PPT-RNAseq When things go wrong

something always goes wrong John Hutchinson Harvard Chan Bioinformatics Core RNAseq workflow design experiment isolate samples isolate RNA ribodeplete make libraries and barcode samples sequence. T wo things in particular stand out to me There is always one or two people who pro fess with great gusto that they are the final word on Christian doctrine They are convinced they have all truth but if anyone disagrees with what they say they openl What could possibly go wrong. 2) Anticipating, Preparing and Preventing . 3) When Things Go Really Wrong!. Stress accompanies a real estate sale for most everyone involved in the process. . There are so many steps to buying or selling and each step can hold an opportunity for a road block.. . RNASeq. Pipeline. Steve Searle. Outline of Talk. What the . Ensembl. . RNASeq. pipeline does. Ensembl. Pipeline infrastructure. How we’ve modified the process as its scaled . up. Cloud version. 17%21%21%25%31%28%39%40%8%9%7%11%9%7%75%77%70%62%54%55%57%53%0%20%30%40%50%60%70%80%90% Right Direction40%39%38%40%39%28%32%31%25%21%21%17%18% Unsure15%8%5%5%7%7%9%7%11%9%8%6%7% Wrong Track45%53%57%55 Genome Browser. Combines a genome database with interactive web pages. Allows the user to retrieve and manipulate database record through a graphical user interface (GUI). Different types of information are displayed in an intuitive fashion in user-configurable “tracks”. and . transcriptomics. Daniel Hurley. What are we going to talk about?. Understanding the core principles and ‘root hypothesis’ of . transcriptomics. Choosing between different . technologies. How to design an experiment. Day 1: Library prep, gel purification: Ligate 3' Adapter: 1.5 hrs, waiting time 45 min; Ligate 5' Adapter: 1.5 hrs, waiting time 45 min; Reverse Transcription: 1h 15 min, waiting time 45 min; Gel puri OR IRRELEVANT? . Matthew 5:1-16. The Effect of Moral Upheaval. To muddy the waters regarding what is morally right and wrong. To call into question the validity of traditional Judeo-Christian moral teachings. Especially – if really want a new one. 10 years old. Has 150,000 miles. Paint faded. Needs tires. Carpet has stains. A/C has leak. $28,000. Runs good. Good gas mileage. Paid for. Ample room. Can be fixed for very little. Mistakes happen when…. you don’t know the law, regulatory requirements or best practices.. Keep learning.. A licensee told a buyer that if she changed her mind . for any reason. after entering into a contract, she didn’t have to remove subject clauses and she would get her deposit back.. Murder, theft, lying.. Redemptive Law. The God-given way to deal with failures. Changes from dispensation to dispensation. Leviticus. Ch. 1-7 Sacrificial Laws. Ch. 8-10 Consecration of the Priesthood. Conjunctions. One of the features ASL shares with English is the use of conjunctions to join simple sentences. Many students can’t stop signing in English word order because they feel “wedded” to these conjunctions: . The bestselling book revealing why Americans are so fearful, and why we fear the wrong things--now updated for the age of TrumpIn the age of Trump, our society is defined by fear. Indeed, three out of four Americans say they feel more fearful today than they did only a couple decades ago. But are we living in exceptionally perilous times? In his bestselling book The Culture of Fear, sociologist Barry Glassner demonstrates that it is our perception of danger that has increased, not the actual level of risk. Glassner exposes the people and organizations that manipulate our perceptions and profit from our fears: politicians who win elections by heightening concerns about crime and drug use even as rates for both are declining advocacy groups that raise money by exaggerating the prevalence of particular diseases TV shows that create a new scare every week to garner ratings. Glassner spells out the prices we pay for social panics: the huge sums of money that go to waste on unnecessary programs and products as well as time and energy spent worrying about our fears.All the while, we are distracted from the true threats, from climate change to worsening inequality. In this updated edition of a modern classic, Glassner examines the current panics over vaccination and political correctness and reveals why Donald Trump\'s fearmongering is so dangerously effective. It’s no secret that this world we live in can be pretty stressful sometimes. If you find yourself feeling out-of-sorts, pick up a book.According to a recent study, reading can significantly reduce stress levels. In as little as six minutes, you can reduce your stress levels by 68%.