PPT-RNA Synthesis (Transcription)

Dr Shumaila Asim Lecture 2 1 RNA Synthesis Transcription Introduction First step in gene expression RNA is involved in the transfer of genetic information stored in DNA to proteins . and Its Regulation. January . 21 . –Mechanism of Transcription Initiation. January . 23– Regulation of of Transcription Initiation. January . 27–Mechanism and regulation of Transcription Elongation. Understanding:. Gene expression is regulated by proteins that bind to specific base sequences in DNA . The environment of a cell and of an organism has an impact on gene expression. Nucleosomes help to regulate transcription in eukaryotes. genotype to phenotype. Look deep into nature, and then you will understand everything better.. Albert Einstein. . one-gene, one-enzyme hypothesis. Many enzymes are composed of more than one polypeptide chain, or subunit (that is, they have a quaternary structure). In this case, each polypeptide chain is specified by its own separate gene. Thus, it is more correct to speak of a one-gene, one-polypeptide relationship: The function of a gene is to control the production of a single, specific polypeptide.. Chapters 16 and 17. Before the end of the semester we will be covering…. Historical DNA experiments. Structure of DNA/RNA. DNA Replication. Protein Synthesis (Transcription and Translation). Mutations. i. n Eukaryotes. Lecture #2. 1/23/17. References. . A few of the many insights from RNA polymerase structures. Cramer, P. (2002) . Multisubunit. RNA polymerases. . Curr. . Opin. . Struct. . Biol. Topic 2.6 & 7.2. Understandings:. Transcription is the synthesis of mRNA copied from the DNA base sequences by RNA polymerase. . Gene expression is regulated by proteins that bind to specific base sequences in DNA.. Eukaryotic Cells – cells with a nucleus and many organelles. Organisms with nucleated cells belong to the largest classification group, Domain . Eukaryota. Four Kingdoms of Organisms which are eukaryotic are animal, plants, . Structure of RNA. Major Classes of RNA. Transcription in Prokaryotes. Transcription in Eukaryotes. Post-transcriptional Processing of Eukaryotic mRNA. Transcriptional Regulation in Prokaryotes:. the Lac Operon as an example. synthesis). A. Prokaryotes: RNA polymerase, . Promoters – sigma factor. B. Eukaryotes: RNA polymerases, . Promoters – transcription factors. . 1 strand of . dsDNA. used as template to synthesize complimentary . Chapters 16 and 17. Before the end of the semester we will be covering…. Historical DNA experiments. Structure of DNA/RNA. DNA Replication. Protein Synthesis (Transcription and Translation). Mutations. Copyright 2016 by the Rector and Visitors of the University of VirginiaRNA nucleotides or building blocks are structurally very similar to DNA and go by similar names The only exception is that RNA ha Copyright 2016 by the Rector and Visitors of the University of VirginiaSTAT3STAT3STAT3STAT3RNAABNucleus of a cellBackgroundProtein products made from genes will have specific functions in the cell One Chargaff's rule. states DNA of any species contains equal amounts of guanine and cytosine and also equal amounts of adenine and thymine. . This pattern is found in both strands of the DNA. . They were discovered by Austrian chemist Erwin Chargaff. Professional medical transcription services provided by SpectraScribe offer a streamlined solution for researchers seeking to extract meaningful insights from various research methodologies.