PPT-Figure Figure. . Pulsed-field gel electrophoresis patterns of the three clinical isolates

Bastida T PérezVázquez M Campos J CortésLletget M Román F Tubau F et al Levofloxacin Treatment Failure in Haemophilus influenzae Pneumonia Emerg Infect Dis 200391114751479 httpsdoiorg103201eid0911030176. What is it, and . how does it work?. Technique used to separate samples of DNA, RNA, and protein according to charge and/or size. Smaller molecules move farther and faster through the . agarose. gel. Electrophoresis. • . Separation technique based on the movement of . analyte. through a conductive medium in response to an applied electrical field.. • . The medium is usually a buffered aqueous. Chelsea Aitken. Peter Aspinall. Zonal Electrophoresis. Most common form of electrophoresis in biological studies. Uses a support system, most commonly gel to separate proteins by their properties. We will cover methods to separate by:. Tutorial. lise schoonen. 14-12-’15. 1. What is gel electrophoresis?. Method for separation and analysis of macromolecules. DNA, RNA, proteins. Separation based on size and/or charge. Electric field. INTRODUCTION. Electrophoresis is a migration of a charged particle in a medium under the influence of an applied electric field.. The usual purposes of carrying out . electrophoretic. experiments are :. Tasnuva. . Jhileek. Dr. Francine . Norflus. Biotechnology. What is Gel Electrophoresis?. A procedure that separates molecules . based on size and charge.. . Uses an electric field. Molecules move through . . Afreen. Hasan . Electrophoresis is the movement of charged particles through a porous supporting media when subjected to an electric Field . • Cations move towards cathode. • Anions move towards anode . lise schoonen. 14-12-’15. 1. What is gel electrophoresis?. Method for separation and analysis of macromolecules. DNA, RNA, proteins. Separation based on size and/or charge. Electric field. Marker can be used to determine size of sample. 1. Migration of charged particles on supporting media. Migration of charged particles in solution. No supporting media. 2. GEL ELECTROPHORESIS. Sep. Of proteins Based on molecular wt only. Based on mol. Wt. & . Electrophoresis is a migration of a charged particle in a medium under the influence of an applied electric field.. The usual purposes of carrying out . electrophoretic. experiments are :. 1. To determine the number, amount and mobility of components in a given sample or to separate them.. Principle. Factors affecting the distance of movement. Application. Polyacrylamide Gel Electrophoresis (PAGE). Hemoglobin Electrophoresis. Objectives. Electrophoresis is a process distinguishing and isolating different compounds from each other. . By Angel Luis Vázquez Maldonado. November 8. th. , 2018. Gel Electrophoresis and its Purpose. 2. Electrophoresis is derived from Greek. Electro . – refers to the electrical current that adds energy to the electrons of the molecule’s atoms. Tassios PT, Chadjichristodoulou C, Lambiri M, Kansouzidou-Kanakoudi A, Sarandopoulou Z, Kourea-Kremastinou J, et al. Molecular Typing of Multidrug-Resistant Salmonella Blockley Outbreak Isolates from Greece. Emerg Infect Dis. 2000;6(1):60-64. https://doi.org/10.3201/eid0601.000111. Cerquetti M, degli Atti ML, Cardines R, Salmaso S, Renna G, Mastrantonio P. Invasive Type e Haemophilus influenzae Disease in Italy. Emerg Infect Dis. 2003;9(2):258-261. https://doi.org/10.3201/eid0902.020142.