PPT-An introduction to CRISPR gene engineering

Introduction to CRISPR http wwwaddgeneorg CRISPRguide Wiedenheft et al Nature 2012 3 distinct types of bacterial CRISPR systems identified so far Type I II III Type II is the basis for current genome engineering applications. Presented By :. Amna. Muhammad. 09-Arid-1536. Ph. D Scholar . Biochemistry. 1. st. Semester. 12/16/2014. 1. Contents. History. Functional analysis of a gene. RNA . interfernce. . siRNA. shRNA. miRNA. Breakthrough in genome editing.. The most important scientific result . of the year 2015.. “Science“,18 December 2015. I will report first about. Duchenne muscular dystrophy,. because there were serious problems . Nick Turner. Bio 446 Fall 16. Review. siRNA. dsRNA. microRNA. Immunity. Gene Regulation. Enzymatic breakdown of RNA. RNA Interference. RNAi. The use of RNA to inhibit gene expression.. Guiding RISC (RNA Induced Silencing Complex) cleave and degrade specific segments of RNA. The Wonderful World of CRISPR. As told by Professor Peter Shepherd. To do precise genetic engineering we need to be able to find and specifically modify regions of DNA. But the human genome has 3,000,000,000 base pairs so how are we going to find a 20 base pair region in this huge sea of DNA ? . Darryl Benjamin. Real Food Seminars. Montpelier, Vermont. darryl@realfoodseminars.com. 802 585 5855. Updated 08.09.17. What is CRISPR?. CRISPR/Cas9. , is essentially a pair of molecular scissors for . CRISPR Babies: Background and Ethics By: Austin Guse and Kaitlyn Curtis What is a CRISPR baby? An organism, specifically a human child, that has had its genetics altered in one way or another and has successfully been born or is still in guestation. FucU CRIPR/Cas9 KO Plasmid (m): sc-427279 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe + 00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGR How can we advance CRISPR from a revolutionary research tool to a level of precision and efficiency that will enable us to target ANY genetic disease?. DUAL OMNI™ . TECHNOLOGY PLATFORMS. Novel CRISPR Nuclease Discovery Platform. A Neiteler. 1,2,3,5. , S Borooah. 6. , BT Selvaraj. 2,3,5. , K Burr. 2,3,5. , B Dhillon. 2,4. , JA Ross. 1. , S Chandran. 2,3,5. 1. Tissue Injury and Repair Group, . 2. Centre for Clinical Brain Sciences, . for creation of. p53 knock-outs in . human . glioma . cells. :. a. research proposal by . gus. . thomas. Overview and Introduction:. Glioblastoma Multiforme (GBM):. Aggressive brain cancer. Very poor prognosis. Modern methods in Molecular Biology. . Epigenetisc. Crispr. /CAs9. RNAi. Epigenetic  refers to heritable changes in gene expression (active versus inactive genes), that does not involve changes to the underlying DNA sequence; a change in phenotype without a change in genotype. . Chapter 20 - Genome Defense. Figure 20.01. . Antisense RNA. Can Base Pair With mRNA. mRNA is normally made using the noncoding strand of DNA as a . template. . Such mRNA is also known as . sense RNA. . Carl Pray. 1. Can advances in biology transform Asian agriculture? Challenges are large. Food production . Productivity growth . Crises – . Biological -Africa Swine Fever, corn stalk borer, fall armyworm, wheat rust. ?. G. enetic . engineering. , the artificial manipulation, modification, and recombination of DNA or other nucleic acid molecules in order to modify an organism or population of organisms. . This . may mean changing one base .