Chelsea Zuania Keerthana Eduardo Transmission of hantavirus among reservoir host and humans Transmission of hantavirus Once the hantavirus has entered a species one of four events can occur ID: 927160
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Slide1
Hantavirus: Viral Evolution
Chelsea, Zuania, Keerthana, Eduardo
Slide2Transmission of hantavirus among reservoir host and humans
Slide3Transmission of hantavirus
Once the hantavirus has entered a species one of four events can occur:infect an epithelial cell
remain a free virus
eliminated by a natural killer cell
intercepted by an antigen presenting cell
Slide4Compartmental diagram of the activation of the immune system in response to an antigen
Slide5Replication of hantavirus
Subgenomicreplicates in parts rather than one piecehantavirus: tripartite (3 parts)
Largest strand
viral polymerase
Smallest strand
N, NS protein
Uses
nsRNA
genome as a cap snatching device
s
teals end of host mRNA genome
migration of newly formed
hantaviral
proteins
Slide6Deterministic Model (ODEs)
Slide7Conceptual Diagram
Slide8Stochastic Model (SDEs)
Adds Brownian motion
Slide9Slide10Slide11Slide12Quasispecies (viral evolution)
Each time the virus infects a cell and begins to replicate, changes in its genome occurNo phenotypic effectbehaves like the wild-type virusPhenotypic effect
new viruses to the antigen-specific immune responses
new CTL and antibodies specific to the new virus
Slide13Quasispecies (viral evolution)
Slide14Quasispecies Model
Slide15Hantavirus Structure
We used PlyMol
!
Crystal
structure of the N-terminal domain of the strain 1-75 nucleoprotein
Slide16Hantavirus Structure
PlyMol
again!
Crystal
structure of the N-terminal domain of the strain 76-118 nucleoprotein
Slide17Phylogenetic Tree
Slide18Selected References
L. J. S. Allen. An Introduction to Stochastic Processes with Applications to Biology. CRC Press/Chapman Hall, Boca Raton, FL, 2nd edition, 2010.G. Schonrich, A. Rang, N. Lutteke, M. J.
Raerty
, N.
Charbonnel
, and R. G. Ulrich. Hantavirus-induced immunity in rodent reservoirs and humans. Immun. Rev., 225:163{189, 2008.
Wordle
,
phylogenetic
data:
http
://www.ncbi.nlm.nih.gov
/
Slide19The End
Or is it