PPT-Staining Microorganisms An overview of staining

Staining Coloring organism with a dye Microorganisms must be FIXED to microscopic slide first Kills microbe Attaches organism to the slide Preserves various parts with minimal distortion Steps to fix. A. Contaminants – microbes present at a given place and time that is undesirable. B. Decontamination – the removal or destruction of contaminants. C. Terminology. 1. Sterilization. A) Process that removes all viable microorganisms including viruses & endospores. Prof. . Mohammad . Shubair. Microorganisms get into food from both natural sources and from external sources to which a food comes into contact from the time of production until the time of consumption.. Are they harmful?. Are they beneficial?. Microorganisms. Micro. So small you can’t see it!. Organisms . It’s alive!. Which are harmful?. Which are beneficial?. Beneficial Microorganisms . Algae. grows on water and produces oxygen. 2012. Mrs. Erickson. Science Benchmark. Microorganisms. . are those . living. things that are . visible. as individual organisms only with the . aid of magnification. . Microorganisms are components of . Chapter 1. Lesson 5. Microorganisms. Microorganisms are organisms that are microscopic, or too small to be seen with just the eyes. . They can be unicellular or multicellular.. Microorganisms are EVERYWHERE!!! They are in food that you eat, on your books, and inside your body. . . NATURE OF BIOTECHNOLOGY AND. INDUSTRIAL MICROBIOLOGY. One of the broadest . definition of Biotechnology. is the one given at the United Nations Conference on Biological Diversity in 1992 as . “any technological application that uses biological systems, living organisms, or derivatives thereof, to make or modify products or processes for specific use.”. Lab #4 . Medgar Evers College. Bio 261 Microbiology. Prof. Santos. Aim #1 exercise 14. Capsular staining. Capsule- extracellular layer composed of polysaccharides found in some bacteria that plays a role in immunity and adhesion.. lead to . surgical site infections . (SSIs) may . either be present on the patient’s skin or mucous membranes or transmitted to the patient by health care personnel, the environment, or other items in the perioperative setting. . Booster antibody and ELISA experts is a remarkable and destination which provide accurate and simple Intracellular Staining Protocol. It happens every year sometimes two or three times It happens to almost everyone in every city state town and country around the world When was the last time it happened to you What Oh Microteach. Long Chen. What is your intuition to define a microorganism?. Tiny . Simple . Germs cause disease. The story begins with … …. “The Father of Microbiology”.  . Antonie. van Leeuwenhoek. the. . Culture media as a fellow: An artificial environment simulating natural conditions that necessary for bacteria to grow in laboratory. . . Components of the Typical Culture Medium. :. . 1- Energy source . a. Construct an argument using scientific evidence to support a claim that microorganisms are beneficial.. b. Construct an argument using scientific evidence to support a claim that microorganisms are harmful.. Fixatives used in histopathology. 2. Staining. Differential staining. Gram’s staining. Vital staining. Trypan. blue- dead cells take up the stain . i.e. stain positively and live cells . Exclude the stain or stain negatively.