PPT-Transcription Dr. Kevin Ahern

Structure Central Dogma Template Strand Coding Strand RNA Functions RNA Building Blocks Structure Eukaryotic Process RNA Polymerase Subunits Prokaryotes α 2 β β ω σ Transcription. and Its Regulation. January . 21 . –Mechanism of Transcription Initiation. January . 23– Regulation of of Transcription Initiation. January . 27–Mechanism and regulation of Transcription Elongation. DNA. RNA. PROTEIN. RNA and Transcription. RNA. R. ibo. n. ucleic . A. cid. another type of nucleic acid similar to DNA found in cells. also a polymer made of nucleotides. takes information from the nucleus to the rest of the cell. RNA Processing, and Transcriptional Regulation. Structure of RNA. Major Classes of RNA. Transcription in Prokaryotes. Transcription in Eukaryotes. Post-transcriptional Processing of Eukaryotic mRNA. Transcriptional Regulation in Prokaryotes:. Fat and Glycerophospholipid Synthesis. 1. 2. 3. 1. 2. 3. 1. 2. 3. Phosphatidic . Acid. Glycerol-3-P. Fat (Triacylglycerol). Glycerophospholipids. Fat Breakdown. Signaling. Catalysis. Glycero. Phospho. Nitrogen Metabolism. Nitrogen Forms in the Body. Nitrogen Balance Critical. Body Must Make and Break Down Amino Acids. Nitrogen Also Needed for Synthesis of. Nucleotides (ATP, GTP, CTP, UTP, dATP, dCTP, dGTP, dTTP). E S <=> ES <=> ES* <=> EP <=> E P. Michaelis-Menten Kinetics. E S <=> ES <=> ES* <=> EP <=> E P. Rate of Formation. Substrate. Product. Enzyme. Enzyme-. Part 1 - Amino Acids. Structure and Chemistry. Building Blocks of Proteins. Essential Amino Acids. Basic Structure. Stereochemistry. Side Chain Chemistry. Properties. Ionization. Bonding. Amino Acids & Proteins. synthesis). A. Prokaryotes: RNA polymerase, . Promoters – sigma factor. B. Eukaryotes: RNA polymerases, . Promoters – transcription factors. . 1 strand of . dsDNA. used as template to synthesize complimentary . Gene Expression. Gene Expression. Transcription. Splicing. Polyadenylation. mRNA Stability. Translation. Protein Stability. Controls on Protein Levels. Transcription Control - Prokaryotic Promoter. Transcription Control - Prokaryotic Promoter. Copyright 2016 by the Rector and Visitors of the University of VirginiaRNA nucleotides or building blocks are structurally very similar to DNA and go by similar names The only exception is that RNA ha th. Molecular . biology. According to . central dogma . , the genetic information flow from DNA the RNA via transcription process then it will translate later to functional protein. . Transcription . A eukaryotic transcription initiation complex consists of RNA polymerase II, . five. general transcription factors (GTFs), and a 20-subunit complex called Mediator (or . Srb. /Med). The CTD of RNA polymerase II anchors Mediator to the polymerase. Mediator allows RNA polymerase II to communicate with transcriptional . RNA Polymerases of Eukaryotes. Since typical eukaryotic cells have 10 times as many genes as do bacteria, the whole process of transcription and its regulation is more complex. . Indeed, eukaryotes have multiple RNA polymerases, unlike bacteria which have just one. Typical . Professional medical transcription services provided by SpectraScribe offer a streamlined solution for researchers seeking to extract meaningful insights from various research methodologies.