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lab9 . Relationship of O lab9 . Relationship of O

lab9 . Relationship of O - PowerPoint Presentation

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lab9 . Relationship of O - PPT Presentation

2 to Bacterial growth Microorganisms divided due to their sensitivities to O 2 into Aerobic bacteria the bacteria that grow in the presence of oxygen Obligate or strict anaerobes can grow only in the absence of O ID: 914325

agar anaerobic water bacteria anaerobic agar bacteria water chamber grow jar microorganisms presence gaspak anaerobes fig cystine sodium dextrose

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Slide1

lab9

. Relationship of O

2

to Bacterial growth

Microorganisms divided due to their sensitivities to O

2

into:

Aerobic bacteria

the bacteria that grow in the presence of oxygen

Obligate or strict anaerobes

can grow only in the absence of O

2

and are harmed by its presence.

Facultative anaerobes

can grow either aerobically or anaerobically (in the presence or in the

abscence

of O

2

)

Aerotolerant

anaerobes

are microorganisms that cannot use O

2

,

Microaerophiclic

microorganisms

, that require small amounts of O

2

, but are inhibited at normal atmospheric tension of O

2

Capnophilic

bacteria

the bacteria that need 5-10%

CO

2

. see (fig. 20).

Slide2

Figure (20) Appearance of various agar deep culture

Slide3

The principles of Anaerobic Jar use

There are another ways (method) for cultivation or culturing of anaerobic bacteria one of them is the

Gaspak

Anaerobic system (fig. 26).

In this system, hydrogen and CO

2

are generated by

Gaspak

envelope after addition of water .A palladium catalyst (pellets) in the chamber lid catalyzes the formed H

2

with O

2

to form water.

 

Palladium Catalyst

2H

2

+ O

2

--------------→ H

2

O

By this reaction quantity of O2 in the chamber is completely reduced. Therefore the environment inside the chamber becomes anaerobically and convenient for the grow of the anaerobic microorganisms. Sometimes instead of

Gaspak

(H

2

and CO

2

) generator it can be used a flamed wax candle to reduce the O

2

inside the Jar chamber. The Anaerobic jar must be tightly closed.

-The inoculated agar plates incubate at 37°c for 24-48 hours.

- Observe the isolated colonies record the results in your report.

Slide4

Slide5

Slide6

Figure (

21)

Anaerobic cultivation in anaerobic jar

Slide7

The anaerobic culture media

Eugon

Agar (PH 7.0).

Tryptose

15.0

gm

;

soytone

5.0

gm

; Dextrose 5.0

gm

; L-

cystine

0.2

gm

; Sodium Chloride 4.0

gm

;sodium sulfite 0.2

gm

;Agar 15.0

gm;Distilled

water 1 liter.

Thioglycollate

broth (PH 7.0)and agar.

Peptone 15.0

gm

; yeast extract 5.0

gm

; Dextrose 5.0

gm;L-cystine

0.75

gm

;

thioglycolate

0.5

gm

;

NaCL

2.5

gm;Methylenc

or

Resazurin

0.001

gm

; Dist. Water 1.0 L.

15

gm

agar added for

thioglycollate

agar.

Isolation of Anaerobic bacteria from the soil

Or Dental specimens (infected root canal or plaque

etc

).