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Summer research safety training Summer research safety training

Summer research safety training - PowerPoint Presentation

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Summer research safety training - PPT Presentation

  900      Welcome overview of lab safety check lists inventory assignment other Pam RiggsGelasco 1000    Chemical Hygiene Plan requirements Randy Beaver 1100    Overview of ID: 759819

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Slide1

Summer research safety training

 9:00      Welcome, overview of lab safety check lists, inventory assignment, other, Pam Riggs-Gelasco10:00    Chemical Hygiene Plan requirements, Randy Beaver11:00    Overview of ChemSW live and inventory control, Meredith Jenkinson11:30    Departmental safety Presentation, part 1, Jim Deavor12:30    Lunch- provided by the department1:30      Fire extinguisher safety behind building, coordinated by Jeff Tomlinson2:30      Departmental Safety Presentation, part 2, and overview of safety and operations checklist process, Jim Deavor

1

Slide2

THE CHEMISTRY LABORATORYINCLUDES HAZARDS AND RISKS.

This presentation summarizes some of the safety rules for a research laboratory. If some of the material is unfamiliar, or you did not take general and organic chemistry at the College of Charleston, you should review the information in the attached link: General Chemistry Lab Safety

2

Slide3

Laboratory Safety

3

Slide4

Personal Protective Equipment: What must be worn when you work in the laboratory.

4

1. PPE

Eye ProtectionLab CoatLong PantsClosed Toed Shoes – no exposed skin around feetLab gloves – when required

Slide5

Eye Protection

Contact lenses are OK as long as glasses/goggles are wornPrescription glasses – you must wear goggles over themSafety goggles are provided in organic labs in UV irradiating cabinetsEye wash stations are present in all labs

5

Slide6

Clothing must cover all exposed skin including legs/ankles

Stockings or leggings do not provide good coverageSandals, flip-flops, Crocs, open-toe and open-top (i.e. ballet flat) shoes and canvas shoes (i.e. Toms) are not appropriate. These are not going to protect your feet if you drop a piece of glass with a liquid chemical reagent in it.

Clothing and Foot Protection

6

Slide7

Result of Improper Footwear in a Laboratory

Your PI will send you home to change if you do not

have appropriate shoes or other required PPE.

Northwestern University, Evanston, IL July 2003

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Slide8

Wear gloves of a material known to be resistant to permeation by the substances in use – nitrile is good for most of our laboratory classes.

Inspect each glove for small holes or tears before use. When you spill on your glove or tear it, change it immediately. Throw gloves away any time you take them off.

Hand Protection: Chemically resistant Lab Gloves

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Slide9

Karen Wetterhahn (October 16, 1948 – June 8, 1997)

The latex gloves she was wearing were not resistant to methyl mercury – it passed through the glove, through her skin, entered her blood system and resulted in her death weeks after the exposure.

Dartmouth College

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Slide10

Use of Gloves

Remove gloves before handling objects such as doorknobs, telephones, pens, computer keyboards, pH meter or other electronic buttons, or phones while in lab. It might be convenient to have one gloved hand and one ungloved hand to do procedures where these kinds of things are used.Throw away gloves anytime you take them off.You should expect to use several pairs of gloves in any given lab period.

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Slide11

Tie back long hair before entering the laboratory, don’t wear dangling jewelry.

Yale physics student Michele

Dufault

was killed in a shop accident in April 2011 that would have been prevented had she tied her hair back

11

Slide12

UCLA Lab Fire: December 29, 2008

Sheri Sangji was using this plastic syringe to transfer tert-butyllithium. This was not the correct procedure, because this compound is well-known to ignite if it is comes in contact with air. The syringe plunger dropped out of the syringe and the reagent ignited. Sheri died January 16, 2009 of severe burns. She was wearing nitrile gloves but no lab coat. The students assisting her did not remember to put her under the safety shower.

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Slide13

Lessons from UCLA accident

Lessons: Know the proper procedures for transferring dangerous reagents. Wear your lab coat at all times in the lab. Know where safety shower and other emergency equipment is – you may need to be the one who needs to be ready to act when your lab mate is unable to help himself/herself.

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Slide14

2.Eyewash and Safety Shower: Know where these are in your lab.

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Slide15

Eyewash / Safety Shower

The eyewash is on the left. Pull the handle and a fountain of water will appear that you can use to bathe your eyes.

The

safety shower

is on the right. Pull the handle and water will start spraying from the shower head on the ceiling. There’s no drain in the floor – we only do this in emergencies, because a flood of water will have to be cleaned up.

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Slide16

16

Eye Wash

Slide17

Safety Shower

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Slide18

3.Chemical Fume Hoods: You must do your experiment in the hood if any of your reagents are flammable, have harmful fumes or present a splash or explosion hazard.

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Slide19

Using the Fume Hoods properly

If this is not saying NORMAL, then the hood

is not protecting you. Keeping the sash

and sliding panels in proper position keeps thisNORMAL, otherwise the alarm goes off. If the alarm goes off, you need to reposition things to the correct positions, then press the “mute” button to reset the controller.

The sash should never be raised abovethe green “operation” level when youare working in the hood.

This

window/bar

is called

the sash.

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Slide20

×

Closed, not in use

In use, side-to-side panel

used as shield

In use, sash (window) raised

to less than 18 inches

Don’t open side shields

to make one big window.

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Slide21

When using a laboratory hood, Check that the airflow is in the normal range on the digital displayTurn on the hood lightSet the equipment and chemicals back at least 6 inches.Never lean in and/or put your head in the hood when you are working. This is worse than doing the experiment with no hood at all.It’s a good idea to put liquid reagent containers in trays to catch all spills and drips

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Slide22

4.Know the risks of the chemical reagents you are working with

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Slide23

Labels are important

Even if it seems obvious.

In the chemistry lab, nothing is ever obvious.

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Slide24

NFPA Diamond

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Slide25

MSDS (SDS)

Provides procedures for handling or working with that substance in a safe manner

Includes physical data: melting point, boiling point, flash point, etc. Includes safety data: incompatibilities, toxicity, health effects, reactivity, storage, disposal: protective equipment & spill-handling procedures first aid

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Slide26

How to find an MSDS (SDS)

There are on-line repositories of MSDS that can be searched by the following methods:Common NameIUPAC (International Union of Pure and Applied Chemistry) NameC.A.S. NUMBER – (Chemical Abstracts Service) a number assigned to all commercialized chemicals available in the USThe easiest to use is the CAS number, as it is a unique identifier that isn’t subject to spelling errors

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Slide27

Incompatible materials

Certain chemicals should not be stored and cannot be safely mixed with certain other chemicals due to severe reaction exotherm or uncontrolled production of a toxic product.

27

Every lab has a

legible matrix that lists the general classes of materials that should not be mixed together:

Slide28

Texas City Disaster of 1947

Incompatible oxidizer and fuel source mixed Worst industrial accident in American historyFreighter full of ammonium nitrate and fuel oil (ANFO) exploded, igniting other ships581 dead, entire city devastated

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Ship anchor thrown across city by explosion

Slide29

Introductory toxicology

AN MSDS contains valuable information on the health dangers of the chemicals but often use concepts and acronyms that are new to students:

29

PEL

TLV

LD50 and LC50

CAS #

STEL

Right to Know acts

TWA

OSHA

TOSCA

NIOSH

PROP 65

RCRA

Mutagenicity vs. teratogenicity

You need to know what is what to read an MSDS

IDLH

Slide30

Regulatory agencies and standards

Over the last 40 years the US and state governments and various international bodies have developed regulations and standards that try to improve safety and industrial hygiene standards including the following:EPA: Environmental Protection Agency, who have the primary responsibility to ensure chemicals are used and disposed of in an environmentally sensitive mannerTOSCA: the Toxic Substances Control Act of 1976 regulates which chemicals may be produced or imported in the USOSHA: Occupational Safety and Health Administration is the US agency that assures safe and healthful working conditions by setting and enforcing standards NIOSH: National Institute for Occupational Safety and Health is responsible for researching the prevention of work-related injury and illness, and providing guidance to OSHARCRA: the resource conservation and recovery act of 1976 that sets the standards for chemical waste disposal in this country and oversees the “superfund law” CERCLACalifornia Proposition 65: The state of California passed a very rigorous law to protect drinking and ground water from toxic chemicals. It is increasingly the standard for companies when evaluating chemical safety

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All of these regulations have been developed to make the use and handling of chemical safer, so their impact on lab safety has been profound

Slide31

Acute and chronic toxicology

Acute toxin: rapid absorption of the substance and the exposure is sudden and severe. Normally, a single large exposure is involved. Examples are carbon monoxide, hydrofluoric acid, hydrogen cyanide and nicotine Chronic toxin: prolonged or repeated exposures of a duration measured in days, months or years. Symptoms may not be immediately apparent.Examples of chemicals of high chronic toxicity include dimethylmercury, nickel carbonyl, benzo-a-pyrene, N-nitrosodiethylamine, and other human carcinogens or substances with high carcinogenic potency in animals

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Slide32

Carcinogens, mutagens and teratogens

One of the most significant chronic risks associated with chemicals is their potential to cause cell mutation and proliferation. Carcinogen: chemicals that can increase the incidence of cancer in the bodyMutagen: chemicals that cause mutations in DNA that lead to hereditary genetic defects in a fetusThere are two other general classifications that you should be aware of:Teratogen: chemicals that induce non-hereditary malformations of a fetusSensitizer: chemicals that no reaction in a person during initial exposures, but further exposures will cause an allergic response to the chemical

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Slide33

Routes of Entry and Allowable Exposure Limits

There are four main routes by which hazardous chemicals enter the body:Inhalation: Absorption through the respiratory tract. Most important in terms of severity.Skin absorption.Ingestion: Absorption through the digestive tract. Can occur through eating or smoking with contaminated hands or in contaminated work areas.Injection. Can occur by accidental needle stick or puncture of skin with a sharp object.Most exposure standards, Threshold Limit Values (TLVs) and Permissible Exposure Limits(PELs), are based on the inhalation route of exposure. expressed in terms of either parts per million (ppm) or milligrams per cubic meter (mg/m3) concentration in air.Other measures of chemical exposure:Lethal dose or concentration for 50% of the exposed population (LD50 or LC50) expressed in mg contaminant per kg of body weightShort term exposure limit (STEL or TLV-STEL) is the amount of a substance you can be exposed to for 15 minutes four times a day

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Slide34

5.Fire Safety

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Slide35

Fire Alarms – know the location of one close to your lab

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Slide36

Fire Extinguishers – we have several in the labs and in the hallways.

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Slide37

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Slide38

Types of Fire Extinguishers

Most of our fire extinguishers are

ABC

.

It contains a dry powder to put out the kinds of fires we might encounter in the chemistry labs where we have class.

This is a special fire extinguisher for combustible metal fires. It is a type

D fire extinguisher. You won’t need to use this unless you work in a research lab with combustible metals.

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Slide39

Student Reaction in a Fire

Although we want you to be informed on the operation of a fire extinguisher, we do not expect you to use it. If a fire is ignited in your area, the proper STUDENT response is to:Notify everyone in the roomIf possible shutdown any reaction in progress by removing heat/energy source and/or pulling plug on power cordProceed to the nearest exit and pull the nearest fire alarmEvacuate the buildingAssemble in front of the library or in the YWCA parking lot for a positive headcount

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Slide40

Flammables, combustibles, and potentially explosive materials

There are different ways of designating that a chemical is a fire risk:Flashpoint - minimum temperature at which a liquid gives off a vapor in sufficient concentration to ignite in the presence of an ignition sourceCombustible liquid - Any liquid having a flashpoint at or above 37.8oC (100 F) but below 93.3oC (200 F)Flammable liquid - any liquid having a flashpoint below 37.8oCAutoignition temperature - the lowest temperature at which it will spontaneously ignite in a normal atmosphere without an external source of ignition, such as a flame or sparkExplosive - A chemical that causes a sudden, almost instantaneous release of pressure, gas, and heat temperature.

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Synthesis of explosive material

In organic lab we often do reactions to add functional groups to benzene rings. A simple reaction is shown below:If a student overcharged the amount nitric acid and overheats the reaction the following chemical is isolated: Another risk is the formation of Peroxides – Explosives that can be generated in lab when organic ethers are heated for a prolonged period in the presence of air.

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Trinitrotoluene

If this is not handled carefully there are serious repercussions:

Slide42

Working with flames

Never leave experiments unattended unless you take special precautions to avoid accidents and you notify the responsible individuals. Flames are never allowed when flammable gases or liquids are in use.Always alert others before lighting a flame.Never leave a flame unattended under any circumstances.Turn off the natural gas at the valve when you are finished with your workIn the organic lab, Bunsen burners are rarely used to either (1) to pull TLC spotting tubes; or (2) to conduct flame or combustion tests.

Closed valve is

Perpendicular to hose

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Slide43

6.Gas Cylinder Safety

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Slide44

Gas Cylinders

http://www.youtube.com/watch?v=mReuQCuJNQQA gas cylinder will become a missile if the valve is broken or cracked.For this reason, gas cylinders must always be securely chained to a wall or a permanent bench in the lab. The chain should not be loose.If a cylinder is not in use or is going to be moved, it must be capped to protect you and everyone else in the building.Do not attempt to move a gas cylinder until you have been trained on this important procedure.

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Slide45

Gas Cylinder Safety

regulator

Do not

attempt to adjust

valves on regulators

×

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Slide46

7.Disposal Procedures

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Slide47

Broken Glassware

Always check your glassware and discard any with chips, breaks, or obvious flaws.Throw away broken glassware into special glass waste containers, NOT the trash.Do not overfill broken glass boxes, replace when ¾ full

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NO

YES

Slide48

Waste Disposal

Waste containers are provided for chemical waste generated in laboratoriesSome things can go down the sink, some can’t. Always check with your PI.Care must be used to avoid mixing incompatible chemicals such as Acids with BasesOxidizers and FlammablesWater reactive and aqueous solutionsCyanides and acids

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Slide49

University of MarylandSeptember 26, 2011

Students were conducting an experiment with nitric acid and sulfuric acid was added into a chemical waste container, causing a violent chemical reaction sparked a small fire in and near the laboratory chemical ventilation hood. Two female students were injured as a resultSustained first- and second-degree chemical burns and superficial cuts.

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Slide50

Handling Waste in Labs

Organic liquids like CH2Cl2 (aka methylene chloride, dichloromethane) & acetone, & TBME & liquid reagents PUT IN ORGANIC (HALOGENATED) LIQUID WASTE CONTAINER IN WASTE HOODAqueous – neutral (not basic or acidic) containing trace organics PUT IN AQUEOUS WASTE CONTAINER IN WASTE HOODAqueous – neutral (not basic or acidic) containing NONTOXIC salts with no trace organics CAN GO IN PUBLIC SEWAR, DOWN THE DRAIN (Use the “Would I want to swim it rule?”. Yes? Then put it down the drain. NO? Then put it in the aqueous waste container.)Solid chemical – old products, left over starting materials, includes organic and inorganic PUT IN SOLID WASTE CONTAINER IN WASTE HOODSolid, non toxic waste (paper towels, notebook pages) PUT IN TRASH ONLY IF SAFE TO TOUCH WITH BARE HANDS

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Slide51

Think First, Dispose Second

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Paper, without Chemical

Residue

Clean Broken Glass

Chemical Waste

Paper, without Chemical

Residue

Clean Broken Glass

Chemical Waste

Paper, without Chemical

Residue

Clean Broken Glass

Chemical Waste

Slide52

8.How to be a good lab citizen

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Slide53

Be prepared before walking into the lab. Think about the how and why before doing anything. Begin with a clean, neat work area; make it so. Minimize clutter; store book bags, equipment, etc. Have instructions, pen and notebook available. Return materials and equipment to proper places. Make it clean and neat and orderly before leaving.

SEVEN must-have habits for lab-work

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Slide54

Keep your lab area clean.

×

×

×

×

Throw away used paper towels and used gloves,

immediately.

Don

t block the floor in front of the eyewash/shower station.

Don’t leave things in the floor because someone will trip over it.

Don’t leave cords dangling because someone will trip over them.

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Slide55

Take care not to ingest anything in the laboratory!Food, gum, beverages, candy, and tobacco products are never allowed in the laboratory.Don’t apply makeup, chap-stick, lotion, or anything to your face or hands during lab. Wash your hands with soap then leave the lab before touching your face or other exposed skin.

Don’t put anything on your face or in your mouth while you’re in lab.

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×

Slide56

Don’t use any distracting electronic devices while in laboratory. If you touch your phone during lab, you’re contaminating it with whatever chemicals you’ve been working with.Do not wear earbuds in the lab. You need to be able to hear important announcements, especially in an emergency or when a safety concern is addressed.

Stay aware of what’s happening around you while you’re working in the lab.

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×

Slide57

Chemical storage

Flammables/combustiblesAcidsBasesOxidizersNonreactive (e.g., brine)

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Slide58

Mineral Acids – inorganic acids

Strong Mineral Acids

Hydrochloric AcidHydrobromic AcidHydroiodic AcidNitric AcidPerchloric AcidSulfuric Acid

Weak Mineral Acids

Phosphoric AcidBoric AcidHydrofluoric Acid

Mineral Acids can be stored together except for Nitric Acid, which must be stored by itself because it is also strong oxidizer.

Slide59

Storing Acids and Bases

Mineral Acids can be stored in the same cabinet as Bases, as long as they are physically isolated from each other.

If your lab contains some of each of these categories of acids, you should have the following separate cabinets:

Mineral Acids + Bases

Organic Acids

Nitric Acid (Strong Oxidizer)

Slide60

Nitric Acid

If nitric acid is mixed with a flammable organic compound, such as acetic acid, the heat from the oxidation and neutralization reactions is enough to ignite the flammable material. Nitric acid also slowly destroys its red plastic bottle cap. Always replace with a new red cap. Nitric acid may turn yellow over time because of the release of nitrogen dioxide on exposure to light. The yellow color does not affect the product’s usefulness in the school laboratory.

Slide61

Sulfuric Acid

Concentrated sulfuric acid is a strong dehydrating agent. Because of its strong ability to remove water, it reacts violently with many organic materials such as sugar, wood, and paper. If sulfuric acid has turned brown, it has probably been contaminated with an organic material and its purity should be in question.

Slide62

Hydrochloric Acid

Concentrated hydrochloric acid fumes continuously and cannot be stored without releasing hydrochloric acid vapor. These fumes are responsible for most of the corrosion damage in your chemical storeroom. Storing hydrochloric acid in a wood or plastic-lined acid cabinet is a must. Hydrochloric acid fumes will quickly corrode metal cabinets.Hydrochloric acid fumes mixed with ammonia fumes will react to form ammonium chloride clouds and possibly toxic chloramines. Open containers of these two reagents should not be in the same hood.

Slide63

HF

By definition a weak acidHowever - highly corrosiveMajor health hazardabsorbed quickly though the skin severe systemic toxinbinds Ca in the blood, bones & other organs & causes damage to tissues that is very painful & exposure to 2% of body to conc. HF can be lethaldamage can continue for several days after exposure

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Slide64

Chemical Spills

Notify your PI and your neighbors if you spill chemicals on the floor or bench.Don’t try to clean it up yourself. Your PI may need to use a specially designed chemical spill kit.http://www.youtube.com/watch?v=Dtp9vT15qIs

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9.Procedures and Practices

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Slide66

Texas Tech January 7, 2010

Conducting research funded by the U.S. Department of Homeland Security on energetic / explosive compoundsAttempting to produce 100 times more of an explosive compound than the informal lab limit (100mg)Lesson: Follow instructionsin the lab.

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Process safety

When performing an experiment always consider the following:Is the material flammable, explosive, corrosive, or reactive?Is the material toxic, and if so, how exposure to the material occur What kind of personal protective equipment or ventilation is needed to protect myself?Will the process generate other toxic compounds, or could it result in a fire, explosion, etc.?Are storage facilities appropriate for the type of materials used? Can incompatible materials be properly segregated?What possible accidents can occur and what steps can be taken to minimize the likelihood and impact of an accident?What are the proper procedures for disposal of the chemical(s)?As an example of process safety consider distillation:

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Slide68

What I Don’t Know Can’t Hurt Me

At a school far, far away; at a time long, long ago; there was an organic student performing a reaction that required heat. So the student, happily and cheerfully set about to heat the solution. The procedure stressed the need to prevent loss of material due to boiling off of solvent during the heating process. The student did not read over all the details carefully, but did think to cap the top of the apparatus to prevent the solvent from boiling off. Pleased for having remembered that little detail, the student was shocked and mad when the instructor saw what had been done and asked, in a rather loud and obnoxious voice: “What the heck are you doing?” Look at the picture when it pops up and see if you can identify what it was that had the instructor so upset. When the student was told by the instructor what the problem was, the student’s response was (this is a true story): “Well I do not think that matters, what I don’t know can’t hurt me.” Trust us, you do not want to be that student.

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Slide69

The “Apparatus”

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If Heated, What Would Happen?

HINT: PV = n RT

Slide70

OSHA FACT SHEET

Laboratory Safety Chemical Hygiene Plan (CHP)OSHA’s Occupational Exposure to Hazardous Chemicals in Laboratories standard (29 CFR 1910.1450), referred to as the Laboratory standard, specifies the mandatory requirements of a Chemical Hygiene Plan (CHP) to protect laboratory workers from harm due to hazardous chemicals. The CHP is a written program stating the policies, procedures and responsibilities that protect workers (at CofC “workers” includes faculty, students and staff) from the health hazards associated with the hazardous chemicals used in that particular workplace.

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Slide71

CofC - CHP

Who wrote the CofC CHP?Director of Environmental Health & Safety (with input from faculty & staff)Where can you find the CofC CHP?In the labOn Departmental Web Site

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Slide72

GHS

Now that you’ve had an overview of the various factors that will help you be safe in a lab, we need to introduce the next generation proposals:GHS: the Global Harmonization SystemGHS is being incorporated by OSHA into the Hazards Communication Standard (HAZCOM) that ensures people who handle chemicals are properly trainedNew symbols for labels with universal usage are being developed:

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Slide73

Overview

OSHA has issued final rule to revise 29 CFR 1910.1200 (Hazard Communication Standard)

Details released 3/20/12, final rule to appear in 3/26/12

Fed. Reg

.

Goal is to integrate components of the UN project’s Global Harmonization Standard (GHS) into

HazCom

Rule modifies MSDS requirements, labeling, classifications, and requires retraining of all employees.

Significant opposition to standard from some business groups due to:

Inclusion of “unclassified hazards”- altered in final rule, but legal challenges possible

Some disputed economic impact estimates (costs of training, revised labels and MSDSs etc.)

Slide74

GHS Symbols

Slide75

Overview

Hazard classification

: Provides specific criteria for classification of health and physical hazards, as well as classification of mixtures.

Labels

: Chemical manufacturers and importers will be required to provide a label that includes a harmonized signal word, pictogram, and hazard statement for each hazard class and category. Precautionary statements must also be provided.

Safety Data Sheets

: Will now have a specified 16-section format.

Information and training

: The Final HCS will require that workers are trained within two years of the publication of the final rule to facilitate recognition and understanding of the new labels and safety data sheets.

Slide76

Physical Hazards

Slide77

77

Hazard ClassHazard CategoryAcute Toxicity1234Skin Corrosion/Irritation1A1B1C2Serious Eye Damage/ Eye Irritation12A2BRespiratory or Skin Sensitization1Germ Cell Mutagenicity1A1B2Carcinogenicity1A1B2Reproductive Toxicity1A1B2LactationSTOT –Single Exposure123STOT – Repeated Exposure12Aspiration1Simple AsphyxiantsSingle Category

Health Hazards

Slide78

Labeling

For labeling, manufacturer/importer must include:

Product identifier

Signal word

Hazard statement(s)

Pictogram(s)

Precautionary statement(s)

Name, address, and telephone number of responsible party

Once a chemical has been classified, the label preparer can obtain the relevant harmonized information from Appendix C

OSHA is maintaining the flexibility for workplace signs and labels.

Use of third party hazard rating systems such as the NFPA diamonds and HMIS is still a valid approach in the workplace

Slide79

Students must report any injuries, big or small.

Report all injuries to the PI. We will not call emergency services unless the PI determines it is a serious injury.An incident report will be filled out whether it is small or serious.

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Slide80

Injury procedure, continued

First Aid kits are available in the lab with band aids and other items for treating small cuts and burns.If it is a serious injury, your PI will call campus emergency services, 843-953-5611. Our campus officers will work with the PI and/or injured student to determine whether or not 911 EMS should be called in.

80

Slide81

843-953-5611

Please take a moment now to program this number into your cell phone.

Once again, the number to call in an emergency is:

81

Slide82

Report any concerns

If you have any safety concerns about the lab you are working in or the people working around you, you can contact:Your lab PIDr. Neal Tonks – Head of the departmental safety committeeDr. Pamela Riggs-Gelasco – Department Chair for Chemistry and BiochemistryDr. Jim Deavor, Associate Dean of the School of Science and Mathematics.

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