PPT-ENZYME-CATALYZED REACTIONS

Case of Polyphenoloxidase INTRODUCTION Browning Fruits and vegetables Enzymatic Nonenzymatic Oxidation of phenolic compounds Sensory properties Color Flavor Bad Good INTRODUCTION PPO. Chapter 2: Section 2.5. 1. Objectives. SWBAT explain the effect of a catalyst on activation energy. . SWBAT describe how enzymes regulate chemical reactions. . and maintain homeostasis.. 2. Starter: How can this be possible. • Be able to describe a the key properties of a metabolic pathway.. • Be able to describe the various modes of regulation of metabolic pathways as they are presented in this lecture.. • Be able to explain the key properties of coenzymes, and know the types of group carried by each coenzyme discussed in this lecture.. Objectives. To monitor the progress of an enzyme catalyzed reaction (Acid phosphatase).. To determine the initial rate of the reaction (. V. i. ).. Important terms and points:. Enzyme kinetics. The central approach for studying the mechanism of an enzyme-catalyzed reaction is to study enzyme kinetics. . Chemical Reactions. :. A . chemical reaction . is a process that changes one set of chemicals into another by rearranging chemical bonds. The elements you start with are . reactants. The elements you end with are . Part 1. . Chapter 3. Learning outcomes. You should be able to:. explain how enzymes work. describe and explain the factors that affect. enzyme activity. use V. max. and K. m. to compare the affinity of different enzymes for their substrates. Christian Kendall and Galen Mack-Crane. Reactions. Mass-Action Kinetics. Michaelis and Menten (1913). . First Order. Time (years). [C] conc. by %mass. Second Order Unimolecular. Enzyme-Substrate Reactions. In this experiment, we will continue to study acid phosphatase kinetics.. Objective:. To establish the relationship between enzyme concentration and the rate of an enzyme catalyzed reaction.. The reaction rate will increase as the concentration of enzymes is increased but there must be a . 322 BCH. . . Exp. (6). In this experiment, we will continue to study . acid phosphatase . kinetics.. Objectives. To establish the relationship between pH and the rate of an enzyme catalyzed reaction.. • Be able to describe the various modes of regulation of metabolic pathways as they are presented in this lecture.. • Be able to explain the key properties of coenzymes, and know the types of group carried by each coenzyme discussed in this lecture.. Regulation of enzymes Introduction andProperties of Enzymes Enzymes are characteristics to facilitate the Enzyme-catalyzed reactions take place Reactants need to pass over the energy barrier, Catalys .. The length of the transcription bubble is about 12 to 14 . bp. , but the length of the RNA–DNA hybrid within the bubble is only 8 to 9 . bp. . As the enzyme moves along the template, the DNA duplex reforms. -. The SSERC Team. Effect of competitive and non. –. competitive inhibitors on . . ‑galactosidase. Paul Beaumont / Kate Andrews /. Margaret Louis. b. -galactosidase. b. -galactosidase. Competitive inhibition. Drill:. . What background knowledge do you have on enzymes?. Enzyme Structure and Function. Enzymes are . protein catalysts. They speed up the rate at which reactions occur. Lower the activation energy by creating a microenvironment that is energetically more favorable for a reaction. enzymes. . . The . substance converted by an enzyme is called a . substrate. , and a substance resulting from the conversion of the substrate is called the . product. . . The . enzyme is not consumed during the reaction catalyzed by it, so one enzyme molecule is involved in the conversion of many substrate molecules .