PPT-Biotechnology Basics: Protein Electrophoresis

Danielle R Snowflack PhD wwwedvotekcom EDVOTEK The Biotechnology Education Company Celebrating OVER 30 years of science education Proteins are everywhere Proteins are diverse molecules that perform important functions within a cell. By Andrew Gioe and Ben Berger. Electrophoretic Experiments. Free Electrophoresis or Moving Boundary Electrophoresis. Done in solution with no support Medium. No longer widely used due to problems resulting from the formation of convection currents in the solution from heating.. Ayat. . Zawawi. Principle. Factors affecting the distance of movement. Application. Polyacrylamide Gel Electrophoresis (PAGE). Hemoglobin Electrophoresis. Objectives. Electrophoresis is a process distinguishing and isolating different compounds from each other. . Chelsea Aitken. Peter Aspinall. Zonal Electrophoresis. Most common form of electrophoresis in biological studies. Uses a support system, most commonly gel to separate proteins by their properties. We will cover methods to separate by:. INTRODUCTION. Electrophoresis is a migration of a charged particle in a medium under the influence of an applied electric field.. The usual purposes of carrying out . electrophoretic. experiments are :. . Afreen. Hasan . Electrophoresis is the movement of charged particles through a porous supporting media when subjected to an electric Field . • Cations move towards cathode. • Anions move towards anode . Martin Cole (. isoelectric. focusing), . Mcolisi. . Dlamini. , . Faraz. Khan. April 18. 2012. Physics 200: Molecular Biophysics. http://vadlo.com/cartoons.php?id=445. What does it do?. Separation of. المدرس المساعد:نيران علي . ثامر. ماجستير كيمياء حياتية . سريرية. protein; isolation ;purification. Chrom. a. tography. Electrophoresis. I. . Protein Isolation. Danielle R. . Snowflack. , Ph.D.. www.edvotek.com. EDVOTEK. The . Biotechnology Education. Company. Celebrating OVER 30 years . of science education!. Today’s Experiment. Restriction Enzymes allow researchers precisely cut DNA. Proteins . Carbohydrates . Nucleic acid. Polysaccharides. Peptides. Amino acids. Oligosaccharides. Nucleosides. Organic acids. Small anions and cat ions of body fluids .. Principle of E. lectrophoresis . 1. Migration of charged particles on supporting media. Migration of charged particles in solution. No supporting media. 2. GEL ELECTROPHORESIS. Sep. Of proteins Based on molecular wt only. Based on mol. Wt. & . 1 2 Gel Electrophoresis, Principle, Types and Applications Description of Module Subject Name Paper Name Module Name/Title Gel Electrophoresis, Principle, Types and Applications Dr. Vijaya Khader Dr. To prepare 4% NuSieve agarose gel (14 x 10 x 0.5 cm) take the following in a 500 ml Agarose: 1.6 g NuSieve agarose: 1.6 g 1 X AGB buffer 80 ml Fill the electrophoresis tank with 1 x TBE buffer. Plac Principle. Factors affecting the distance of movement. Application. Polyacrylamide Gel Electrophoresis (PAGE). Hemoglobin Electrophoresis. Objectives. Electrophoresis is a process distinguishing and isolating different compounds from each other. . Peter Aspinall. Zonal Electrophoresis. Most common form of electrophoresis in biological studies. Uses a support system, most commonly gel to separate proteins by their properties. We will cover methods to separate by:.