UCP3 Liver Muscle UCP3 SDH p lt005 p lt005 p lt005 p 005 p lt005 p lt005 a b p lt001 p lt005 Figure S5 A Contribution of ANT to proton leak in liver mitochondria from WT and Grx2 mice Following a measure ID: 635392
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Slide1
Supplemental Figure 5
d
c
+ - + - UCP3
Liver
Muscle
UCP3
SDH
p
<0.05
p
<0.05
p
<0.05
p
=0.05
p
<0.05
p
<0.05
a
b
p
<0.01
p
<0.05
Figure S5
:
A)
Contribution of ANT to proton leak in liver mitochondria from WT and Grx2-/- mice. Following a measure of state 3 respiration, mitochondria were sequentially treated with
oligomycin
(
Oligo
;
2.5
μ
g/mL),
carboxyatractyloside
(CAT;
5
μM
), and
antimycin
A (
4
μM
). Oxygen consumption rate (OCR) values were corrected for background (
antimycin
A) respiration. n=4,
mean±SEM
, 2-way ANOVA with Fisher’s
posthoc
test.
B)
Contribution of ANT to proton leak in muscle mitochondria from WT and Grx2-/- mice. Following a measure of state 3 respiration, mitochondria were sequentially treated with
oligomycin
(
Oligo
;
2.5
μ
g/mL),
carboxyatractyloside
(CAT;
5
μM
), and
antimycin
A (
4
μM
). OCR values were corrected for respiration not associated with the electron transport chain (
antimycin
A).
C)
Immunoblot
analysis of UCP3 protein levels in mitochondria collected from WT and UCP3-/- mice.
D)
Contribution of ANT to proton leak in muscle mitochondria from WT and UCP3-/- mice. Following a measure of state 3 respiration, mitochondria were sequentially treated with
oligomycin
(
Oligo
;
2.5
μ
g/mL),
carboxyatractyloside
(CAT;
5
μM
), and
antimycin
A (
4
μM
). OCR values were corrected for respiration not associated with the electron transport chain (
antimycin
A)Student
t
-test, n=4,
mean±SEM
.