PPT-Staining techniques 1. Fixation

Fixatives used in histopathology 2 Staining Differential staining Grams staining Vital staining Trypan blue dead cells take up the stain ie stain positively and live cells Exclude the stain or stain negatively. Neg a INTRODUCTION Negative staining is an easy, rapid, qualitative method for examining the structure of isolated organelles, individual macromolecules and viruses at the EM level. However, the meth HAEMATOXYLIN & EOSIN. . Supervised by : . Dr.Sherifa. . Prepared by: Reem Aldossari. Lama . Alabdi. H&E staining:. The best known and most widely used staining procedure, . hematoxylin. -eosin staining (H&E), uses . PRESENTER:DR.MUNENE. FACILITATOR:DR.MUTISO. -. Stability : degree of displacement at fracture site induced by load. -Stable fracture : fracture that does not visibly displace under physiological load. Spores . are resistant to heat and chemicals because of a tough outer covering made of the protein . keratin.. The keratin also resists staining, so extreme measures must be taken to stain the spore.. (BD Biosciences Protocol) Materials: Staining medium (SM) [1X HBSS; 2% (v/v) calf serum; 10mM NaN 3 10 mM HEPES, pH 7.2] Sterile filtered calf serum (CS) [0.45m TC sterile filtered] Gey’s solu Spores . are resistant to heat and chemicals because of a tough outer covering made of the protein . keratin.. The keratin also resists staining, so extreme measures must be taken to stain the spore.. between chambers and the stream reach. Hilary L. Madinger, Robert O. Hall. University of Wyoming. nitrogen fixation. N. 2. NH. 3. Organic C. photosynthesis. CO. 2. Low N . ecosystems. Nitrogen fixation requires energy input as carbon from photosynthesis. Lab #4 . Medgar Evers College. Bio 261 Microbiology. Prof. Santos. Aim #1 exercise 14. Capsular staining. Capsule- extracellular layer composed of polysaccharides found in some bacteria that plays a role in immunity and adhesion.. Faculty: Dr. Rakesh Sharda OBJECTIVES OF STAINING Improves visibiltiy by greater contrast between the organism and the background, differentiate various morphological types (by shape, size, arrangeme It is the branch of science which deals with the gross & microscopic study of normal tissue. Histopathology:. It is the branch of science which deals with the gross & microscopic study of tissue affected by disease.. This. procedure is called . so,when. certain bacteria or some of their . structures could not be stained or seen or being differential with other bacteria cells when using the ordinary . staining,such. Molecular nitrogen (N2) . is a very common chemical compound in which two nitrogen atoms are tightly bound together. . The . strong triple-bond between the atoms in molecular nitrogen makes this compound difficult to break apart, and thus nearly . . Immunohistochemistry. (. IHC. ):. The principle of IHC has been known since the 1930s, but it was not until 1942 that the first IHC study was reported. Coons . et al..  (1942) used FITC-labeled . Embedding. Sectioning. Staining. Fixation. It is important to . maintain. cells in as life-like a state as possible . to prevent post-mortem changes as a result of putrefaction (destruction of tissue by bacteria or fungi) and autolysis (destruction of tissue by its own enzymes). .