PPT-DNA Devices in Serum and the Cell

Hieu Bui and John Reif Duke University The success of DNA nanotechnology comes from three key ingredients Our quantitative understanding of DNA thermodynamics which makes it possible to predict reliably how singlestranded DNA molecules fold and interact with one another. Cleansing serum . . เซรั่ม. ทำความสะอาด. ผิวหน้าสูตร . Make. . Up. . Remover. . . เนื้อบางเบา เปี่ยมประสิทธิภาพ. Protein . Nitrogen (NPN) . Concentration. 224 PHL. Lab#5 . Non-protein nitrogen (NPN). NPN includes . the nitrogen from all nitrogenous substances . other than proteins. ..  . The NPN could be measured as a . Total Protein . Concentration. 224 PHL. Lab#. 2. . 1) Simple Proteins:. . They yield only . α. -amino acids . on hydrolysis.. e.g. . albumin, globulin. .. 2) Conjugated Proteins:. . They are proteins combined with a non-protein substance called prosthetic group. . Protein Electrophoresis . (SPEP). Lab 3. What does it mean ?. Serum protein electrophoresis is an easy method of separating serum proteins based on their net charge, size, and shape. . A small amount of serum is placed on a specific medium (such as agarose) and an electrical charge is applied. . and. . Separation of Main Proteins in Plasma and Serum . Practical . of the . First and Second Experiments. Objectives. Plasma Separation, and calculation of percentage . Serum Separation, . and calculation of . INDEX. Intoduction to culture media.. Components of serum.. Advantages and disadvantages of serum media.. Advantages and disadvantages of serum free media.. CULTURE MEDIA = defined as growth media suitable for the in- vitro cell culturing of human or animal cells and the composition of components are known.. Issues with Serum based Media . Serum.  is vitally important as a naturally available source of growth and adhesion factors, hormones, lipids and minerals for the culture of cells in basal media..   . DNA Extraction Process involves four parts. :. Put the cells into a solution.. Use enzymes to hydrolyze the cell wall.. Use a detergent to break apart the cell membrane.. Use 95% ethanol to take out the DNA from the lysate.. Do Now. All the cells in our body have the same DNA, yet you have:. Muscle Cells. Neurons. Lung Epithelial Cells. How is this possible?. Cells specialize like we do!. Baby. Law Student. Art School Student. eGFR. . Where . A. re . W. e Now?. Dr Mike Bosomworth. Lead Clinician - Blood Sciences. Leeds Teaching Hospitals. 16. th. April 2013. 1. 2. Why measure serum . creatinine. ?. RENAL CLEARANCE. Substance cleared by renal excretion clearance (Cy ):-.  . Author: Michael Fenech. Affiliation: Genome Health Foundation, North Brighton, SA, 5048, Australia. Email: . mf.ghf@outlook.com. . Introduction. Life as we know it depends entirely on the capacity of cells to utilize energy and molecules in the environment for cellular function and reproduction. . Chapters 12, 13, 16, 17. Limits to Cell Growth. The larger a cell becomes, the more demands a cell places on its DNA. If extra copies of DNA are not made, an “information crisis” would occur. The cell also has more trouble moving nutrients and wastes across the cell membrane. Get a sheet of paper and . number . 1-29. Answer are on the last slide. 1. were . credited for finding the structure of DNA. Chargaff. Franklin. Watson . & . Crick. 2. What phase of Mitosis is this?. ruminal. fluid for computing metabolic profile test. Dr . Egendra. K . shrestha. M V Sc. Blood . constitutes cell and plasma. The cells are erythrocytes, leucocytes and platelets. Plasma contains fibrinogen, globulin and albumin as well as .