Isotopeswhich have same chemical but different physical properties having radioactivity Natural occurrence is rare because of its instability They emit different radiations such as α β ID: 774782
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Slide1
AUTORADIOGRAPHY
Slide2Radioisotopes
Isotopes(which have same chemical but different physical properties) having radioactivity.
Natural occurrence is rare because of its instability.
They emit different radiations such as
α
,
β
and
γ
.
Slide3Detection of disintegration:
Electrical
Eg
: GM counter,
Ionisation
counter, Gas flow counter
Scintillation
Eg
: scintillation counter
Autoradiography
Slide4What is autoradiography…?
Slide5Principle
Incubate tissue with
radioactive
ligand
Expose to film
or emulsion
Isotope will emit
radiation (usually beta)
Radiation will hit silver grains in emulsion and expose them
Slide61867 Uranium salts responsible for blackening of photographic film(Niepce de st.victor) 1896 K2UO2(SO4)2emits radiation affecting a photographic film(Becquerel)1924 First autoradiogram –distribution of Po in biological material1943 Autoradiographic demonstration of 131I in tissue sections of thyroid 1946 Liquid photographic emulsion for autoradiography(Belanger and LeBlond)1950 ’Stripping film’ technique for microscopic autoradiography1970 …Numerous biological applications… ~1990 Alternatives to film: Imaging plate technology
HISTORY
Slide7PROCEDURE
Slide8Types:
In-vivo autoradiography
In-vitro autoradiography
Slide9Cont…
MICROSCOPIC
AUTORADIOGRAPHY
Hypophysis
of rat
labelled
with
3
H
Slide10Macroscopic autoradiography
Whale body autoradiography
Localization on chromatogram or gel
Slide11Interesting, right?
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