Learning Objectives Educators attending this workshop will Utilize tools that support NGSS student learning outcomes of three dimensional lesson design including Molymods to model basic amino acid structure and build a dipeptide ID: 703295
Download Presentation The PPT/PDF document "5 E’sy Ways to Investigate enzymes!" is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
Slide1
5 E’sy Ways to Investigate enzymes!Slide2
Learning Objectives
Educators attending this workshop will:
Utilize tools that support NGSS student learning outcomes of three dimensional lesson design including -
Molymods
to model basic amino acid structure and build a dipeptide
Amino Acid Starter Kit
©
to simulate protein folding
Enzymes in Action
©
to demonstrate enzymatic specificity and actions
Acetylcholinesterase Active Site Cube
©
to apply knowledge of protein structure/function to an observed environmental phenomenon
Landscapes of David
Goodsell
©
to apply multiple representations to a concept
Learn something new and interesting for your own professional development.
H
A
V
E
F
U
N
!Slide3
Learning Objectives
What NGSS dimensions will be targeted?
SEPs
CCCs
DCIs
Asking questions
Patterns
PS1.A: Structure and properties of matter
Developing and using models
Cause and effect
PS1.B: Chemical reactions
Constructing explanations
Scale, proportion, and quantity
LS1.A: Structure and function
Structure and function
LS2.C: Ecosystem dynamics, functioning, and resilienceSlide4
the Mosquito
Protein Structure versus . . .
Engage!
https://www.orkin.com/other/mosquitoes/
https://cdn.rcsb.org/pdb101/learn/resources/what-is-a-protein/what-is-a-protein.pdfSlide5
Amino Acids:
Building Blocks for Proteins
Explore!
Explain!Slide6
Amino Acids:
D and L Forms of Amino Acids
Extend!
http://www.phschool.com/science/biology_place/biocoach/bioprop/landd.htmlSlide7
Building a DipeptideSlide8
The Protein Folding Problem
Beware student preconceptions about protein folding!
Amino acid #1
Amino acid #2
http://www.biochem.ucl.ac.uk/~martin/c40/peptide.htmlSlide9
Modeling Protein Structure
Elaborate!Slide10
Modeling the Primary Structure
2 cysteine side chains (
green
).
2 acidic side chains (
red
).
2 basic side chains (
blue
).
Your favorite 3 hydrophilic amino acids (white).
Your favorite 6 hydrophobic amino acids (
yellow
).
Randomly distribute them on the TOOBER. (Space them about three inches apart to get an even distribution).
You will need 15 total side chains:Slide11
Modeling Tertiary Structure
Fold your protein according to basic principles of chemistry and physics:
In an aqueous environment, where do you expect the
hydrophobic
side chains to fold? Why?
Likewise, consider where the
hydrophilic
side chains would fold.
What interaction may occur between
acidic
and
basic
amino acids?
What interaction may occur between
cysteine
amino acids?Slide12
Modeling Secondary Structure
Beta Sheets
Alpha Helices
Why
are
secondary structures important to protein structure/function?Slide13
Modeling Quaternary Structure
HemoglobinSlide14
Modeling Enzyme Action: Catabolism
Extend!
https://www.khanacademy.org/science/biology/energy-and-enzymes/introduction-to-enzymes/a/activation-energySlide15
Modeling Enzyme Action: Anabolism
Extend!Slide16
Modeling Enzyme Action: Specificity
Extend!Slide17
Competitive Inhibition
Noncompetitive Inhibition
Modeling Inhibition
Extend!Slide18
the Mosquito
Protein Structure versus . . .
Evaluate!
https://www.orkin.com/other/mosquitoes/
https://cdn.rcsb.org/pdb101/learn/resources/what-is-a-protein/what-is-a-protein.pdfSlide19
Neuromuscular Signaling
A Landscape from David
Goodsell
https://www.education.com/Slide20
A Molecular Story:
Acetylcholinesterase!Slide21
Resources!
http://www.rcsb.orgSlide22
http://pdb101.rcsb.org/
Resources!Slide23
THANK YOU!
For more molecular stories. . .
Come visit us at booths 419 and 421!
We hope to see you soon!Slide24
Another Molecular Story:
The Beery Twins!Slide25
ENZYMES!A key enzyme in the Beery twins molecular story is
sepiapterin
reductase
.
Sepiapterin
reductase is necessary to make a cofactor called
tetrahydrobiopterin
.
What’s going on with the Beery twins?Slide26
What’s going on with the Beery twins
sepiapterin
reductase?Slide27
Joe Beery’s Contribution:
A single amino acid change!
Arginine
Glycine
What’s going on with the Beery twins
sepiapterin
reductase?Slide28
Retta Beery’s Contribution:
A missing piece of the terminal end of the protein.
What’s going on with the Beery twins
sepiapterin
reductase?Slide29
Analyzing Data:
The
Beery’s
Pedigree
How did the Beery twins get the variant forms?Slide30
Joe
Beery’s
Mutation – Missense at 498
DNA base change from adenine to guanine
Retta Beery’s Mutation – Nonsense at 801
DNA base change from adenine to thymine resulting in an early stop codon.
What are the implications of these variant forms?Slide31
The Beery Twins Today! Slide32
Resources!
http://www.raregenomics.org/e-books/Slide33
THANK YOU!
For more molecular stories. . .
Come visit us at booths 419 and 421!
We hope to see you soon!