PDF-Fluorescence and Scanning Electron Microscopy of ChitosanDNA Nanoparticles for Biological

Masotti F Marino G Ortagg i and Cleofe Palocci Department of Chemistry University of Rome La Sapienza Pza Aldo Moro 5 00185 Rome Italy Polysaccharides and other cationic polymers have been recently used in pharmaceutical research and industry for t. Lecture 13:. Super-resolution microscopy: Part I. Lecture 13: . Fluorescent labeling, multi-. sprectral. imaging and FRET. Review of previous lecture. FRET. FLIM. Super resolution . microscopy. NSOM. II. MENA3100,OBK, . 29.01.15. We don’t read all. You don’ have to read all. 1.3 Specimen Preparation. Read it. 1.4.1 Bright-Field and Dark-Field. Definetly. 1.4.2 Phase-Contrast. Cursori. 1.4.3 Polarized-Light. Jenny . Malmstrom. AFM invented by Binning and co-workers in 1986. . Belongs to the Scanning Probe Microscopy family. Binning et al., Physics Review Letters 1986. AFM invented by Binning and co-workers in 1986. . Peter Harris. Transmission . electron . microscopy. ► What . is transmission electron microscopy?. . ► TEM in biological science . ► CfAM’s TEMs. ► TEM in physical . science . ► Recent developments in TEM. Outline. Introduction. Optical Microscopy. Types of Optical Microscopes. Confocal Microscopy. Laser Scanning Confocal Microscopy. Examples. Optical Microscopy. Optical microscopy is the oldest type of microscope and uses visible light and a system of lenses to create an image of the sample. . Electron microscopy (EM) . is an electron beam which is focused into a small probe across the surface of a specimen . . The first electromagnetic lens was developed in 1926 by . Hans Busch. .. . are held together at their centromeres binds specific proteins, which in turn make up a disk-like structure called the kinetochore. The kinetochore is an attachment site for spindle fibers, which pla (SEM) Electron Microscopy (SEM) and TEM Scanning electron microscopy is used for inspecting topographies of specimens at very high magnifications using a piece of equipment called the scanning electr COLLEGE BOTANY: SEM - VI, PAPER - DSE4T: ANALYTICAL TECHNIQUES IN PLANT SCIENCES, UNIT - 1: FLUORESCENCE MICROSCOPY What is fluorescence microscopy? A fluorescence microscope is an optical microscope Ng M, Lee J, Leong M, Ling A, Tan H, Ooi E. Topographic Changes in SARS Coronavirus–infected Cells during Late Stages of Infection. Emerg Infect Dis. 2004;10(11):1907-1914. https://doi.org/10.3201/eid1011.040195. A . fluorescence microscope.  is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to . study . organic . or inorganic substances. and. Other Characterization Methods. Schedule for next 3 weeks. Week 9: Oct. 18 – Ch. 7 quiz, SEM/Characterization. . Oct. 20 – No class, SEM virtual reality lab. Week 10: Oct. 25 – Fabrication methods for nanotechnology. The aims of this course are:. ● . to introduce the principles of scanning electron microscopy. ● . to describe the components of the microscope and explain how they work. ● . to highlight some of the problems which can arise during imaging. ELECTRON. . MICROSC. O. PY. . . . Electron. . micros. c. opy. . (EM). . is a technique. . u. s. ed. . for. . obtaining. . high re. s. olu. t. ion. . images. . of. . biological and non-biologi.