Advancing the art of precast gel electrophoresis Criterion Precast Gels Simply what youd - PDF document

SimplywhatyouÕd expectfromBio-Rad:Pour in buffer Drop inMasterfulresultsClose the lid and The Standard in Precast Gel SystemsCriterion is a revolutionary miniprecast gel electrophoresis system that is remarkably easy to use and offers the ultimate in precast gels.system is the cassettesÕpatented* integrated buffer chamber, which simplifies assembly and ensures that theto slide the gels into place withoutEasily run a single gel or two gelswithout the need for buffer dams.Replaceable electrode carrierswith prestrung platinum wires evenmakeelectrode changes easy. Using the Cell Is Simple Drop in cassettes Pour in buffer and load samples Close the lid and start your run!* US patents #5,073,246 and #5,656,145 thattranscendthe The Criterion cell lid features a built-in wedgewedge means there is no extra tool to number, expirationbuffer type are printedfeatures five comb styles,including three multi-well formats(12+2,18-, and 26-well), two IPG combs for 2-Delectrophoresis, and a prep comb.the gel to be lifted easily from thecassette and to lie flat on filter paper,From patented sample loading guides that direct yourloading tip directly to the bottom of each well, to outlinedand numbered wells printed on each gel cassette, the 12 34 56789101112 131415161718An 18-well Criterion Tris-HCl 4Ð20% gradient gel is capable of running twice as many samples as a standard mini gel.Lanes 1 and 18, Precision Proteinelectrophoresis standards; lanes 2 and 10, chicken extract; lanes 3 and 11, shrimp extract; lanes 4 and 9, fish extract; lanes 5 and 13, ADH; lanes 6 and 16, catalase; lane 7, snake venom; lane 8, E.colilane 12, peroxidase; lane 14, raccoon serum; lane 15, urease; lane 17, myosin.Separate More Samples in Fewer RunsCriterion gels offer the sample capacity to outperform anyother precast gel system. Using12+2,18-,or 26-well combs,you can analyze more results on a single Criterion gel than ontwo standardmini gels Ñ and a Criterion Tris-HCl gel runs inas little as 45 minutes to further maximize your time savings.Resolve More Proteins on Every Gelprovide more 2-D spots on a single have a separate well for standardsand are designed to hold 11cm ReadyStrip IPG strips, providing35% more focusingarea in your first-dimension separation and 20% more gel area in the second dimension. Run 12 gels consistent, sharply resolved spots every time. dinary Mouse liver extract separated on an 11 cm ReadyStripIPG strip, pH 3Ð10, followed by second-dimension separation on a 4Ð12% Criterion XT Bis-Tris gelwith Precision Plus Proteinstandards. The gel was run with XT MOPS buffer and stained with SYPRO Ruby protein gel stain. 6.5%10–20% 5%10%15% 15%4–20% 5%7.5%10%12.5%15%18%4–15%4–20%8–16%10–20%10.5–14% 17.06.53.51.414.026.617.06.53.51.414.0 15001000700500400300200100501000200180160140120100806040201248064513230161418 15001000700500400XC300200100BPB50200015001000700500400300200XC10050200015001000700500400300200XC1005080XC60700500 11697.4 116664597.4 97.4 116664521.53197.4 14.4200116664521.597.4 14.4200116664521.597.46.5 14.4200116664521.597.46.5 14.4200116664521.597.46.5 14.4200116664521.597.46.5 14.4200116664521.597.46.5 14.4200116664521.597.4 ris-HClTris-TricineTBETBE-UreaBis-Tris and Tris-Acetate Gels For SDS-PAGE and Native PAGECriterion XT precast gels enable theseparation of proteins using just two gelbuffer systems: Bis-Tris gels for small tomid-size proteins and Tris-acetate gels forlarge proteins. Criterion XT gels aredelaying acrylamide hydrolysis compared Criterion XT Tris-acetate gels do not containSDS and can be used with Tris-glycinebuffer when native PAGE conditions areequired. Our nonreducing, nondenaturingXT sample buffer maintains proteinsecondary structure and native chargedensity for native PAGE conditions. ForSDS-PAGE applications, the sample buffershould be supplemented with XT reducingand SDS to ensure denaturation of thesample proteins.For SDS-PAGE and Native PAGESDS-PAGE is the most common choice forprotein electrophoresis. SDS-PAGEseparates complex protein mixtures bythe molecular weights of sample proteins.Bio-RadÕs Tris-HCl gels are made withoutSDS so that, by also omitting SDS from therunning buffer, they can be used to runproteins under nondenaturing conditions foractivity. Native analysis does not provideris-Tricine Gels is-Tricine gels are ideal for separation ofpeptides and small proteins with molecularweight esolution isachieve separation from the faster-movingSDS micelles that interfere with peptideesolution in Tris-glycine buffer systems.IEF separates proteins based on their netgels are cast with carrier ampholytes tocreate a pH gradient within the gel. Proteinsat which their net charge is zero. IEF gelscasein, which are substrates for variousproteases. Samples are run undernonreducing conditions, then allowed toenature and consume the substrate.Samples with proteolytic activity can bebackground after Coomassie Blue TBE gels are suitable for the electrophoresisof nucleic acids from 50 to 2,000 basepairs. TBE gels are ideal for analysis of thepurity of PCR products, standard dsDNAanalysis, and RNase protection assays.TBE-Urea Gels Denaturing TBE-urea gels arebetween 60 and 200 bases are resolved asprotection assays, and northern blotting. Bio-Rad Offers a Precast Gel for Virtually Every ApplicationMigration Charts of Protein Standards on Criterion Gels*(Sizes in kD except for TBE and TBE-urea gels, where sizes are in bp; IEF and zymogram patterns not included.) melessBeautyXTAdvantages12-month extended shelf life for Bis-Trisgels and 8 months for Tris-acetate gels Room temperature storage: no morefrigerator crowding problemsOptimized running buffers for equivalentmigration patterns to other neutral-pH gels Buffer sample sizes for low-cost trial runs addition to the Criterion productline, are the most convenient,highest-throughput, highest-quality extended-shelf-life precastprovide a shelf life of up to 12months at room temperatureand numerous benefits of theCriterion XT precast gels areformulated at a pH near neutrality,hydrolysis. Specially optimizedbuffers result in tighter, betteresolved bands throughout theentire life of the gel. to large proteins using just two gel buffer systems: Criterion XTBis-Tris precast gels for small tomid-sized proteins and Criterion XTris-acetate precast gels for large proteins. 1502501007550372520 15010075503725201510 150100755037252015 150100755037 150100755037 15010075503725201510 25010075503725201510 25010075503725201510 10%12%4–12% 10%12%4–12% 7%3–8% with XT MESrunning buffer: idealfor small proteinsBis-Tris gelswith XT MOPSrunning buffer: idealfor mid-size proteinsTris-acetate gelswith XT Tricinerunning buffer: idealfor large proteins If you use ÉThen go with É10Ð20%, 16.5% peptide/10%, 12%, 4Ð12% Bis-Tris gels with XT ricine gelsMES buffer 10% Tris-HCl gels10% Bis-Tris gels with XT MOPS buffer 12% Tris-HCl gels12% Bis-Tris gels with XT MOPS buffer 15%, 4Ð15%, 4Ð20% Tris-HCl gels 4Ð12% Bis-Tris gels with XT MOPS buffer 5% Tris-HCl gels3Ð8% Tris-acetate gels with XT Tricine buffer7.5% Tris-HCl gels7% Tris-acetate gels with XT Tricine buffer Criterion XT gels separate more samples in fewer runs. Precision Plus Proteinstandards separated on a 12% Criterion XT Bis-Tris 18-well gel with XT MOPSbuffer (left) and on a competitorÕs 12% Bis-Tris 10-well mini gel (right).ith a 12-month shelf life, new Criterion XT gels give you beautiful results anytime. Ordering InformationDescription12+2 Well Comb***18-Well Comb26-Well Comb*Prep+2 Well Comb** IPG+1 Well Comb**IPG-Well Comb45 µl Samples30 µl Samples15 µl Samples800 µl Samples11 cm IPG Strip11 cm IPG StripCriterion XT Bis-Tris Gels10% Resolving Gel345-0111345-0112345-0113345-0114345-0115345-011612% Resolving Gel345-0117345-0118345-0119345-0120345-0121345-01224Ð12% Resolving Gel345-0123345-0124345-0125345-0126345-0127345-0128Criterion XT Tris-Acetate Gels3Ð8% Resolving Gel345-0129345-0130345-0131345-0132345-0133345-01347% Resolving Gel345-0135345-0136345-0137345-0138345-0139345-0140Criterion XT Buffers and Reagents161-0788XTMOPS Running Buffer, 20x, 500 ml 161-0789XTMES Running Buffer, 20x, 500 ml161-0790XT Tricine Running Buffer, 20x, 500 ml161-0791XT Sample Buffer, 4x, 10 ml161-0792XT Reducing Agent, 1 ml161-0793XTMOPS Buffer Kit, includes 500 ml 20x XT MOPS runningbuffer, 10 ml 4x sample buffer, 1 ml XT reducing agent161-0796XT MES Buffer Kit, includes 500 ml 20x XT MES running buffer, 10 ml 4x sample buffer, 1 ml XT reducing agent161-0797XT Tricine Buffer Kit, includes 500 ml 20x XT Tricine runningbuffer, 10 ml 4x sample buffer, 1 ml XT reducing agent**Includes reference well(s). Gel dimensions (W x L)8.7 x 13.3 cm Gel thickness 1.0 mm Cassette dimensions 10.6 x 15 cm Cassette material Styrene copolymer Comb materialPolycarbonate Storage tray material PETGel storage conditions Room temperature for Bis-Tris gels 4¡C for Tris-Acetate gelsGel shelf life 12 months for Bis-Tris gels8 months for Tris-Acetate gelsake advantage of comprehensive, in-persondemos, easy-to-follow literature, and more. schedule a demo or for more information,visit us on the Web at or contact your local Bio-Rad representative. Criterion XT Specifications and Ordering InformationFor specifications and ordering information for other Criterion precast gels, see back page. Life Science Bulletin 2710 US/EG Rev C (86-21) 63052255 Gel dimensions (W x L)8.7 x 13.3 cmGel thickness 1.0 mmCassette dimensions 10.6 x 15.0 cmCassette material Styrene copolymerComb materialPolycarbonateStorage tray material PETGel storage conditions Store flat at 4¡C; do not freezeGel shelf life ~12 weeks for Tris-HCl, Tris-Tricine, TBE, zymogram, TBE-urea; ~26 weeks for IEFOrdering Information+2 Comb*18-Well Comb26-Well Comb*Prep+2 CombIPG Comb45 µl Samples30 µl Samples15 µl Samples800 µl Samples11cm IPG StripCriterion Tris-HCl Gels (All Tris-HCI gels except 4Ð15% and 4Ð20% gels have a 4% stacking gel.)5% Resolving Gel 345-0001345-0002345-0003345-0004ÑÑ.5% Resolving Gel345-0005345-0006345-0007345-0008ÑÑ0% Resolving Gel345-0009345-0010345-0011345-00122.5% Resolving Gel345-0014345-0015345-0016345-00175% Resolving Gel 345-0019345-0020345-0021345-0022ÑÑ8% Resolving Gel345-0023345-0024345-0025345-0026ÑÑ5% Gradient Gel345-0027345-0028345-0029345-003020% Gradient Gel345-0032345-0033345-0034345-00356% Gradient Gel345-0037345-0038345-0039345-004020% Gradient Gel345-0042345-0043345-0044345-004510.5Ð14% Gradient Gel345-9949345-9950345-9951345-9952IEF Gel, pH 3Ð10345-0071345-0072345-0073345-0074ÑÑIEF Gel, pH 5Ð8 345-0075345-0076345-0077345-0078ÑÑ0% Gel, gelatin345-0079345-0080345-0081ÑÑÑ2.5% Gel, casein345-0082345-0083345-0084ÑÑÑCriterion Tris-Tricine/Peptide Gels6.5% Gel345-0063345-0064345-0065345-0066ÑÑ20% Gradient Gel345-0067345-0068345-0069345-0070ÑÑ5% Gel345-0047345-0048345-0049345-0050ÑÑ0% Gel345-0051345-0052345-0053345-0054ÑÑ5% Gel345-0055345-0056345-0057345-0058ÑÑ20% Gradient Gel345-0059345-0060345-0061345-0062ÑÑCriterion TBE-Urea Gels5% Gel345-0085345-0086345-0087ÑÑÑ0% Gel345-0088345-0089345-0090ÑÑÑ5% Gel345-0091345-0092345-0093ÑÑÑCatalog #DescriptionCriterion Mini Vertical Cells and Blotters165-6001Criterion Cell, includes tank, lid with power cables, 3 sample loading guides to match12+2,18-well,and26-well combs,ins165-4130Criterion Dodeca Cell, includes tank and lid with power cables, instructions170-4070Criterion Blotter With Plate Electrodes, includes tank, lid with cables, 2 gel holder cassettes, filter paper (50 sheets), 4 fiber pads, gel blot assembly tray and roller, sealed ice cooling unit, instructions170-4071Criterion Blotter With Wire Electrodes, includes tank, lid with cables, 2 gel holder cassettes, filter paper (50 sheets), 4 fiber pads, gel blot assembly tray and roller, sealed ice cooling unit, instructions65-6006Criterion Sample Loading Guide,12+2 well,165-6007Criterion Sample Loading Guide, 18-well,165-6008Criterion Sample Loading Guide, 26-well,1Criterion Membrane/Filter Paper Sandwiches 162-02320.2 µm Nitrocellulose/Filter Paper Sandwich, 20 pack162-02330.2 µm Nitrocellulose/Filter Paper Sandwich, 50 pack162-02340.45 µm Nitrocellulose/Filter Paper Sandwich, 20 pack162-02350.45 µm Nitrocellulose/Filter Paper Sandwich, 50 pack162-0236Sequi-Blot162-0237Sequi-Blot PVDF/Filter Paper Sandwich, 50 packCoomassie is a trademark of Imperial Chemical Industries PLC. SYPRO is a trademark of Molecular Probes, Inc. with integral buffer chamber,0.5 x15.0 cm, runs exclusivelygel is 13.3 wide x8.5 cm longand 1.0 mm thick. Criterion gels. Lower bufferbuffer chamber/Runs with 6 L of buffer.efficient, transferring most proteins in 30 min to 1 hr. 1.3 L of buffer. Advancing the art of precast gel electrophoresis CriterionPrecast Gels