Rezić Andrea 1 Safner Toni 2 Buzan Elena 3 Potušek Sandra 3 Šprem Nikica 1 INTRODUCTION Alpine chamois Rr rupicapra habitat NATURA 2000 sites ID: 788552
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Slide1
Can the river act as genetic barrier for Alpine chamois population?
Rezić
Andrea1, Safner Toni2, Buzan Elena3, Potušek Sandra3, Šprem Nikica1
Slide2INTRODUCTIONAlpine chamois (R.r. rupicapra
) habitatNATURA 2000 sitesCroatia: 37% of the land territory and 15 % of sea shoreSlovenia: 37% of the country
Alpine chamois
Balkan
chamois
Hybrids
Slide3Scientific
papers
Slide4INTRODUCTIONThe Kupa river: administrative boundaryHypothesis: is
it boundary to gene flow?(does it prevent gene flow between chamois
subpopulations)
Slide5MATERIAL AND METHODS
STUDY AREA
CroatiaUp to 725m a.s.l.
Slovenia
Up to 876m
a.s.l
.
Kupa
river
Up
to 225m
a.s.l
.
Slide6MATERIAL AND METHODS STUDY AREA
Border security fence
2015.
Slide7MATERIAL AND METHODS
Slide8MATERIAL AND METHODS DATA ANALYSIS
54 individuals:17 SLO – population size 180
37 CRO – population size 110 20 STR lociDescriptive analysis of loci (Fstat software): - number of alleles - observed and expected heterozygosity - FIS
Slide9MATERIAL AND METHODS DATA ANALYSIS
Analysis of genetic structure - Geneland:1)
Non spatial model2) Spatial model with uncertainty on coordinates10 independent runs1000000 (million) iterations1000 thinningUncorrelated allele frequencies
Slide10RESULTS
Number of alleles
H exp.H obs.
FIS
p (10000 permutation)
BM1258
8
0,712
0,611
0,142
0,054
BOBT24
8
0,799
0,722
0,097
0,105
CSSM66
5
0,614
0,444
0,278
0,005
ETH10
6
0,755
0,778
-0,031
0,727
ETH225
3
0,563
0,574
-0,02
0,6295
ILST03
12
0,847
0,778
0,082
0,0945
INRA12
2
0,471
0,481
-0,023
0,677
MAF214
9
0,779
0,6670,1450,029NRAMP130,3420,1290,6230,0005OarFCB60,7250,630,1320,067OarFCB30,5220,593-0,1370,8935SRCR-Sp070,7580,5930,220,0035SRCRSP06120,8300,7410,1090,053SRCRSP09150,8370,5000,4050,0005SRCRSP1180,6860,6480,0550,299SY25950,7020,5930,1570,046SY43420,2140,1670,2220,1415SY5860,6760,5740,1520,0565SY8470,5190,4260,1810,0345TGLA5380,7410,7220,0260,422
Total FIS
0,1330,0005
H
exp
. 0.7117
H obs. 0.6111
Slide11RESULTS
Slide1220-month periodDirect and indirect deathsHigher impact in the initial periodbit.ly/2lQDRnW
Slo-Cro
Ungulates
30
Red deer
18
Roe deer
12
RESULTS
Slide13CONCLUSIONSRiver
Kupa is not a barrier for a chamois…Results indicate that population is stable.Border
security fence – future???
Slide14THANK YOU FOR YOUR ATTENTION!14
ACKNOWLEDGEMENTS
DNA as a evidence of distribution and vitality of endangered Balkan chamois – BalkCham IP-2016-06-5751