PPT-Collection of blood and blood serum,

ruminal fluid for computing metabolic profile test Dr Egendra K shrestha M V Sc Blood constitutes cell and plasma The cells are erythrocytes leucocytes and platelets Plasma contains fibrinogen globulin and albumin as well as . Compiled by: . Thamer. . Hamdan. M.Sc. Clinical Microbiology and Immunology. Interference. occurs when a substance or process falsely alters an assay . result.. Interferences are classified as endogenous or exogenous.. Protein . Nitrogen (NPN) . Concentration. 224 PHL. Lab#5 . Non-protein nitrogen (NPN). NPN includes . the nitrogen from all nitrogenous substances . other than proteins. ..  . The NPN could be measured as a . Total Protein . Concentration. 224 PHL. Lab#. 2. . 1) Simple Proteins:. . They yield only . α. -amino acids . on hydrolysis.. e.g. . albumin, globulin. .. 2) Conjugated Proteins:. . They are proteins combined with a non-protein substance called prosthetic group. . Protein Electrophoresis . (SPEP). Lab 3. What does it mean ?. Serum protein electrophoresis is an easy method of separating serum proteins based on their net charge, size, and shape. . A small amount of serum is placed on a specific medium (such as agarose) and an electrical charge is applied. . and its Supplements. Introduction. . Animal Cell Culture can be described as . in vitro maintenance and propagation of animal cell using a suitable nutrient media. . Culturing is the process of growing animal cell artificially.. INDEX. Intoduction to culture media.. Components of serum.. Advantages and disadvantages of serum media.. Advantages and disadvantages of serum free media.. CULTURE MEDIA = defined as growth media suitable for the in- vitro cell culturing of human or animal cells and the composition of components are known.. Issues with Serum based Media . Serum.  is vitally important as a naturally available source of growth and adhesion factors, hormones, lipids and minerals for the culture of cells in basal media..   . Removal. Preventing Infection. Needle-Free Valves. Insertion. Central Line Bundle. Care & Maintenance. TPN. Flushes. Are central lines driving you crazy?. . What you need to know when a central line is necessary…. Anne . Barasa, . MBChB, . MMed (Path. ). Lecturer, Immunology . Unit. Department of Human Pathology. University of . Nairobi. KPA 2019. University of Nairobi . . Antibody testing: BVD-1 Antibody titer : Value BVD-2 Antibody titer : Value Growth promotion and cytotoxicity: Each lot of serum is tested for growth promotion and cytoto Lecture : 1. ////Dr. . . Shaimaa. . Munther. . Clinical biochemistry. . Clinical chemistry refers to the biochemical analysis of body fluids. It uses chemical reactions to determine the levels of various chemical compounds in bodily fluids. . Immunofixation. Dr.Ajay. . Phadke. Centre Head. SRL Diagnostics-. Dr.Avinash. . Phadke’s. Lab. What is electrophoresis?. Electrophoresis is a method of separating proteins based on their physical properties. Casadevall A. Antibody-Based Therapies for Emerging Infectious Diseases. Emerg Infect Dis. 1996;2(3):200-208. https://doi.org/10.3201/eid0203.960306. Hieu Bui and John . Reif. Duke University. The success of DNA nanotechnology comes from three key ingredients:. Our quantitative understanding of DNA thermodynamics. , which makes it possible to predict reliably how single-stranded DNA molecules fold and interact with one another.