PPT-Medical microbiology Lab 3: The hanging drop slide and bacterial motility; Acid fast staining

Asst Lec Mariam A Ali Asst Lec Iman k kadhim AlMustaqbal University college 2 st Class 1 st Semester Department of pharmacy . Mel & Dave. (plus Poppy helped). Any errors found in this presentation are Mel’s fault, anything good thank Dave. GASTRIC ANATOMY. GASTRIC MOTILITY. Receptive Relaxation. Stomach is empty and contracted initially. Why It Should Be Important to You.  . James . Poupard. , Ph.D., F(AAM). Pharma Institute of Philadelphia .  . Center for the History of Microbiology/. ASM Archives (CHOMA).  . . 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Meet our renowned Organizing Committee Members: GIULIO TARRO Research Interest: Microbiology and Immunology Temple University Center for Biotechnology Italy MOHAMMAD MAHMOUD EFFAT Research Interest: Microbiology, Immunology and AIDs National Research Center Egypt SEGUNDO MESA CASTILLO Research Interest: Microbiology, Neurology Psychiatric Hospital of Havana Cuba AHMED GAFFER HEGAZI Research Interest: Immunology and Immunotherapy National Research Center Egypt Microbiology has turned out to be progressively vital to human culture. It has risen as a standout amongst the most imperative branches of life sciences. The consumables, equipment and innovation markets in the microbiology business totalled nearly $7.7 billion in 2012. This aggregate is expected to grow from $8.5 billion in 2013 to $11.4 billion in 2018, with a compound annual growth rate (CAGR) of 6.1% for the five-year time frame, 2013 to 2018. The global clinical microbiology market is anticipated to achieve USD 5.77 Billion by 2021 from USD 3.35 Billion in 2016, growing at a CAGR of 11.5% from 2016 to 2021. For updated information about conference, visit the conference website at https://www.meetingsint.com/conferences/appliedmicrobiology Target Audience Microbiology Organization Microbiologist, Professors, Scientists, Researchers, Doctors, Academicians, Business professionals, Government Officials, and young scientist Microbiology Conference 2019..!! We take great pleasure in inviting your esteemed self to “Meetings International Conference” (Microbiology 2019) scheduled during May 15-16, 2019 in the city Brussels, Belgium as a Speaker/Delegate/Exhibitor and share your experiences to enlighten esteemed personalities and researchers by attending the conference. Salient features: People from Industries, research institutes, academic universities, associations and societies will be attending. Your presence will definitely have positive impact on their future ventures and innovations. For more details: - https://www.meetingsint.com/conferences/appliedmicrobiology With regards Albert Robinson Program Manager | Applied Microbiology - 2019 Email: - robinsonalbert682@gmail.com, appliedmicrobiology@insightsummits.com Microbiology Conference, Microbiology Workshop, Microbiology Congress, Microbiology Summit, Microbiology Event, Microbiology Symposium, Applied Microbiology Conference Differentiate bacteria into two large groups (the Gram Positive and the Gram negative).. Gram status is important in medicine; the presence or absence of a cell wall will change the bacterium's susceptibility to some antibiotics. . Bacterial staining . Lab. 5 . A.L. Noor Ameer. Purpose of culturing is: . 1- Isolation of microorganisms from a . sample.. 2- . For counting the microorganism in the . sample.. 3- . Obtain pure . Leonard Weinstock, MD. Disclosures. Speakers Bureaus. Salix. Entera. Health. Forrest. Off label use of medications. . Low Dose Naltrexone: . Motility Effects. Unexpected effect on endorphins by being an opioid blocker. Bacteria: are unicellular free living organisms without . chlorophil. having . b. oth DNA and RNA . Bacterial . morphology. Can . be grouped into three types . 1- Spherical . (. cocci. ). 2- Rod – . By. Assistant lecturer. Zainab farooq . shafeeq. Bacterial Morphology. Bacteria: are unicellular free living organisms without . chlorophil. having doth . DNAand. RNA . Bacterial . morphology. Can . tissues, cells, cell components, cell contents, or microorganisms.. *The stain may be . natural or synthetic. . . *The object stained is called the . substrate. . . *Staining facilitates the observation of a substrate by introducing . Faculty: Dr. Rakesh Sharda OBJECTIVES OF STAINING Improves visibiltiy by greater contrast between the organism and the background, differentiate various morphological types (by shape, size, arrangeme refractile. body within the vegetative cells. Procedure: (fig.10) . 1- Prepare a fixed smear.. 2- Flood the smear with 5% malachite green and let the stain react for 1 minute .Using your . bunsen. burner, heat the top of the stained slide until steam rises but do not let it boil. This step is performed by passing the burner back and over the slide for about 5 minutes.. Fixatives used in histopathology. 2. Staining. Differential staining. Gram’s staining. Vital staining. Trypan. blue- dead cells take up the stain . i.e. stain positively and live cells . Exclude the stain or stain negatively. Bacterial Smear Preparation. It is a preparation that includes a . small amount of biological sample or culture . spread. . in a very thin film on the surface of the slide. . . Smear preparation is a step before staining..