PDF-Proc.Nat.Acad.Sci.USAVol.71,No.4,pp.1133-1136,April1974TheEffectofRepl

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1134CellBiologyHalpernetalProcNatAcadSiUSA711974x900Co41UN600zin0030005010150TIMEhoursFIG1GrowthofW256ratbreastcarcinosarcomacellsincultureWalker256cellsweregrownintwomodifiedMcC

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Proc.Nat.Acad.Sci.USAVol.71,No.4,pp.1133-1136,April1974TheEffectofRepl: Transcript

1134CellBiologyHalpernetalProcNatAcadSiUSA711974x900Co41UN600zin0030005010150TIMEhoursFIG1GrowthofW256ratbreastcarcinosarcomacellsincultureWalker256cellsweregrownintwomodifiedMcC. Proc.Nati.Acad.Sci.USA87(1990)2527A4T4FIG.2.StereoscopicdifferenceFourierelectrondensitymapatthepositionofthemissingphosphateinbothA4andT4models.ofotherunlikelycombinationssuchasadodecamerduplexwithfo Proc.Natl.Acad.Sci.USA91(1994)5365emergeinearlyJune,becomeadultsinlateJune,andbegintodieoffinearlyAugust.Thegrasshoppernymphsandadultsareabundantduringthee80-daytemporalwindowwhenthekeyplantsareabunda Proc.Natl.Acad.Sci.USA88(1991)4529maximal(i.e.,50%)cellkilling.Chroniclineswereestab-lishedbymaintainingtheacutelyinfectedcellsuntilthecellssurvivingtheacuteinfectiongrewout.Experimentswiththechronica MedicalSciences:Cherif-Zaharetal.Proc.Natl.Acad.Sci.USA87(1990)6245aatcccggcctgcacagagacggacacaggATGAGCTCTAAGTACCCGCGGTCTGTCCGG60MetSerSerLysTyrProArgSerValArg9CGCTGCCTGCCCCTCTGGGCCCTAACACTGGAAGCAGCTC 868CellBiology:OsbornandWeberFIGS.1-7.(Legendappearsatbottomofthefollowingpage.)Proc.Nat.Acad.Sci.USA73(1976) Proc.Nat.Acad.Sci.USA73(1976)869FIG.8.Recoveryofcytoplasmicmicrotubulesin3T3cellsafterColc Proc.Nat.Acad.Sci.USA73(1976)951Themutagenicpotencyofeachchemicalisbasedonthere-versionofthattesterstrainmostsensitivetoit;eachstrainhasbeenindependentlyoptimizedfordetectionofapartic-ularclassofmutag Proc.Nat.Acad.Sci.USA70(1978)624~~~~~~~~~~~~24*0aS5!!402040608000020-tO60eo0as--40506~~~~~~~~~~~~~0GROWTHINTHEL00H000)CRW'0NTE0~Z04-20FIG.2.(A)Kineticsofinductionofthechloroplastisoleucyl-tRNAuponexpo Proc.Natl.Acad.Sci.USA93(1996)11529BothLandL*canbeprotonatedonLys-156,butthepKaforprotonationisconsiderablylowerforL*thanforL;pKL*iswhilepKLis10.OneimportanttenetofthismodelisthattheIndsmutationsincre 6148Biochemistry:LittleandHarperTable1.BacterialstrainsemployedSource,reference,StrainRelevantgenotypeorderivationAB1157recA+lexA+sup-37(15)DM49recA+lexA3sup-37(12)DM511recA+IexA3ts1-1sup-37(16)DM1187 I,fromFlavobacteriumokeanokoites,isamemberoftheunusual,typeIIsclassofrestrictionendonucleasesthatrec-ognizeaspecificDNAsequenceandcleavenonspecificallyashortdistanceawayfromthatsequence(1).Ibindstheco TheNunproteinofbacteriophageHK022isamemberofthearginine-richmotiffamilyofRNAbindingproteins,whichincludesthephageNtranscriptionantiterminationproteinandtheHIVTatandRevproteins(Fig.1;refs.1±3).Nunbind Proc.Natl.Acad.Sci.USA82(1985)6415fragmentwasisolatedfroma0.8%agarosegelbyusingtheapparatusforpreparativegelelectrophoresis.DNASequencing.DNAsequencingusingdideoxynucleo-tideswasperformedasdescribedby Proc.Natl.Acad.Sci.USA93(1996)2583multiplexamplificationasdescribedabove.Theonlydiffer-encebetweenthesetwoproceduresisthatsomePprimersusedinMGSmaycontainsingle-basemismatchestothetemplatestoconvertthe Proc.Natl.Acad.Sci.USA82(1985)ofcellsoveriodinevapors.Cellsthataccumulateglycogenstainbrown,whereasthosethatdon'tremainyellow.Quan-titativetrehaloseandglycogendeterminationsweremadeaccordingtoLilliean

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