PPT-Staining techniques 1. Fixation

Fixatives used in histopathology 2 Staining Differential staining Grams staining Vital staining Trypan blue dead cells take up the stain ie stain positively and live cells Exclude the stain or stain negatively. HAEMATOXYLIN & EOSIN. . Supervised by : . Dr.Sherifa. . Prepared by: Reem Aldossari. Lama . Alabdi. H&E staining:. The best known and most widely used staining procedure, . hematoxylin. -eosin staining (H&E), uses . PRESENTER:DR.MUNENE. FACILITATOR:DR.MUTISO. -. Stability : degree of displacement at fracture site induced by load. -Stable fracture : fracture that does not visibly displace under physiological load. Techniques. By. DR ANYANWU GE. Introduction. Histological technique deals with the preparation of tissue for microscopic examination. . The aim of good histological technique to preserve microscopic anatomy of tissue. . between chambers and the stream reach. Hilary L. Madinger, Robert O. Hall. University of Wyoming. nitrogen fixation. N. 2. NH. 3. Organic C. photosynthesis. CO. 2. Low N . ecosystems. Nitrogen fixation requires energy input as carbon from photosynthesis. Staining. Coloring organism with a dye. Microorganisms must be . FIXED. to microscopic slide first. Kills microbe. Attaches organism to the slide. Preserves various parts with minimal distortion. Steps to fix:. Zahoor Ahmed. B.S MLT 06. Introduction. Histological technique deals with the preparation of tissue for microscopic examination. . The aim of good histological technique to preserve microscopic anatomy of tissue. . & . Antibiotic susceptibility testing. : An introduction. Dr. Mohit Bhatia. Assistant Professor. Department of Microbiology. AIIMS, Rishikesh. Staining techniques . Smear preparation. Smear = 2 × 3 cm in size. . .. Dr. Archana . Kumari. Asstt. . Professor cum Junior Scientist. Veterinary Surgery and Radiology. BVC, BASU, Patna. METHOD OF EXTERNAL FRACTURE FIXATION . .. Robert jones bandage . . . Ehmer. It is the branch of science which deals with the gross & microscopic study of normal tissue. Histopathology:. It is the branch of science which deals with the gross & microscopic study of tissue affected by disease.. This. procedure is called . so,when. certain bacteria or some of their . structures could not be stained or seen or being differential with other bacteria cells when using the ordinary . staining,such. These techniques included:. *Anesthetization. *Fixation. *Embedding. *cutting. *mounting onto slides. *Staining. *Washing. *mounting . *preservation. Animal experiments are necessary for a better understanding of diseases and for developing new therapeutic strategies. Animal experiment include Mouse, Rat, Rabbit, Hamster,..... Molecular nitrogen (N2) . is a very common chemical compound in which two nitrogen atoms are tightly bound together. . The . strong triple-bond between the atoms in molecular nitrogen makes this compound difficult to break apart, and thus nearly . . Immunohistochemistry. (. IHC. ):. The principle of IHC has been known since the 1930s, but it was not until 1942 that the first IHC study was reported. Coons . et al..  (1942) used FITC-labeled . Embedding. Sectioning. Staining. Fixation. It is important to . maintain. cells in as life-like a state as possible . to prevent post-mortem changes as a result of putrefaction (destruction of tissue by bacteria or fungi) and autolysis (destruction of tissue by its own enzymes). .