PPT-Analyzing Proteins from a

Tyrannosaurus rex Todays Goal Learn how a mass spectrometer can be used to identify the types of protein in a biological sample Learn how proteins are analyzed from a dinosaur fossil Be able to analyze protein mass spectra and to compare two mass spectra in order to identify an unknown protein fragment. Introduction:. Plasma proteins are:. Serum albumin.. Serum globulin.. Fibrinogen.. Serum contains albumin and globulin.. Fibrinogen is absent . Converted into fibrin during blood clotting.. Therefore albumin and globulin are called serum albumin and serum globulin.. 2. Proteins. Proteins are polymers made of monomers called amino acids. All proteins are made of 20 different amino acids linked in different orders. Proteins are used to build cells, act as hormones & enzymes, and do much of the work in a cell. Protein. The . main function of protein is to . build and repair tissues . (muscle tissue). Protein. Protein can be a source of energy but should be used as a last resort. Importance, Characteristics, Structure, Classification, . Denaturation. Importance. Major structural components of animal tissues. Involved in the maintenance of life processes as communication (nerves), defense (antibodies), metabolic regulation (hormones), biochemical catalysis (enzymes), and oxygen transport (hemoglobin). CHAPTER 3, Part 1 . Amino Acids and Peptides . To know the structure and naming of all 20 protein amino acids. To know the structure and properties of peptides and the particularly the structure of the peptide bond.. Dialysis. (. Isolation Of Lactate Dehydrogenase Enzyme . ). BCH 333 [practical]. Objective:. 1-To . learn the technique of isolation of proteins on the basis of their solubility. . salting . in, salting out of proteins . Different types of . mb. proteins require different conditions for . mb. release. Integral membrane proteins directionally insert in the membrane bilayer. Outside cell. Inside cell. Glycophorin. and . Proteins account for more than 50% of the dry mass of most cells. Protein functions include structural support, storage, transport, cellular communications, movement, and defense against foreign substances. Unit objectives:. Identify amino acid classifications based on nutritional use and chemical properties of side chains. Describe the primary, secondary, tertiary and quaternary structures of proteins. Amino . Acids. In a transamination reaction, . aspartate transaminase (AST) . catalyzes the reversible transfer of an amino group between glutamate and aspartate.. an . α. -amino group is transferred from an amino acid to an . ANALYZING ARGUMENTS. Arguments can be analyzed, once recognized, by paraphrasing them or by . diagramming them. . . Paraphrasing . involves setting forth the argument in a clear and precise form. . Excercises. Angela Kohler. October 8, 2015. Analyzing Companies & Industries. Analyze the Industry First. : Provides Framework for the Estimates & Assumptions Used in Company Analysis. Top-Down Analysis. Allenoush . Hayrapetian. ahayrepe@iupui.edu . . Rajeev . Raje . rraje@iupui.edu. Computer Science Department. INDIANA UNIVERSITY-PURDUE UNIVERSITY INDIANAPOLIS. 10 February, 2018. Introduction. The goal of this research is to analyze the set of security requirements for any given software project and to provide feedback about its completeness and inherent ambiguity when evaluated with respect to a given security standard. . Each amino acid (aa) shares a common structure; i.e. an amine (NH. 2. ) group, an acid group (COOH), and a central carbon atom bonded to hydrogen and to a side chain (R).. The side chains qualify aas as acidic, basic, neutral, aromatic, and sulphur-containing amino acids..