PPT-Balloon-based mutagenesis & protein folding

Deborah L Crittenden Why proteins Molecular machinery of life eg ATP synthase enzyme dualaction molecular motor ion pump chemical factory The protein structure problem Human proteome . Muhammad Shoaib Amjad. 11-Arid-3758. PhD Botany. WHY PROTEIN FOLDING?. Most proteins must fold into defined three-dimensional structures to gain functional activity. . polypeptide chain -Immediate folding amplification Proteinsmediate virtually all cellular functions •Cellular structure •Transport across membranes –Import of nutrients –Export of The classic principle of . protein folding. is that all the information required for a protein to adopt the correct three-dimensional conformation is provided by its amino acid sequence.. Molecular chaperones. Archaea. Matthew Blow. mjblow@lbl.gov. Adam . Deutschbauer. Morgan Price. Kelly Wetmore. Adam . Arkin. Cindi. Hoover. Feng. Chen. Jim Bristow. Deutschbauer. lab, LBNL. JGI. G. ene function annotation . Chris Garlock. Protein Folding - Why is it important. Proteins are biological nano-machines which play apart in all of our bodies functions. Protein folding is the process all proteins undergo to assemble into their native structure. Stolen and Edited by: Keith King. Objectives . Review central dogma of molecular biology. . Discuss type of protein.. Assess amino acids.. Demonstrate folding proteins.. protein folding video. Review – Central Dogma of Molecular Biology . Advisor: Dr. Chen . Keasar. Arie Barsky, Nadav Nuni. Protein folding problem. Proteins are responsible for constructing and operating the organism, and are made of chains of amino-acids. Protein folding problem. Liwo, 1,~ Jarostaw Pillardy, 2 Cezary Czaplewski, 1,~ Jooyoung Lee, 2 Daniel R. Ripoll, s Malgorzata Groth, 1 Sylwia Rodziewicz-Motowidlo, 1 Kajmund Ka~.mierkiewicz, 1 Kyszard J. Wawak, 2 Stanislaw Ot DISEASES.  . How . Proteins . fold and why they . misfold. Role of Molecular Chaperones in Protein Folding . ORGANELLE-SPECIFIC PROTEIN QUALITY CONTROL SYSTEMS AND PROTEIN MISFOLDING DISEASES. Mechanisms and Link to Disease . Mechanism of folding and . misfolding. GroEL. – biological machine (chaperones folding). Molecular motors: Polymer physics and Myosin V motility. Many Facets of Folding . Structure Prediction. Protein & Enzyme Design. 1 : hsp104, 90, 70, 60 and small hsps, including homologues of lens -crystallin.Catalysts of folding : Protein disulfide isomerase, Peptidyl prolyl cis-trans isomeraseNucleoplasmin : nucleosomeassembl The ‘Native State’ structures look like this:. But how did they get there (Kinetics) and why do they stay that way (Thermodynamics)?. We’ll start at the very beginning: Primary structure. Protein Folding: The Early Years…. Proteins – Now that Everything has Changed. The Model Lending Library. At the MSOE Schroeder Library. A collaborative project of the MSOE Center for . BioMolecular. Modeling (CBM), the MSOE Walter Schroeder Library and 3D Molecular Designs (3DMD) that makes many of the physical models produced by the CBM and 3DMD freely available for short-term loan.. This has proved to be a very challenging problem. It has aptly been described as the second half of the genetic code, and as the three-dimensional code, as opposed to the one-dimensional code involved in nucleotide/amino acid sequence. .