PPT-An introduction to CRISPR gene engineering

Introduction to CRISPR http wwwaddgeneorg CRISPRguide Wiedenheft et al Nature 2012 3 distinct types of bacterial CRISPR systems identified so far Type I II III Type II is the basis for current genome engineering applications. DeMayo. FJ, Spencer TE. Jennifer Thornton. April 1, 2015. Happy April Fools’ Day!. *I won’t actually cover this paper, but it’s real and you should check it out at. http. ://www.ncbi.nlm.nih.gov/pubmed/25100711. - through the lens of the IRB -. P. Pearl O’Rourke, MD. Partners HealthCare. Boston, MA. Context:. Institutional Review Board (IRB). Charged with the oversight of . human subjects . research. Common Rule, definition of human subject : . Type . III-B to . Target RNA Encoded . Viruses infecting humans. . Presented By: Diana Marquez. Rhinovirus. Common cold. Impacts adults 2-3 times a year and children 6-10.. Introduction. + stranded RNA virus.. The Wonderful World of CRISPR. As told by Professor Peter Shepherd. To do precise genetic engineering we need to be able to find and specifically modify regions of DNA. But the human genome has 3,000,000,000 base pairs so how are we going to find a 20 base pair region in this huge sea of DNA ? . Ruairidh Barlow. What is Parkinson's Disease?. Age related neurodegenerative disease. Symptoms: . tremors, stiffness, and slow movement. Substantia . Nigra. Pars Compacta. Death of dopaminergic neurons is a key feature in the pathology of the disease. Viruses. . Presented By: Diana Marquez. Introduction. The Rhinovirus, while often synonymous with the “common cold” has also been found to be a contributing factor for the occurrence of acute respiratory conditions such as asthma and inhibit effective infection resolution in at risk individuals. How can we advance CRISPR from a revolutionary research tool to a level of precision and efficiency that will enable us to target ANY genetic disease?. DUAL OMNI™ . TECHNOLOGY PLATFORMS. Novel CRISPR Nuclease Discovery Platform. A Neiteler. 1,2,3,5. , S Borooah. 6. , BT Selvaraj. 2,3,5. , K Burr. 2,3,5. , B Dhillon. 2,4. , JA Ross. 1. , S Chandran. 2,3,5. 1. Tissue Injury and Repair Group, . 2. Centre for Clinical Brain Sciences, . González. Master in . Advanced. . Genetics. Universitat. . Autònoma. de Barcelona. GENOMICS. Introduction. Francisco J. M. Mojica . CRISPR. 2000. Source. : . Doudna. . y . Charpentier. , 2014. Introduction. Modern methods in Molecular Biology. . Epigenetisc. Crispr. /CAs9. RNAi. Epigenetic  refers to heritable changes in gene expression (active versus inactive genes), that does not involve changes to the underlying DNA sequence; a change in phenotype without a change in genotype. . Retina Summary of Grant Activities: January to December 2015 Project Title: Correcting inherited retinal disease through gene editing . Research Team: Dr Sandy Hung, Dr Raymond Wong, Dr Kathryn D References. Eberl. , G. . et al. . Innate lymphoid cells: A new paradigm in immunology. . Science. . 348. , 879-887 (2015).. Joung. , J. . et al. . Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. . Natronobacterium. . gregoryi. . Argonaute. Feng Gao, Xiao Z Shen, Feng Jiang, . Yongqiang. Wu & . Chunyu. Han. Gene . E. diting Tools Overview. https://en.wikipedia.org/wiki/Genome_editing. In order to do a targeted genome editing, we need to make a double stranded breaks at specific sites within genome. Pereyra-Bonnet Federico BSc, PhD. . Co-Founder of CASPR. . National Researcher of CONICET, Buenos Aires University, Argentina.. 2019, SINGAPURE. CRISPR. PRECISION. MOLECULAR. DIAGNOSTICS. Target . sequence.