PPT-Serum Protein Electrophoresis with

Immunofixation DrAjay Phadke Centre Head SRL Diagnostics DrAvinash Phadkes Lab What is electrophoresis Electrophoresis is a method of separating proteins based on their physical properties. Chelsea Aitken. Peter Aspinall. Zonal Electrophoresis. Most common form of electrophoresis in biological studies. Uses a support system, most commonly gel to separate proteins by their properties. We will cover methods to separate by:. Protein Electrophoresis . (SPEP). Lab 3. What does it mean ?. Serum protein electrophoresis is an easy method of separating serum proteins based on their net charge, size, and shape. . A small amount of serum is placed on a specific medium (such as agarose) and an electrical charge is applied. . INTRODUCTION. Electrophoresis is a migration of a charged particle in a medium under the influence of an applied electric field.. The usual purposes of carrying out . electrophoretic. experiments are :. Tasnuva. . Jhileek. Dr. Francine . Norflus. Biotechnology. What is Gel Electrophoresis?. A procedure that separates molecules . based on size and charge.. . Uses an electric field. Molecules move through . . Afreen. Hasan . Electrophoresis is the movement of charged particles through a porous supporting media when subjected to an electric Field . • Cations move towards cathode. • Anions move towards anode . المدرس المساعد:نيران علي . ثامر. ماجستير كيمياء حياتية . سريرية. protein; isolation ;purification. Chrom. a. tography. Electrophoresis. I. . Protein Isolation. Proteins . Carbohydrates . Nucleic acid. Polysaccharides. Peptides. Amino acids. Oligosaccharides. Nucleosides. Organic acids. Small anions and cat ions of body fluids .. Principle of E. lectrophoresis . 1. Migration of charged particles on supporting media. Migration of charged particles in solution. No supporting media. 2. GEL ELECTROPHORESIS. Sep. Of proteins Based on molecular wt only. Based on mol. Wt. & . Joannes F.M. Jacobs and Katherine A. Turner contributed equally to this work.*Corresponding authors: Joannes F.M. Jacobs, PhD, MD, Laboratory Medical Immunology, Department of Laboratory Medicine, Cl Dr. D Y Patil Pimpri Pune 411018 Chemistry Department Mrs. Tarannum S Attar CONTENT Introduction Principle Factors affecting Conventional electrophoresis General operation Technical and prac Electrophoresis is a migration of a charged particle in a medium under the influence of an applied electric field.. The usual purposes of carrying out . electrophoretic. experiments are :. 1. To determine the number, amount and mobility of components in a given sample or to separate them.. Danielle R. . Snowflack. , Ph.D.. www.edvotek.com. EDVOTEK. The . Biotechnology Education. Company. Celebrating OVER 30 years . of science education!. Proteins are everywhere!. Proteins are diverse molecules that perform important functions within a cell. By Angel Luis Vázquez Maldonado. November 8. th. , 2018. Gel Electrophoresis and its Purpose. 2. Electrophoresis is derived from Greek. Electro . – refers to the electrical current that adds energy to the electrons of the molecule’s atoms. Peter Aspinall. Zonal Electrophoresis. Most common form of electrophoresis in biological studies. Uses a support system, most commonly gel to separate proteins by their properties. We will cover methods to separate by:.