PPT-RNA Synthesis (Transcription)

Dr Shumaila Asim Lecture 2 1 RNA Synthesis Transcription Introduction First step in gene expression RNA is involved in the transfer of genetic information stored in DNA to proteins . 7. 7 RNA Synthesis and Processing. Chapter Outline. Transcription in Prokaryotes. Eukaryotic RNA Polymerases and General Transcription Factors. Regulation of Transcription in Eukaryotes. RNA Processing and Turnover. genotype to phenotype. Look deep into nature, and then you will understand everything better.. Albert Einstein. . one-gene, one-enzyme hypothesis. Many enzymes are composed of more than one polypeptide chain, or subunit (that is, they have a quaternary structure). In this case, each polypeptide chain is specified by its own separate gene. Thus, it is more correct to speak of a one-gene, one-polypeptide relationship: The function of a gene is to control the production of a single, specific polypeptide.. Replication. Functions (Stores and provides copies of genetic material- genes). Blueprint (genes) for Protein Synthesis (Enzymes and cell building blocks). Francis Crick. RNA (Ribonucleic Acid). Why do we need RNA?. Expression. MBIOL 6640. 10:45-11:35 ASB 210. Course director:. Dean Tantin, PhD. X7-3035, . dean.tantin@path.utah.edu. Lecture 0. For many of you this is a review of undergraduate material, but . study. Eukaryotic Cells – cells with a nucleus and many organelles. Organisms with nucleated cells belong to the largest classification group, Domain . Eukaryota. Four Kingdoms of Organisms which are eukaryotic are animal, plants, . synthesis). A. Prokaryotes: RNA polymerase, . Promoters – sigma factor. B. Eukaryotes: RNA polymerases, . Promoters – transcription factors. . 1 strand of . dsDNA. used as template to synthesize complimentary . Learning Outcomes. At the end of this . topic . you should be able to . . Outline the steps in protein synthesis. Know that . DNA contains the code for protein. Know . that enzymes unwind the DNA. Know . is a small molecule, existing as a single-strand that is . folded. into a . clover-leaf shape. .. tRNA. The role of tRNA is to bring the amino acids to a ribosome where protein synthesis is taking place. (more on this later). Chapter . 17.1-17.3. Important Terms to Know…. 25% of students confuse these terms in . FRQs!!. Replication. Copying of DNA . prior to cell division. (DNA .  DNA). Not prior to transcription. !. Central Dogma. The direction of the . flow of genetic information. is from DNA to RNA to polypeptide.. DNA Replication. Initiation and Unwinding of DNA. DNA . gyrase. . (. topoisomerase. ) unwinds the DNA coil.. .. The length of the transcription bubble is about 12 to 14 . bp. , but the length of the RNA–DNA hybrid within the bubble is only 8 to 9 . bp. . As the enzyme moves along the template, the DNA duplex reforms. Look deep into nature, and then you will understand everything better.. Albert Einstein. . one-gene, one-enzyme hypothesis. Many enzymes are composed of more than one polypeptide chain, or subunit (that is, they have a quaternary structure). In this case, each polypeptide chain is specified by its own separate gene. Thus, it is more correct to speak of a one-gene, one-polypeptide relationship: The function of a gene is to control the production of a single, specific polypeptide.. 69NB. Directions:. Using the DNA strand as a template, transcribe mRNA. Make sure to use the correct Nitrogen bases.. ATA CCT TAA CGC GTC. TAT TAG GCA AAA TTC. GTG TGA TTA ATA GCC. CTA AAG GAA TAG GAT. 4B investigate and explain. cellular processes, including homeostasis, energy conversions, transport of molecules, and synthesis of new molecules; 6C explain the purpose and process of transcription and translation using models of DNA and RNA; 9C identify and investigate the role of.