TILED DATA RECONSTRUCTION AND CORRECTION - PowerPoint Presentation

Presentation on theme: "TILED DATA RECONSTRUCTION AND CORRECTION"— Presentation transcript

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TILED DATA RECONSTRUCTION AND CORRECTION

ASHWINI NIKKAM JITHU JOSE SHRAVYA RAO

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INTRODUCTION

When a large biological specimen has to be pictured in high resolution, it must be done in tiles. Tiles must be fit or stitched together to form one image. Overlap  between each tile and its neighbors.

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OUR GOAL

OLYMPUS Data ConversionAccurately stitch multiple imagesCorrect the brightness differences across the image

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BIO-FORMAT IMPORTER

Bio-format Importer is a plugIn to load images into Fiji or ImageJ.

Opens proprietary formats that contains batches of images and converts into OME data model.

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OLYMPUS DATA CONVERSION

Approach: A custom plugIn that calls the Bio-formats Importer PlugIn

The custom

plugIn

launches the Bio-format importer to select and read the images

Converts and saves images in .tiff format

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OLYMPUS DATA CONVERSION PLUGIN

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OLYMPUS DATA CONVERSION

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STITCHING OF IMAGES

Stitching PlugIn is used to reconstruct big images from arbitrary number of tiled input images.Stitching has two plugIns

:

1.Pairwise stitching

2.Grid/Collection stitching

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STITCHING OF IMAGES

Pairwise stitching –Stitches, loads two images loads and saves to hard disk.Grid/collection stitching- Stitches an arbitrary collection or grid images(2d,3d,4d,5d) of same type .

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STITCHING OF IMAGES

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STITCHING OF IMAGES

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STITCHING OF IMAGES

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CORRECTING BRIGHTNESS

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CORRECTING BRIGHTNESS

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REFERENCES

http://fiji.sc/Bio-Formatshttp://fiji.sc/Image_Stitching

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THANK YOU

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