antibody to porcine corticotropin per- - PDF document

antibody to porcine corticotropin per- the accuracy corticotropin cell, to correlate cell types chemical staining. made on females treated with were given NaCl solution as drinking cortisol ace- NaCl solution. hypophyses were fluid, embedded on a One slide prepared for chemical with ten taken from gland. Single sec- tions obtained from each were placed cedure. This indirect method first, application antirabbit gamma- conjugated with peroxidase; dase. 3,3’-Diaminobenzidine substrates for the subsequently stained with pyronin. porcine corticotropin, sified with Freund’s This anti- low antibody titer. However, be used for the as little method involves steps, numerous tropin cells was obtained the fol- to hormone Nakane-Pierce pro- normal rabbit serum to egg albumen for immunoreactive sites on the hormone when attached to the corticotropin cell unconjugated anti-rabbit with conjugated anti- the staining following observa- corticotropin cells anti-PC was previously with the (presumably containing hypophyses obtained filtration through a 89.5 cm monium bicarbonate, brated with a reference proteins the concentration absorption. Absorption which should have contained only buffer did not interfere corticotropin cells was obtained anti-PC was previously corticotropin Refer- On the other identical concentrations with bovine (NIH-LH-B-I, bovine, or folli- cle-stimulating hormone neutralize the the antiserum these hor- mones. The anti-PC tended more background incubation in and treatment osmic acid activated charcoal. osmic acid and when a-naphthol was as the substrate for the immunochemical corticotropin cells, double- carried out corticotropin cell or prolactin this purpose (anti-HGH) and were used observations were similarly the relation corticotropin cell cell by cell types were revealed a single section. cell types revealed several procedures were fol- First, after the selected cells were and then fuchsin and the Masson a-amylase before immunochemically, destained, antibody re- and then a chemical sections were imm~lnochemically and The cell type revealed corticotropin cell, consti- the female cell cord sinusoids. Long proc- esses commonly from the end on a sinusoidal wall; occasion- one cell formed a bridge between two sinusoids. processes occasionally cells were ior border, where growth the superior medial region where basophils abundant. The pars intermedia stained with intensity which was usual- ly above the depth intermedia rarely approached single sections showed tropin cell growth hor- any reactivity anti-PC. However, addi- tional study to prove anti-HTSH used. corticotropin cell than that for other basophil category. Light with varying corticotropin cells been previously stained im- However, aldehyde cells (fig. immunochemical procedure, stained immunochemical- cells to some cells gave weak positive always less than that amylase digestion. Prior immunochemi- seemed to cells (fig. corticotropin cell histochemical detection. Corticotropin cells physiological conditions. They to be intensely stained compared with Ovariectomy elicited cell; most cytoplasm were mildly duced. Seven some corticotropin cells were three-day postoperative little alteration was observed. in the male cells were hypertrophied seven although they somewhat less intense- with dexamethasone days induced a variable reduction corticotropin cells. However, with cortisol a longer a profound reduction and staining corticotropin cells workers to corticotropin cell understandable. After fixation, the with light manner. The corticotropin cells with reasonable on the to pull the plasmalemma. the smaller The capacity fuchsin and procedure indicates muco- or glycoprotein be classed as a Such a conclusion would agree with the workers previously cited who employed immunofluorescent proce- reaction with under normal circumstances mucoprotein accumulate within the cell significant degree. Since immunoglobulins prior application may have enhanced the fuchsin and PAS. How- acid dis- sociation did corticotropin cells to these dyes. Likewise, nor prolactin respectively, followed conjugate. The acidophil because cell identified corticotropin cell this study been classified most earlier seems reasonably ticotropin cells conclusion is based she attributed adrenalectomy cell: gland, general distribution, shape with cytoplasmic processes neighboring cells. Siperstein cell which may account the present authors have staining the corticotropin cell adrenalectomy cell chromophobe. Her subsequent demonstration with electron cytoplasm suggests be considered general, the corticotropin cell varied directly the smaller correlated with a probable lower evidence. Using bioassay procedure less corticotropin the male testosterone seems to testosterone therapy prevents the pituitary corticotropin cells more active the female therefore, be this study. ectomy does not alter the amount the gland (Kitay, corticotropin cells. studies dealing with the been performed on male these investigations Kitay, '63) reveal significant rise the seventh marked enlargement tropin cells observed be expected. Similar- ly, Hess reaction with fluorescein-labeled antibody to corticotropin cells Data pertaining ectomy on rats is less extensive. postoperative period. modest hypertrophy and magnitude than that profound reduction corticotropin cells days correlates with the reduced corticotropin tural changes probably be The results out indicate the demonstration fact that pituitary fractions corticotropin, negated effectiveness while absorption non-hormonal protein hormones failed to is the with melanocyte-stimulating hormone (MSH) common core identical amino acid sequence. concerning the distribution pars inter- Cross-reaction between pars intermedia stained more intensely the pars distalis. Hess reported significant pars intermedia p1-a4-corticotropin. Additional producing these hormones, extensive experimental data. First, crine alteration and that may be positive cell type the same basophils. Second, the ultrastructure corticotropin cells what similar, respect to differentiation hazardous with this ment (Herlant granules about with smaller particulates (Hymer The successful delinea- with the Nakane- lends strong remains uncertain philic cell types tropin cell. conclusion, the addition to a unique the female the male, sponds predictably to alteration in the corticotropin. Although and irregular procedure suggests needed before corticotropin cell basophil cells been identified previously LITERATURE CITED R. Midgley, growth hor- peroxidase-labeled antibody. adrenotropic substance hypophysis as 41: 1117-1125. by age, parathyroidectomy. Anat. Quantitative studies hypophysis following corticosteroids following bilateral adrenal- the rat. the rat. Biol. Med., C. A. following adrenalecto- my. Endocrinology, von p1-24-Corticotropin. Virch. Two types the anterior pituitary spective cytophysiological significance. Endo- Rev. Cytol., R. Acad. Sci. pituitary. Experientia, pituitary granules and the gonadal hor- mone replacement. Immunohistologische Befunde Ratte. Naturwissenschaften, mal and multiple tissue antigens using antibody method: study on pituitary the rat. Histochem. Cytochem., the lieht and pituitary luteinizing hormone. Biol. Chem., cells responsible intact and tropin cell; areas illustrated were located anterior to sections were peroxidase-labeled antibody. subsequently with which accounts for ground staining. Corticotropin cells are intensely stained, Corticotropin cells Cytoplasmic processes sinusoid. Note some cells. hypophysis seven days after compared with normal male after treatment with acetate for Corticotropin cells Corticotropin cells as the peroxidase substrate. Thyrotropin stained immunochemically, with a-naph- peroxidase substrate. Female. Corticotropin cell showing wall. Although general cytoplasm stained, darker at the stained with PITUITARY CORTICOTROPIN CELL B. L. Baker, S. Pek, A. R. Midgley, Jr. and B. E. Gersten PLATE 1 565 stained immunochemically corticotropin cells was un- Corticotropin cells were stained immunochemically with was stained with hematoxylin. The corticotropin cells here lightly contain fine corticotropin cells were stained immunochemically, and then a more corticotropin cells than that typically more phils other corticotropin cells PITUITARY CORTICOTROPIN