Daniel Nicholson Graduate Student Department of Paper amp Bioprocess Engineering ESF Biodiesel Production Program The current US biodiesel production capacity is nearing 1 billion gal ID: 590724
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Slide1
Use of Biodiesel Waste Glycerol in the Bacterial Production of Polyhydroxyalkanoate (PHA) Biodegradable Plastic
Daniel Nicholson Graduate Student, Department of Paper & Bioprocess EngineeringSlide2
ESF Biodiesel Production Program
The current US biodiesel
production capacity
is nearing
1 billion gal
per
year
.
The co-product stream of waste glycerol could surpass 100 million gal per year in the near future, and many possible uses for this essentially free raw material are under investigation.
Biodiesel is produced on the SUNY ESF campus
from waste fryer oil from SU food services, for use in college vehicles and equipment .
Triglyceride
Transesterification
90% Biodiesel
Fatty acid methyl ester
10%
Glycerol
+Slide3
Polyhydroxyalkanoate (PHA) Chemistry
R = CH
3
:
Hydroxybutyrate
(HB)
R = CH
2
CH3: Hydroxyvalerate (HV)R = 3-6 Carbon side chain: Medium chain length (mcl) PHAs with more elastomeric properties. Increased side chain (R) length imparts flexibility to the polymer.Microbiologically produced poly-3-hydroxyalkanoate (PHA) polymers are a well known biodegradable material with physical characteristics comparable to some commercially available plastics like polypropylene and polyethylene. Slide4
PHA Biodegradability
Biodegradability studies have shown that many bacteria and fungi excrete PHA degrading enzymes in soil, sludge, fresh and
sea water. These enzymes hydrolyze solid PHA into water-soluble oligomers and monomers, and use the resulting products as nutrients within their
cells
.
Jendrossek, D. 2001. Adv. Biochem Eng Biotechnol. 71. 294-325
Weeks in sewage sludge
0
2
4
6
8Slide5
PHA Production Life CycleSlide6
Bacterial PHA Production
Consumes a number
of foods, and stores
extra in granules.
Benchtop bioreactors can be used to carefully
control growth
, and to observe
product formation
and
food consumption.
Burkholderia cepacia is a bacteria common in soil and water. This organism is being used in research at SUNY ESF, and has been shown to produce PHAsfrom different food sources.
PHA is the material stored to
conserve energy and Carbon for
future use.Slide7
Biotechnology @ ESF
Current projects
Metabolic and protein engineering for the production of biodegradable plastics
Microbial biodiesel production
Engineering of
cellulase
enzymes
Looking for graduate student applicants with strong backgrounds in:
Biochemistry
Molecular Biology
Microbiology
Synthetic organic chemistry
For more information you can email Dr. Nomura
ctnomura@esf.edu
Lab webpage:
http://www.esf.edu/chemistry/nomura/lab/