PPT-Bacterial staining Lab. 6

AL Noor Ameer The reasons of staining 1 To study their shapes 2 To differentiate the species of bacteria by using differential stain 3 To study the internal components of the bacterial . HAEMATOXYLIN & EOSIN. . Supervised by : . Dr.Sherifa. . Prepared by: Reem Aldossari. Lama . Alabdi. H&E staining:. The best known and most widely used staining procedure, . hematoxylin. -eosin staining (H&E), uses . (BD Biosciences Protocol) Materials: Staining medium (SM) [1X HBSS; 2% (v/v) calf serum; 10mM NaN 3 10 mM HEPES, pH 7.2] Sterile filtered calf serum (CS) [0.45m TC sterile filtered] Gey’s solu Staining. Coloring organism with a dye. Microorganisms must be . FIXED. to microscopic slide first. Kills microbe. Attaches organism to the slide. Preserves various parts with minimal distortion. Steps to fix:. . Bacterial staining . Lab. 5 . A.L. Noor Ameer. Purpose of culturing is: . 1- Isolation of microorganisms from a . sample.. 2- . For counting the microorganism in the . sample.. 3- . Obtain pure . & . Antibiotic susceptibility testing. : An introduction. Dr. Mohit Bhatia. Assistant Professor. Department of Microbiology. AIIMS, Rishikesh. Staining techniques . Smear preparation. Smear = 2 × 3 cm in size. . Bacteria: are unicellular free living organisms without . chlorophil. having . b. oth DNA and RNA . Bacterial . morphology. Can . be grouped into three types . 1- Spherical . (. cocci. ). 2- Rod – . By. Assistant lecturer. Zainab farooq . shafeeq. Bacterial Morphology. Bacteria: are unicellular free living organisms without . chlorophil. having doth . DNAand. RNA . Bacterial . morphology. Can . tissues, cells, cell components, cell contents, or microorganisms.. *The stain may be . natural or synthetic. . . *The object stained is called the . substrate. . . *Staining facilitates the observation of a substrate by introducing . العملي. Lab -1. -2-. . Bacterial Taxonomy. Bacteria are classified and identified to distinguish among strains and to group them by criteria of interest to microbiologists and other scientists.. Faculty: Dr. Rakesh Sharda OBJECTIVES OF STAINING Improves visibiltiy by greater contrast between the organism and the background, differentiate various morphological types (by shape, size, arrangeme . Essam. Bacterial Morphology. Bacteria: are unicellular free living organisms without . chlorophil. having . b. oth DNA and RNA . Bacterial . morphology. Can . be grouped into three types . 1- Spherical . This. procedure is called . so,when. certain bacteria or some of their . structures could not be stained or seen or being differential with other bacteria cells when using the ordinary . staining,such. Fixatives used in histopathology. 2. Staining. Differential staining. Gram’s staining. Vital staining. Trypan. blue- dead cells take up the stain . i.e. stain positively and live cells . Exclude the stain or stain negatively. Bacterial Smear Preparation. It is a preparation that includes a . small amount of biological sample or culture . spread. . in a very thin film on the surface of the slide. . . Smear preparation is a step before staining..