PPT-Eukaryotic Transcription

Eukaryotic Cells cells with a nucleus and many organelles Organisms with nucleated cells belong to the largest classification group Domain Eukaryota Four Kingdoms of Organisms which are eukaryotic are animal plants . Tim . Johnstone. BIOL1220. Spring 2010. Transcription Factor. a protein that binds to specific DNA . sequences to modulate the transcription of DNA to mRNA. The transcription factor TATA binding protein (blue) bound to DNA (red). Image by David S. . Central Dogma of Biology. Information flows from . DNA .  RNA  Proteins. Think of it as exchanging money in a different country: proteins cannot be built directly from DNA. Three main processes involved:. TSW – Recognize that the presence of a nucleus determines whether a cell is prokaryotic or eukaryotic. 6.12B. Cells. There are two . basic types . of cells. Prokaryotes. or prokaryotic cells . Eukaryotes. 2.3.1, 2.3.2: Liver cell. 2.3.3: Electron micrograph of liver cell (rat). Glycogen. 2.3.4: Prokaryotic VS Eukaryotic cell. FEATURE. Prokaryotic cell. Eukaryotic cell. DNA. Naked DNA. DNA associated. with proteins. Understanding:. Gene expression is regulated by proteins that bind to specific base sequences in DNA . The environment of a cell and of an organism has an impact on gene expression. Nucleosomes help to regulate transcription in eukaryotes. Copyright © 2002 Pearson Education, Inc., publishing as Benjamin Cummings. Eukaryotic Cell. 3. Eu. = True. Karyon. = Nucleus. B- The Eukaryotic Cell. Animal Cell. Plant Cell. Compare Animal and Plant cell. BNFO 602/691. Biological Sequence Analysis. Mark Reimers, VIPBG. Why TFBS?. Protein transcription factors were among the first gene regulators identified. Many TFBS have distinct sequences, which were suitable for bioinformatics analysis. Structure. Central Dogma. Template Strand. Coding Strand. RNA Functions. RNA Building Blocks. Structure. Eukaryotic Process. RNA Polymerase. Subunits (Prokaryotes). α (2). β. β’. ω. σ. Transcription. Two Basic Types. Remember….cells are the basic unit of life for ALL living things.. There are two basic types of cells:. Prokaryotic cells . – found in bacteria. Eukaryotic cells . – found in . Eukaryotic Cell. 3. Eu. = True. Karyon. = Nucleus. B- The Eukaryotic Cell. Animal Cell. Plant Cell. Compare Animal and Plant cell. What are the functions of cell organelles . ?. Copyright © 2002 Pearson Education, Inc., publishing as Benjamin Cummings. Gene Expression. Gene Expression. Transcription. Splicing. Polyadenylation. mRNA Stability. Translation. Protein Stability. Controls on Protein Levels. Transcription Control - Prokaryotic Promoter. Transcription Control - Prokaryotic Promoter. Eukaryotic translation. Eukaryotic mRNAs are characterized by two post-transcriptional modifications: the 5-terminal 7methyl-GTP cap and the 3-terminal poly(A) tail .. The 7methyl-GTP cap is essential for mRNA binding by eukaryotic . Central Dogma. The direction of the . flow of genetic information. is from DNA to RNA to polypeptide.. DNA Replication. Initiation and Unwinding of DNA. DNA . gyrase. . (. topoisomerase. ) unwinds the DNA coil.. A eukaryotic transcription initiation complex consists of RNA polymerase II, . five. general transcription factors (GTFs), and a 20-subunit complex called Mediator (or . Srb. /Med). The CTD of RNA polymerase II anchors Mediator to the polymerase. Mediator allows RNA polymerase II to communicate with transcriptional .