PPT-Staining

HAEMATOXYLIN amp EOSIN Supervised by DrSherifa Prepared by Reem Aldossari Lama Alabdi HampE staining The best known and most widely used staining procedure hematoxylin eosin staining HampE uses . Neg a INTRODUCTION Negative staining is an easy, rapid, qualitative method for examining the structure of isolated organelles, individual macromolecules and viruses at the EM level. However, the meth (BD Biosciences Protocol) Materials: Staining medium (SM) [1X HBSS; 2% (v/v) calf serum; 10mM NaN 3 10 mM HEPES, pH 7.2] Sterile filtered calf serum (CS) [0.45m TC sterile filtered] Gey’s solu GUIDELINES Silver staining is used for sensitive detection of proteins separated by 1D and 2D SDS PAGE with detection limits from 0.5-5 ng. Many silver staining protocols and commercial staining Lab #4 . Medgar Evers College. Bio 261 Microbiology. Prof. Santos. Aim #1 exercise 14. Capsular staining. Capsule- extracellular layer composed of polysaccharides found in some bacteria that plays a role in immunity and adhesion.. Staining. Coloring organism with a dye. Microorganisms must be . FIXED. to microscopic slide first. Kills microbe. Attaches organism to the slide. Preserves various parts with minimal distortion. Steps to fix:. A.L. Noor Ameer . The reasons of staining. 1- To study their shapes . 2- . To differentiate the species of bacteria by using differential . stain.. 3- . To study the internal components of the bacterial . Ef31cientBest-in-Class Productivity149True walk-away solution designed for LEAN processes149Continuous loading and unloading evens out your workload149Unique rack design facilitating efficient workflo NEGATIVECONTROLREAGENTMONOCLONALRABBITANTI-PD-L1CLONE28-8PEROXIDASE-BLOCKINGREAGENTP0416301/SK00521-5/201510 p2/131 x 345mLLINKER Anti-RabbitMouse secondary antibody against rabbit immunoglobulins in for paraffin embedded tissueinfoarigobiocomwwwarigobiocom1/4Materials and Reagentsused in this protocolDescriptionFormalin or other aldehyde fixativesEx Formaldehyde glutaraldehyde forms protein cross Faculty: Dr. Rakesh Sharda OBJECTIVES OF STAINING Improves visibiltiy by greater contrast between the organism and the background, differentiate various morphological types (by shape, size, arrangeme 4327 Abstract.OBJECTIVE: Erythropoietin (EPO), as a type of the tissue-protective cytokines, is a 30.4 kDa hematopoietic glycoprotein. The purpose of this study was to explore the neuroprotective effe This. procedure is called . so,when. certain bacteria or some of their . structures could not be stained or seen or being differential with other bacteria cells when using the ordinary . staining,such. . Immunohistochemistry. (. IHC. ):. The principle of IHC has been known since the 1930s, but it was not until 1942 that the first IHC study was reported. Coons . et al..  (1942) used FITC-labeled . Fixatives used in histopathology. 2. Staining. Differential staining. Gram’s staining. Vital staining. Trypan. blue- dead cells take up the stain . i.e. stain positively and live cells . Exclude the stain or stain negatively.