19 ZWITTERIONIC NONDETERGENT SULFOBETAINESNondetergent SulfobetainesAlthough not detergentsthese reagents possess hydrophilic groups similar tothose of zwitterionic detergents but with much shorter ID: 253862
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19 Physical Propertiesis the critical micellular concentration,the concentration at which micelles begin to form (i.e.the maximummonomer concentration).It should be noted that micelles cannot form,even above this concentration if the tempera-ture is too low.The minimum temperature for self-aggregation is called the critical micellar temperature (CMT).Thelower the CMC,the more stable the micelle and the more slowly molecules are incorporated into or removed from themicelle.CMC values are a guide to detergent hydrophobic binding strengths.The higher the CMC,the weaker the bind-ing and the easier the removal of the detergent,such as by dialysis.Aggregation Number is the average number of monomers in a micelle.A low aggregation number and highCMC favor removal by dialysis.is the hydrophile-lipophile balance.It deÞnes the hydrophilic character of the detergent.A low HLB favorsremoval of the detergent by reverse-phase chromatography.Cloud point temperature is the temperature at which a detergent solution begins to look cloudy due to aggregationinto larger structures that scatter light.The cloud point phenomenon interferes with applications that require opticalclarity,but can be used to advantage in removing a detergent from aqueous solution.Cloud Point temperature is the temperature at which a detergent solution begins to look cloudy due to aggre- ZWITTERIONIC NON-DETERGENT SULFOBETAINESNon-detergent SulfobetainesAlthough not detergents,these reagents possess hydrophilic groups similar tothose of zwitterionic detergents but with much shorter hydrophobic chains.They may improve the yield of membraneproteins when used with detergents and reportedly prevent aggregation of denatured proteins.Brij 58 P Detergents Properties and Applications Product Code(s)B 951814840P 1254P 588429467293959619329396D 7658D 4641H 62629420630727O 8001 30726O 6004D-1414010840223I 7771O 3129N 1138D 627774385P 1300S 452117228T 8532X-114P 8942P 1504P 8192 I 30217438893427P 8341P 6474 P 7949P 8074 MW (anhydrous)878862120011224394534955094835115674974992923363081229218246*3213363506808350*5836255371228*1310 CMC** (mM)3.41.1-1.40.05-0.10.083401202.4-50.561.60.150.00060.590.920-250.994.60.5680.085819-256-7.0590.04*0.10.2-0.90.20.060.0270.012 Aggregation Number108-1620-4070**6663*98***84**601312232********100-155***60 HLB **16.915.7***************13****29**13.512.416.7*15 Cloud Point (¡C)**�100�100�100*********45-50***652376*65 Average Micellar Weight8,80010,50048,00079,000**32,60032,000*50,000***25,000**70,00028,597549,965 Diagnostic Applications¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥ ¥¥¥¥ ¥¥¥¥¥¥¥ ¥¥¥¥¥ ¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥ Enzymology¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥¥ Antigen/ Vaccine ¥¥¥¥ Drug Delivery/Liposomes¥¥¥¥¥¥¥¥¥¥¥¥ SPECIFICATIONS Sodium cholateSodium deoxycholateSodium glycocholateSodium taurocholateSodium taurodeoxycholateLithium dodecyl sulfateSodium dodecyl sulfateammonium bromide(CTAB)Trimethyl(tetradecyl)ammonium bromide (TTAB) CATIONIC SPECIFICATIONS APPLICATIONS A 134694508C 0856C 9426C 955145165O 6626D 4266D 4516T 7763H 6883O 80048280417236 216667-08-252562-29-5*75621-03-382473-24-366455-29-615178-76-415163-36-714933-08-514933-09-62281-11-013177-41-815471-17-7081239 435463400615631272280308336364392420201257 ***681.6-2.133025-402-40.1-0.40.01-0.06*These products do not ***1011**415583155*form micelles ************ ***�10090******* ***61507000**12,60018,50030,20060,700* ¥¥¥ ¥¥¥¥ ¥¥¥¥¥¥¥¥¥¥¥¥¥¥ ¥¥¥¥¥ ¥¥¥ Product Code(s)C 1254D 6750G 7132T 4009T 0875L 7414L 4632L 3771H 6269T 4762 L 9150H 5882 CAS Number73163-53-8302-95-4863-57-0145-42-61180-95-6137-16-62044-56-6151-21-357-09-01119-97-7 CMC** (mM) 9-152-673-111-414.67-107-1014-5 Aggregation Number2-33-122462*6217080 HLB 1816*****40** Cloud Point (¡C)�100** Average Micellar Weight900-13001200-5000100021003100600*18,00062,00027,000 Diagnostic Applications¥¥¥¥¥¥¥ Molecular Biology¥¥¥¥ Cell Culture Chromatography¥¥¥¥ Solubilization¥¥ Enzymology¥ Antigen/ Vaccine Preparation¥¥¥¥¥¥ Drug Delivery/ Liposomes¥¥*data not available**CMC in water at 20-25 ¡C