PPT-Medical microbiology Lab 3: The hanging drop slide and bacterial motility; Acid fast staining

Asst Lec Mariam A Ali Asst Lec Iman k kadhim AlMustaqbal University college 2 st Class 1 st Semester Department of pharmacy . HANGING-DROP METHOD. USE OF SEMI-SOLID MEDIA. STAINING OF FLAGELLA. SEROLOGICAL TEST. FLUORESCENT TEST. SWARMING PHENOMENA. METHODS OF IDENTIFYNG MICROORGANISM. 2. MORPHOLOGY AND STAINING. 3 GENERAL WAYS. Motility is closely linked with chemotaxis, the ability to orientate along certain chemical gradients.. A large number of bacteria are motile. . . Most . possess one or more flagella on their surface that allow them to swim. . Motility is closely linked with chemotaxis, the ability to orientate along certain chemical gradients.. A large number of bacteria are motile. . . Most . possess one or more flagella on their surface that allow them to swim. . Key concepts:. . Wet mount technique. Motility. Bacterial . flagella and their arrangement . Cover glasses. Depression . slides. Petroleum. Jelly . (Vasoline). Toothpicks. Bacterial culture. Motility Determination:. A.L. Noor Ameer . The reasons of staining. 1- To study their shapes . 2- . To differentiate the species of bacteria by using differential . stain.. 3- . To study the internal components of the bacterial . Bacteria: are unicellular free living organisms without . chlorophil. having . b. oth DNA and RNA . Bacterial . morphology. Can . be grouped into three types . 1- Spherical . (. cocci. ). 2- Rod – . By. Assistant lecturer. Zainab farooq . shafeeq. Bacterial Morphology. Bacteria: are unicellular free living organisms without . chlorophil. having doth . DNAand. RNA . Bacterial . morphology. Can . tissues, cells, cell components, cell contents, or microorganisms.. *The stain may be . natural or synthetic. . . *The object stained is called the . substrate. . . *Staining facilitates the observation of a substrate by introducing . العملي. Lab -1. -2-. . Bacterial Taxonomy. Bacteria are classified and identified to distinguish among strains and to group them by criteria of interest to microbiologists and other scientists.. Faculty: Dr. Rakesh Sharda OBJECTIVES OF STAINING Improves visibiltiy by greater contrast between the organism and the background, differentiate various morphological types (by shape, size, arrangeme 3-. The Hanging-Drop Preparation. ; . Since you have been oriented to some basic tools and methods used in microbiology, we shall begin our study of microorganisms by learning how to prepare them for study of their morphology under the microscope.. . Essam. Bacterial Morphology. Bacteria: are unicellular free living organisms without . chlorophil. having . b. oth DNA and RNA . Bacterial . morphology. Can . be grouped into three types . 1- Spherical . refractile. body within the vegetative cells. Procedure: (fig.10) . 1- Prepare a fixed smear.. 2- Flood the smear with 5% malachite green and let the stain react for 1 minute .Using your . bunsen. burner, heat the top of the stained slide until steam rises but do not let it boil. This step is performed by passing the burner back and over the slide for about 5 minutes.. Fixatives used in histopathology. 2. Staining. Differential staining. Gram’s staining. Vital staining. Trypan. blue- dead cells take up the stain . i.e. stain positively and live cells . Exclude the stain or stain negatively.