PDF-Proc.Natl.Acad.Sci.USAVol.90,pp.5757-5761,June1993BiochemistryIncrease

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5758BiochemistryReisheretaloligodeoxynucleotide5CGTTCTCGACCGACGAAICTCTGACGCTCTTCCTCCAGGGGGTGCCGGGA3Thelast34ntunderlinedarecomplementarytothe5endofhumanapoAImRNASeveralcDNAprobeswereusedf

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Proc.Natl.Acad.Sci.USAVol.90,pp.5757-5761,June1993BiochemistryIncrease: Transcript

5758BiochemistryReisheretaloligodeoxynucleotide5CGTTCTCGACCGACGAAICTCTGACGCTCTTCCTCCAGGGGGTGCCGGGA3Thelast34ntunderlinedarecomplementarytothe5endofhumanapoAImRNASeveralcDNAprobeswereusedf. Proc.Natl.Acad.Sci.USA90(1993)8129Stimulusaz0C.)ww-JcnnNeuron'sResponseFourierFundamentalsAverage*Response=;;~~~~~~Noise=N-RiREALFIG.1.Calculationofaganglioncell'saverageresponseandresponsenoiseto10cy Proc.Natl.Acad.Sci.USA90(1993)10391days.Whenthecellswereforamicroinjectionexperiment,coverslipswerefirstmarkedwithadiamond-tippentofacilitatethelocalizationandvisualizationofmicroinjectedcells.After8- Proc.Natl.Acad.Sci.USA90(1993)RESULTSElectronMicroscopyofPNA-DNAComplex.Weuseda(dA)98-(dT)98targetcontainedwithinasupercoiledplasmid,pA98,linearizedwiththeCfrlOIrestrictionenzyme,andchallengedthiswith Proc.Natl.Acad.Sci.USA90(1993)11479knownconcentration.DNAwasstoredin1mMTris,pH7.8/0.1mMEDTA.Forinjections,DNAwasdilutedinsaline(0.9%NaCl).VaccineTrials.Vaccinetrialsinmicewereaccomplishedbytheadminist Proc.Natl.Acad.Sci.USA93(1996)9293exonjunctionsinthespy-1andspy-2mutants,genomicDNAwassubjectedtoPCRandtheproductsweresequenceddirectly.RESULTSANDDISCUSSIONIsolationofspy-4.Thespy-4mutantwasisolatedby Proc.Natl.Acad.Sci.USA90(1993)solvedinapyrogenicwaterandthesolutionwasfilteredthrougha0.22-,mcelluloseacetatefilter.Thelaminarinfiltratewaslyophilizedandresuspendedtoaconcentrationof1.47mg/ml.Lipopoly 868CellBiology:OsbornandWeberFIGS.1-7.(Legendappearsatbottomofthefollowingpage.)Proc.Nat.Acad.Sci.USA73(1976) Proc.Nat.Acad.Sci.USA73(1976)869FIG.8.Recoveryofcytoplasmicmicrotubulesin3T3cellsafterColc Proc.Natl.Acad.Sci.USA75(1978)RESULTSWhennormalhumanglobinDNAwasdigestedwithEcoRIandprobedwithamixtureofa-and(3-globincDNAs,fivemajorfragmentswereseen(Fig.1).Thelargestfragment,about21kilobases(kb)inl I,fromFlavobacteriumokeanokoites,isamemberoftheunusual,typeIIsclassofrestrictionendonucleasesthatrec-ognizeaspecificDNAsequenceandcleavenonspecificallyashortdistanceawayfromthatsequence(1).Ibindstheco Proc.Natl.Acad.Sci.USA90(1993)pHP45QC(13)bySmaIdigestionandgelpurification.pIB415isaderivativeofpIB410(ofIncPreplicon)containingvirAA63/240,avirAderivativelacking178aminoacidsintheperiplasmicdomain(14 TheNunproteinofbacteriophageHK022isamemberofthearginine-richmotiffamilyofRNAbindingproteins,whichincludesthephageNtranscriptionantiterminationproteinandtheHIVTatandRevproteins(Fig.1;refs.1±3).Nunbind Proc.Natl.Acad.Sci.USA88(1991)5593Table1.YeaststrainsNuclearMitochondrialStraingenotypegenotypeRef.947aade2ura3-52p09ID41-6aadellysi15161p+15C1036mit-;COXJintron116splicing-defectivepointmutant(trans- Cellsrespondtodevelopmentalcuesandenvironmentalstimulibyalteringgeneexpression.Often,transmissionofdifferentupstreamsignalsinvolvescommoncomponentsyetelicitsdistinct(butappropriate)outcomes.Numerousag Proc.Natl.Acad.Sci.USA92(1995)11999BacterialcellEukaryoticcellCalmodulinActive*-*tJ~'X-cyclaseATPcAMPFIG.1.Strategytoanalyzeinternalizationofbacterialproteinsinsidethecytosolofeukaryoticcells.sec,Gene

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