staining medium (SM) [1X HBSS; 2% (v/v) calf serum; 10mM NaN 3 10 mM HEPES, pH 7.2] Sterile filtered calf serum (CS) [0.45m TC sterile filtered] Gey’s solu Lab #4 . Medgar Evers College. Bio 261 Microbiology. Prof. Santos. Aim #1 exercise 14. Capsular staining. Capsule- extracellular layer composed of polysaccharides found in some bacteria that plays a role in immunity and adhesion.. Staining. Coloring organism with a dye. Microorganisms must be . FIXED. to microscopic slide first. Kills microbe. Attaches organism to the slide. Preserves various parts with minimal distortion. Steps to fix:. A.L. Noor Ameer . The reasons of staining. 1- To study their shapes . 2- . To differentiate the species of bacteria by using differential . stain.. 3- . To study the internal components of the bacterial . Ef31cientBest-in-Class Productivity149True walk-away solution designed for LEAN processes149Continuous loading and unloading evens out your workload149Unique rack design facilitating efficient workflo for paraffin embedded tissueinfoarigobiocomwwwarigobiocom1/4Materials and Reagentsused in this protocolDescriptionFormalin or other aldehyde fixativesEx Formaldehyde glutaraldehyde forms protein cross Faculty: Dr. Rakesh Sharda OBJECTIVES OF STAINING Improves visibiltiy by greater contrast between the organism and the background, differentiate various morphological types (by shape, size, arrangeme 4327 Abstract.OBJECTIVE: Erythropoietin (EPO), as a type of the tissue-protective cytokines, is a 30.4 kDa hematopoietic glycoprotein. The purpose of this study was to explore the neuroprotective effe This. procedure is called . so,when. certain bacteria or some of their . structures could not be stained or seen or being differential with other bacteria cells when using the ordinary . staining,such. ). Endospores form within the . Cell. 2. FE206 Food Microbiology I. Endospore. is . dormant stage of some bacterium that allows it to survive . unfavorable conditions . that would normally . be lethal such . Fixatives used in histopathology. 2. Staining. Differential staining. Gram’s staining. Vital staining. Trypan. blue- dead cells take up the stain . i.e. stain positively and live cells . Exclude the stain or stain negatively.