PPT-Determining the Histone Post-translational Modification Binding Specificities of ASH1L

Tiffanie Lee Quantitative Biology Dr Brian Strahl Department of Biochemistry and Biophysics How does ASH1L read chromatin What histone reader domains are involved in binding ASH1L and MLL cooperate to maintain hematopoiesis and transcription in immune cells in the blood. Y. Lyse & Sonicate. IP. Reverse crosslinks. Total. Reverse crosslinks. Amplify. Amplify. Sequence. Sequence. Immuno. -precipitation. Other controls for IP. (e.g., no antibody, non-specific antibody). Biological Sequence Analysis. BNFO 691/602 Spring . 2014. Mark Reimers. Analysis of ChIP-Seq Data. Genomic Data Analysis Course. Moscow July 2013. Mark Reimers, . Ph.D. What Are the Questions?. Where are histone modifications?. of epigenetics. A. dult. . stem cells know their . fate. !. For example: m. yoblasts. . can . form . muscle . cells. only.. Hematopoetic. . cells only become blood . cells. .. .....b. ut. . all . trithorax. -Group (. trxG. ). Maintain the “on” state of developmental regulatory genes. trx. : founding member (HMT on H3K4). . Transcriptional memory by . trxG. and . PcG. trxG. – on state memory. medicine. Part 2. Epigenetics. and Inheritance. Epigenetics. Epigenetics is generally defined “as relating to or arising from non-genetic influences on gene expression”.. Epi. is the Greek prefix meaning. Chromatin is made of repeating units of nucleosomes, which consist of 146 base pairs of DNA wrapped around an octamer of four core . histone proteins (H3, H4, H2A and H2B). Introduction. Histones are a special group of proteins found in the nuclei of eukaryotic cells responsible for DNA folding and chromatin formation.. 1. Bioinformatics III. X-ray structure of the nucleosome core particle consisting of core histones, and DNA. Top view.. www.wikipedia.org. Side view shows two windings of DNA and two histone layers. The DNA of eukaryotic organisms is packaged into chromatin, whose basic repeating unit is the . Nucleosome. A packaging unit for DNA (=H3/H4 tetramer + two sets of H2A/H2B . dimer. ). DNA (- charge) and . histones. (+ charge) . histones. = tails and globular domains. Higher-Order Packaging of Chromatins. 4. th. December 2013. 1. Understanding the Human Genome: Lessons from the ENCODE project. Austen Ganley. INMS. Glossary. Genome. Genes. DNA/RNA. Protein. Cell. Transcription. Chromatin. Histones. Nucleosomes. quantitation. . -. For most K residues our histone assay, we monitor the possible occurrence of difference modifications (. m. e1. , . m. e2. , . m. e3. ,. Ac. ) and . unmodified. peptide. . -Different forms of the same peptide, apart of their mass differences, can have different retention times (Important for . Thimo Rohlf 1,4 , Lydia Steiner 1,2 , Jens Przybilla 1 , Sonja Prohaska 2 , Hans Binder 1,3 and Jörg Galle 1 * 1 Interdisciplinary Centre for Bioinformatics of Leipzig University, D-04107 Leipzig, histones. (50%). Five major . histones. ; H2A, H2B, H3, H4 and H1 highly basic (. Arg. and Lys >25%). Roger Kornberg in 1974; . Chromatin. is made up of repeating units called . nucleosomes. , each containing 200 . Routes to effective glycopolymer inhibitors of bacterial toxins. s. -j.richards@warwick.ac.uk. . Sarah-Jane Richards. and Matthew I. Gibson. Department of Chemistry, University of Warwick, UK. @. LabGibson. 1. Bioinformatics III. X-ray structure of the nucleosome core particle consisting of core histones, and DNA. Top view.. www.wikipedia.org. Side view shows two windings of DNA and two histone layers. The DNA of eukaryotic organisms is packaged into chromatin, whose basic repeating unit is the .