PPT-PDZ Binding peptides

Characterisation of Neuroprotective PDZ binding peptides by Jamie AlNasir Project supervisor Prof Brian Austen Presented as part of MPharm project at Kingston university and St Georges Hospital medical school 24012011. Outline. Tandem Mass Spectrometry. De Novo Peptide Sequencing. Spectrum Graph . Protein Identification via Database Search. Identifying Post Translationally Modified Peptides . Spectral Convolution . Peptide Bond. Amino acid residues in peptides and proteins are linked together through a covalent bond called the peptide bond.. Two amino acid molecules can be covalently joined through a substituted amide linkage, termed a . Protein characterization: post-translational modifications and protein-protein interactions . (Week 10). Top down / bottom up. Top down. Bottom up. mass/charge. intensity. Top down Bottom up. exocytosis. and fission of artificial vesicles. E. Boenzli. 1. , M. Hadorn. 1. , D. de Lucrezia. 2. , M. G. Jørgensen. 3. , P. Eggenberger Hotz. 4. , M. M. Hanczyc. 1. , T. Yomo. 5. References. (1). CHAPTER 3, Part 1 . Amino Acids and Peptides . To know the structure and naming of all 20 protein amino acids. To know the structure and properties of peptides and the particularly the structure of the peptide bond.. omics. data integration. Michael Tress. CNIO. ,. Predictions: . Ensembl. /GENCODE . automatic . pipelines. , . HBM . and GENCODE RNA-. seq. . data, . individual large-scale studies. . Coding potential . Collagen. Most abundant insoluble fibrous protein in the connective tissue of mammals. Makes up to 25%-35% of the whole body protein content . It comes from the Greek work “. kolla. ” which means glue. It is also called glue-producer. exocytosis. and fission of artificial vesicles. E. Boenzli. 1. , M. Hadorn. 1. , D. de Lucrezia. 2. , M. G. Jørgensen. 3. , P. Eggenberger Hotz. 4. , M. M. Hanczyc. 1. , T. Yomo. 5. References. (1). h I g A h I gG h I gE h I g M m I gA m I gG m I gE m I gM B S A 0 0.05 0.1 0.15 0.2 T a r g e t p r o t e i n s A b s o r b a n c e a t 4 5 0 n m I n i t i a l l i b r a r y A f t e r 5 t h p a n n i A. PHYSICAL PROPERTIES. Optical properties: . All amino acids except glycine possess optical isomers due to the presence of asymmentric carbon atom. Some amino acids have a second assymmentric carbon e.g. isoleucine, threonine.. MacCoss. Lab. DIA Data Analysis Workflow. Peptide Selection. Chromatogram Extraction. Peak Integration. Transition Refinement. Hypothesis Generation. Hypothesis Test. Hypothesis Generation. Pathway. . Presentation. MHC-restricted antigen . recognition by . T. . cells. Any . T . cell can . recognize . an . antigen . on an APC only if . that antigen . is . displayed . by MHC. . molecules. Antigen . FIGURE 2. LC methodsA: Water 0.2% FAB: ACN 0.2% FA FIGURE 3. Peak widthPeak width 10-13 seconds9 scans across the peak0.100s scan time per transitionAll transitions summed per peptide FIGURE 1. Instru Assistant Professor. Department of Botany. BIOACTIVE PEPTIDES. CONTENT. INTRODUCTION. PROTEIN. PEPTIDES. BIOACTIVE PEPTIDES. PRODUCTION OF BIOACTIVE PEPTIDES. ENZYMZTIC DIGESTION. EFFECTS ON HEALTH AND RESOURCE.