Rev 00 IINTRODUCTIONTrypticaseIIPERFORMANCE TEST PROCEDURE1Inoculate representative samples with the cultures listed belowaInoculate the tubes using sterile 10 mL pipettes with a dilution containing 1 ID: 884168
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1 TrypticaseSoy Broth with 5% Fildes Enric
TrypticaseSoy Broth with 5% Fildes Enrichment Rev. 00 IINTRODUCTIONTrypticaseIIPERFORMANCE TEST PROCEDURE1.Inoculate representative samples with the cultures listed below.a.Inoculate the tubes using sterile 1.0 mL pipettes with a dilution containing 1000 CFU/mL or less. For the TrypticaseTrypticaseb.Incubate tubes with loosened caps at 35 ± 2°C in an aerobic atmosphere supplemented with carbon dioxide.2.Examine tubes up to 7 days for growth.3.Expected ResultsOrganisms ATCCRecovery10211Growth 700344GrowthIIIADDITIONAL QUALITY CONTROL1.Examine tubes as described under Product Deterioration.2.Visually examine representative tubes to assure that any existing physical defects will not interfere with use.3.Incubate uninoculated representative tubes at 2025°C and 3035°C and examine after 7 days for microbial contamination.PRODUCT INFORMATIONIVINTENDED USETrypticaseVSUMMARY AND EXPLANATIONTrypticaseTrypticaseVIPRINCIPLES OF THE PROCEDUREenergy source. Sodium chloride maintains the osmotic equilibrium. Dibasic potassium phosphate acts as a buffer to control pH.VIIREAGENTSTrypticase Soy Broth with 5% Fildes EnrichmentApproximate Formula* Per Liter Purified WaterPancreatic Digest of Casein......................................17.0 gPapaic Digest of Soybean Meal..................................3.0 g Sodium Chloride..........................................................5.0 g Dextrose........................................................................2.5 g Fildes Enrichment......................................................50 mLWarnings and Precautions: Tubes with tight caps should be opened carefully to avoid injury due to breakage of glass. Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinicalOn receipt, store tubes in the dark at 28°C. Avoid freezing and overheating. Do not open until ready touse. Minimize exposure to light. Tubed media stored as labeled until just prior to use may be inoculated up to the expirationProduct Deterioration: L0094771of 2 VIIISPECIMEN COLLECTION AND HANDLINGdelivery to the laboratory. XIPROCEDUREMaterial Provided: TrypticaseMaterials Required But Not Provided:Test Procedure:Inoculate the medium as soon as possible after the specimens arrive
2 at the laboratory. Swab specimens may be
at the laboratory. Swab specimens may be inserted into theIncubate the tubes with loosened caps at 35 ± 2°C aerobically with or without supplementation with carbon dioxide. Tubedmedia intended for the cultivation of anaerobes should be incubated under anaerobic conditions. An efficient and easy way toUser Quality Control:XRESULTSXILIMITATIONS OF THE PROCEDUREXIIAVAILABILTYCat. No.DescriptionBBL TrypticaseBBL TrypticaseXIIIREFERENCES1.MacFaddin, J.F. 1985. Media for isolation-cultivation-identification-maintenance of medical bacteria, vol. I. Williams & Wilk2.Marshall, R.T. (ed.). 1993. Standard methods for the examination of dairy products, 16th ed. American Public Health Association, Washington,3.Forbes, B.A., D.F. Sahm, and A.S. Weissfeld. 2002. Bailey & Scotts diagnostic microbiology, 11th ed. Mosby, Inc., St. Louis.4. Fredette, V., and A. Forget. 1961. The sensitivity of several media to small inocula. Extract from a paper presented at the 5. Fildes, P. 1920. A new medium for the growth of . Br. J. Exp. Pathol. 6.National Committee for Clinical Laboratory Standards. 2001. Approved Guideline M29-A2. Protection of laboratory workers fromoccupationally acquired infections, 2nd ed. NCCLS, Wayne, PA.7.Garner, J.S. 1996. Hospital Infection Control Practices Advisory Committee, U.S. Department of Health and Human Services, Cen8.U.S. Department of Health and Human Services. 1999. Biosafety in microbiological and biomedical laboratories, HHS Publication (CDC), 4thed. U.S. Government Printing Office, Washington, D.C.9.Directive 2000/54/EC of the European Parliament and of the Council of 18 September 2000 on the protection of workers from ris). Official10.Murray, P.R., E.J. Baron, J.H. Jorgensen, M.A. Pfaller, and R. H. Yolken (ed.). 2003. Manual of clinical microbiology, 8th efor Microbiology, Washington, D.C.11.MacFaddin, J.F. 2000. Biochemical tests for identification of medical bacteria, 3rd ed. Lippincott Williams & Wilkins, Balti12.Holt, J.G., N.R. Krieg, P.H.A. Sneath, J.T. Staley, and S.T. Williams (ed.). 1994. Bergey's Manual of determinative bacteriology, 9th ed.ATCC is a trademark of the American Type Culture Collection.BD, BD Logo, BBL, GasPak and Trypticase are trademarks of Becton, Dickinson and Company. ©2006 BD. L0094772of 2