PPT-Staining Microorganisms An overview of staining

Staining Coloring organism with a dye Microorganisms must be FIXED to microscopic slide first Kills microbe Attaches organism to the slide Preserves various parts with minimal distortion Steps to fix. Neg a INTRODUCTION Negative staining is an easy, rapid, qualitative method for examining the structure of isolated organelles, individual macromolecules and viruses at the EM level. However, the meth HAEMATOXYLIN & EOSIN. . Supervised by : . Dr.Sherifa. . Prepared by: Reem Aldossari. Lama . Alabdi. H&E staining:. The best known and most widely used staining procedure, . hematoxylin. -eosin staining (H&E), uses . (BD Biosciences Protocol) Materials: Staining medium (SM) [1X HBSS; 2% (v/v) calf serum; 10mM NaN 3 10 mM HEPES, pH 7.2] Sterile filtered calf serum (CS) [0.45m TC sterile filtered] Gey’s solu GUIDELINES Silver staining is used for sensitive detection of proteins separated by 1D and 2D SDS PAGE with detection limits from 0.5-5 ng. Many silver staining protocols and commercial staining Lab #4 . Medgar Evers College. Bio 261 Microbiology. Prof. Santos. Aim #1 exercise 14. Capsular staining. Capsule- extracellular layer composed of polysaccharides found in some bacteria that plays a role in immunity and adhesion.. A.L. Noor Ameer . The reasons of staining. 1- To study their shapes . 2- . To differentiate the species of bacteria by using differential . stain.. 3- . To study the internal components of the bacterial . Ef31cientBest-in-Class Productivity149True walk-away solution designed for LEAN processes149Continuous loading and unloading evens out your workload149Unique rack design facilitating efficient workflo NEGATIVECONTROLREAGENTMONOCLONALRABBITANTI-PD-L1CLONE28-8PEROXIDASE-BLOCKINGREAGENTP0416301/SK00521-5/201510 p2/131 x 345mLLINKER Anti-RabbitMouse secondary antibody against rabbit immunoglobulins in for paraffin embedded tissueinfoarigobiocomwwwarigobiocom1/4Materials and Reagentsused in this protocolDescriptionFormalin or other aldehyde fixativesEx Formaldehyde glutaraldehyde forms protein cross Faculty: Dr. Rakesh Sharda OBJECTIVES OF STAINING Improves visibiltiy by greater contrast between the organism and the background, differentiate various morphological types (by shape, size, arrangeme 4327 Abstract.OBJECTIVE: Erythropoietin (EPO), as a type of the tissue-protective cytokines, is a 30.4 kDa hematopoietic glycoprotein. The purpose of this study was to explore the neuroprotective effe This. procedure is called . so,when. certain bacteria or some of their . structures could not be stained or seen or being differential with other bacteria cells when using the ordinary . staining,such. . Immunohistochemistry. (. IHC. ):. The principle of IHC has been known since the 1930s, but it was not until 1942 that the first IHC study was reported. Coons . et al..  (1942) used FITC-labeled . Fixatives used in histopathology. 2. Staining. Differential staining. Gram’s staining. Vital staining. Trypan. blue- dead cells take up the stain . i.e. stain positively and live cells . Exclude the stain or stain negatively.