PPT-Simple stain Bacterial Morphology

Bacteria are unicellular free living organisms without chlorophil having b oth DNA and RNA Bacterial morphology Can be grouped into three types 1 Spherical cocci 2 Rod . A stain is a substance that adheres to a cell, giving the cell color. .. The presence of color gives the cells significant contrast so are much more visible. . Different stains have different affinities for different organisms, or different parts of . Gram stain . Ziehl. . Neelsen. stain. . Requires the use of at least 3 chemical reagents.. Primary Stain. : . stain all the cells.. Decolorizing agent: . may or may not remove the primary stain from the entire the cell or only from certain cell structure. . BIOLOGY & BIOTECHNOLODY DEPARTMENT. In our laboratory, . bacterial morphology . (form and structure) may be examined in two ways:. By observing . living unstained organisms. (. wet mount. ).. 2. By . A stain is a substance that adheres to a cell, giving the cell color. .. The presence of color gives the cells significant contrast so are much more visible. . Different stains have different affinities for different organisms, or different parts of . .. The presence of color gives the cells significant contrast so are much more visible. . Different stains have different affinities for different organisms, or different parts of . organisms. They are used to differentiate different types of organisms or to view specific parts of organisms. Basic vs. acidic dyes. Differential Stain. Primary stain (stains all cells on slide). Decolorizing step (removes stain from certain types of cells). Counterstain (stains the decolorized cells). A.L. Noor Ameer . The reasons of staining. 1- To study their shapes . 2- . To differentiate the species of bacteria by using differential . stain.. 3- . To study the internal components of the bacterial . . Bacterial staining . Lab. 5 . A.L. Noor Ameer. Purpose of culturing is: . 1- Isolation of microorganisms from a . sample.. 2- . For counting the microorganism in the . sample.. 3- . Obtain pure . How to Focus the Microscope?. Turn the revolving nosepiece to 10 magnifying lens.. Move the stage up, the condenser to the top position and close iris diaphragm half way.. Put the slide on the slide holder on the stage.. tissues, cells, cell components, cell contents, or microorganisms.. *The stain may be . natural or synthetic. . . *The object stained is called the . substrate. . . *Staining facilitates the observation of a substrate by introducing . العملي. Lab -1. -2-. . Bacterial Taxonomy. Bacteria are classified and identified to distinguish among strains and to group them by criteria of interest to microbiologists and other scientists.. . Essam. Bacterial Morphology. Bacteria: are unicellular free living organisms without . chlorophil. having . b. oth DNA and RNA . Bacterial . morphology. Can . be grouped into three types . 1- Spherical . . hussin. Dept of pathology. 13 march 2018. H&E. . stain. . is. . routine. . stain.. It . is. . the. . preliminary . or. . the. . first. . stain. . applied. . to. . the. . tissue. sections. Bacterial Smear Preparation. It is a preparation that includes a . small amount of biological sample or culture . spread. . in a very thin film on the surface of the slide. . . Smear preparation is a step before staining..