TILED DATA RECONSTRUCTION AND CORRECTION - PowerPoint Presentation

TILED DATA RECONSTRUCTION AND CORRECTION
TILED DATA RECONSTRUCTION AND CORRECTION

TILED DATA RECONSTRUCTION AND CORRECTION - Description


ASHWINI NIKKAM JITHU JOSE SHRAVYA RAO 1 INTRODUCTION When a large biological specimen has to be pictured in high resolution it must be done in tiles  Tiles must be fit or stitched together to form one image  ID: 510927 Download Presentation

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stitching images bio data images stitching data bio plugin format olympus importer grid brightness conversion collection fiji image saves

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Slide1

TILED DATA RECONSTRUCTION AND CORRECTION

ASHWINI NIKKAM JITHU JOSE SHRAVYA RAO

1Slide2

INTRODUCTION

When a large biological specimen has to be pictured in high resolution, it must be done in tiles. Tiles must be fit or stitched together to form one image. Overlap  between each tile and its neighbors.

2Slide3

OUR GOAL

OLYMPUS Data ConversionAccurately stitch multiple imagesCorrect the brightness differences across the image

3Slide4

BIO-FORMAT IMPORTER

Bio-format Importer is a plugIn to load images into Fiji or ImageJ.

Opens proprietary formats that contains batches of images and converts into OME data model.

4Slide5

OLYMPUS DATA CONVERSION

Approach: A custom plugIn that calls the Bio-formats Importer PlugIn

The custom

plugIn

launches the Bio-format importer to select and read the images

Converts and saves images in .tiff format

5Slide6

OLYMPUS DATA CONVERSION PLUGIN

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OLYMPUS DATA CONVERSION

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STITCHING OF IMAGES

Stitching PlugIn is used to reconstruct big images from arbitrary number of tiled input images.Stitching has two plugIns

:

1.Pairwise stitching

2.Grid/Collection stitching

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STITCHING OF IMAGES

Pairwise stitching –Stitches, loads two images loads and saves to hard disk.Grid/collection stitching- Stitches an arbitrary collection or grid images(2d,3d,4d,5d) of same type .

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STITCHING OF IMAGES

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STITCHING OF IMAGES

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STITCHING OF IMAGES

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CORRECTING BRIGHTNESS

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CORRECTING BRIGHTNESS

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REFERENCES

http://fiji.sc/Bio-Formatshttp://fiji.sc/Image_Stitching

15Slide16

THANK YOU

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