PPT-RNAseq When things go wrong

something always goes wrong John Hutchinson Harvard Chan Bioinformatics Core RNAseq workflow design experiment isolate samples isolate RNA ribodeplete make libraries and barcode samples sequence. risk. Gianni . Baroncini. Valerio Meloni. 1. Wrong way . risk. Definition. Examples. Regulation. Measuring. Management. 2. Wrong way . risk. This type of risk occurs when exposure to a counterparty is . Other . Religions. ?. Text: . I Jn. 4:1. Scripture Reading: Mt. 15:7-14. Is It Wrong To Criticize Other Religions?. Intro . Some people get . offended at . criticism of other religions. It . is often an emotional plea of some kind, rather than a careful study of . . RNASeq. Pipeline. Steve Searle. Outline of Talk. What the . Ensembl. . RNASeq. pipeline does. Ensembl. Pipeline infrastructure. How we’ve modified the process as its scaled . up. Cloud version. Genome Browser. Combines a genome database with interactive web pages. Allows the user to retrieve and manipulate database record through a graphical user interface (GUI). Different types of information are displayed in an intuitive fashion in user-configurable “tracks”. Galaxy overview and Interface. Getting Data in Galaxy. Analyzing Data in Galaxy . Quality Control. Mapping Data. History and workflow. Galaxy Exercises . (https://. galaxy.bioinfo.ucr.edu. /). NGS Analysis Using Galaxy. Day 1: Library prep, gel purification: Ligate 3' Adapter: 1.5 hrs, waiting time 45 min; Ligate 5' Adapter: 1.5 hrs, waiting time 45 min; Reverse Transcription: 1h 15 min, waiting time 45 min; Gel puri ORFans. to . “GO”. Genomic sequence of model eukaryote . Saccharomyces cerevisiae . completed in 1996. (12.1 Mb). Despite 16 years of intense research, function of nearly 30% of putative open reading frames (ORFs) remain unknown.. Two Delivery Successes That Led to Product Failure. Two Delivery Successes That Led to Product Failure. Product X - Building the wrong thing. Product Y - Building the right thing at the wrong time. Disclaimers. . transcriptome. RNA-. seq. reads. Illumina. sequencing. mRNA. RNA-. Seq. Alignment. 35bp - 150bp. s. ingle or paired-end. reads. RNA-. seq. alignments. Wang K., et al., . MapSplice. : Accurate Mapping of RNA-. No Wrong Door System Vision. A visible and integrated system that empowers Hoosiers to make informed decisions about their long term services and supports needs. What is a No Wrong Door System?. A No Wrong Door (NWD) system: . Murder, theft, lying.. Redemptive Law. The God-given way to deal with failures. Changes from dispensation to dispensation. Leviticus. Ch. 1-7 Sacrificial Laws. Ch. 8-10 Consecration of the Priesthood. - Overview -. Why gene expression analysis?. Quantification of mRNA transcript abundance. High specificity, +/- high through-put. Requires sequence knowledge. Considerations. Experimental question. Species limitations . QC. 1. QC summary. 2. QC was performed on all 192 samples focusing on determining failed or outlier. samples. Four samples are recommended for omission from the final analysis. dataset based on evidence of RNA degradation, PCA analysis, and model-based. Vince Buonaccorsi. Juniata College. Cost of sequencing. https://www.genome.gov/about-genomics/fact-sheets/Sequencing-Human-Genome-cost. Next (2. nd. ) Generation Sequencing. How does Illumina sequencing work?.