PDF-Serum Proteins on 2-D Gels

BioRad Expression Proteomics Enhance Your 2D Results Sample purification with the Aurum serum proteinmini kit ensures effective removal of albumin andIgG The removal of these major proteins reduces. Is there something fishy about evolution?. Bio-Rad Biotechnology . Explorer Comparative Proteomics Kit I: Protein Profiler Module. Instructors - Bio-Rad Curriculum and Training Specialists. Sherri Andrews, Ph.D., Eastern US. Y. e Zhang. B. ing . Xu’s. Group. Self-Walking Gel. Videos of Self-oscillating Gels. Malonic. Acid . (MA). BrO. 3. -. Ru(bpy). 3. HNO. 3. /H. 2. SO. 4. H. 2. O. Oxidant. Catalyst. Gel. Build in. oscillation. dodecyl. sulfate – . polyacrylamide. gel electrophoresis . (SDS-PAGE). Provides a means to look at all the proteins in a cell simultaneously. Electrophoretic mobility of a protein depends on its molecular weight. Total Protein . Concentration. 224 PHL. Lab#. 2. . 1) Simple Proteins:. . They yield only . α. -amino acids . on hydrolysis.. e.g. . albumin, globulin. .. 2) Conjugated Proteins:. . They are proteins combined with a non-protein substance called prosthetic group. . Protein Electrophoresis . (SPEP). Lab 3. What does it mean ?. Serum protein electrophoresis is an easy method of separating serum proteins based on their net charge, size, and shape. . A small amount of serum is placed on a specific medium (such as agarose) and an electrical charge is applied. . INTRODUCTION. Electrophoresis is a migration of a charged particle in a medium under the influence of an applied electric field.. The usual purposes of carrying out . electrophoretic. experiments are :. INDEX. Intoduction to culture media.. Components of serum.. Advantages and disadvantages of serum media.. Advantages and disadvantages of serum free media.. CULTURE MEDIA = defined as growth media suitable for the in- vitro cell culturing of human or animal cells and the composition of components are known.. preparations that contain. . small inorganic. particles or . large organic molecules. interpenetrated by a liquid.. The vehicle may be: Aqueous ,hydroalcoholic , alcohol based or non aqueous. In a typical polar gel, a natural or synthetic polymer builds a three-dimensional matrix throughout a hydrophilic liquid.. that contain small inorganic particles or large organic molecules interpenetrated by a liquid.. The vehicle may be. : . Aqueous. ,. hydroalcoholic. , alcohol . based . or non aqueous. In a typical polar gel, a natural or synthetic polymer builds a three-dimensional matrix throughout a hydrophilic liquid.. - D DIGE Huadong Liu Product Specialist Huadong.liu@ge.com GE Healthcare Life Sciences China 2 / GE DIGE / 3/22/2012 Agenda 1. 2 - D DIGE concepts and benefits 2. Biomarkers in colorectal cancer 3. adopt an extended rod-like conformation, . single-stranded DNAs and RNAs . often self-base-pair into complicated secondary structures. . As such, they migrate in gels un-predictive of their molecular weights, unless denaturants are added to resolve their correct size. Electrophoresis is a migration of a charged particle in a medium under the influence of an applied electric field.. The usual purposes of carrying out . electrophoretic. experiments are :. 1. To determine the number, amount and mobility of components in a given sample or to separate them.. . TOPIC: GEL ELECTROPHORESIS. Gel Electrophoresis. Gel electrophoresis is a method that separates macromolecules, either nucleic acid or protein.. Electrophoresis describes the migration of charged particles under the influence of an electric field.. 224 PHL. Lab#. 1. . Introduction . Instrument . used in Colorimetric . method: . Spectrophotometer. Blood. Urine. Cerebrospinal fluid (CSF). Saliva. Types of Specimens used in the laboratory experiments:.