PDF-Elongation & Termination of Protein Synthesis (5.1)

Lecture 5 1 INITIATION l Assembly of active ribosome by placing the first mRNA codon AUG or START codon near the P site and pairing it with initiation tRNA fMettRNAfMetl Occurs o. 1 Lecture 5 1 INITIATION Assembly of active ribosome by placing the first mRNA codon AUG or START codon near the P site and pairing it with initiation tRNA fMettRNA fMet Occurs once per polypeptide chain or molecule 2 ELONGATION Involves three disti GO TO . BIOINTERACTIVE WEBSITE. DNA replication questions. Why is DNA replication so important?. In what part of the cell cycle does DNA replication occur?. What enzyme brings in the new DNA nucleotides that form complimentary pairs with the old DNA strand?. DNA .  RNA  Protein. 2 Major Steps. Transcription. – DNA is transcribed (copied!) into single stranded mRNA . (DNA code transcribed into RNA code). Translation. – mRNA is translated into protein (amino acids). genotype to phenotype. Look deep into nature, and then you will understand everything better.. Albert Einstein. . one-gene, one-enzyme hypothesis. Many enzymes are composed of more than one polypeptide chain, or subunit (that is, they have a quaternary structure). In this case, each polypeptide chain is specified by its own separate gene. Thus, it is more correct to speak of a one-gene, one-polypeptide relationship: The function of a gene is to control the production of a single, specific polypeptide.. Chapters 16 and 17. Before the end of the semester we will be covering…. Historical DNA experiments. Structure of DNA/RNA. DNA Replication. Protein Synthesis (Transcription and Translation). Mutations. Protein Synthesis. Protein Synthesis. DNA contains . genes. sequences . of nucleotide bases. These Genes code for . polypeptides (proteins). Proteins. are. used . to build cells and do much of the work inside cells. Learning Outcomes. At the end of this . topic . you should be able to . . Outline the steps in protein synthesis. Know that . DNA contains the code for protein. Know . that enzymes unwind the DNA. Know . Chapters 16 and 17. Before the end of the semester we will be covering…. Historical DNA experiments. Structure of DNA/RNA. DNA Replication. Protein Synthesis (Transcription and Translation). Mutations. TRANSLATION . Initiation, Elongation, and Termination of Protein Synthesis in Eukaryotes. Initiation:. Initiation . of protein synthesis differs significantly between prokaryotes and eukaryotes. Eukaryotic mRNA has no ribosome-binding site (RBS). Instead recognition and binding to the ribosome . The function of the different types of RNA in the cell is to facilitate the task of synthesizing proteins.. An activated . tRNA. with anticodon AGU bonds to serine at the acceptor stem.. Learning Goal . Look deep into nature, and then you will understand everything better.. Albert Einstein. . one-gene, one-enzyme hypothesis. Many enzymes are composed of more than one polypeptide chain, or subunit (that is, they have a quaternary structure). In this case, each polypeptide chain is specified by its own separate gene. Thus, it is more correct to speak of a one-gene, one-polypeptide relationship: The function of a gene is to control the production of a single, specific polypeptide.. 69NB. Directions:. Using the DNA strand as a template, transcribe mRNA. Make sure to use the correct Nitrogen bases.. ATA CCT TAA CGC GTC. TAT TAG GCA AAA TTC. GTG TGA TTA ATA GCC. CTA AAG GAA TAG GAT. Dr. . Manishi. . Tripathi. REVERSION. SUPPRESSION. . Informational . suppressors . Interaction . suppressors. Overproduction suppressors. Bypass suppressors. .. Physiological suppressors. What organelle makes proteins?. Where are these organelles found?. How does the information from DNA get to those organelles?. Protein Synthesis Overview. The information in . DNA . is used to make .