Virtual Lab Seed Experiment - PowerPoint Presentation

Slide1

Virtual Lab Seed Experiment

AND Setting up the Research Biology Notebook (a tutorial for you!)

DAY 2:

Examining Seeds Slide2

Virtual Seed Lab – Day 2

Objective:

Students will:

Know the parts of the seed;

Understand requirements for seeds to germinate and grow;

Be able to explain and analyze the growth patterns of seedlings.

Recognize differences between plants in their germination and growth requirementsSlide3

Virtual Seed Lab – Part 2

In Part 1 you examined seeds and discovered what seeds need to germinate and grow. We will use this information to examine the following research

question:

How

do seedlings develop over time?

Think about how we can obtain

evidence

to answer this question.

(

Hint: scientific evidence is obtained by

observations and measurements

to prove a hypothesis or theory)

How can we obtain

observations and measurements

to help answer the research questions?

We can perform and experiment!Slide4

Virtual Seed Lab

Remember that the scientific method is an ongoing process http://undsci.berkeley.edu/article/scienceflowchart

 Slide5

Virtual seed lab

Parts of an Experiment –

to be determined in every experimentSlide6

Virtual Seed lab:

How do seedling develop over time?What is the IV for the Virtual Seed Lab?

time

What is the DV?

Measurement of root and shoot lengthSlide7

Research Lab Notebook

- lets get started:Page 1: Title Page

Page 2 and 3: Table of Contents

Page 4 leave blank (just in case)

Page 5 and 10: Label with:

Page number

Title of Lab/experiment OR Research Question?

Did you remember to add the date, title and page number to your Table of Contents?Slide8

Set up your Title Page:

(left

side)

(leave blank)

.

(right

side)

Example:

Research Biology

2015-2016

Jane/john

Doe

Riverside High School,

Leesburg, VA 20157

1.Slide9

Does your table of contents look like this?

(left

side)

(Leave blank)

.

(right

side)

Table

of Contents

Date Title Page Number

9/9/16 Virtual

Seed Lab

2.

Set up 2 page (2 and 3) for the title pages)Slide10

Does your table of contents look like this?

(left

side)

(Leave blank)

.

(right

side)

Table

of Contents

Date Title Page Number

3.

Set up 2 page (2 and 3) for the title pages)Slide11

Does your table of contents look like this?

(left

side)

(Leave blank)

.

(right

side)

(leave blank)

4.Slide12

Lets add our Model to the

(left side)

(right side)

Date VIRTUAL LAB SEED EXPERIMENT

5.

Research Model

:

Research Question

:Slide13

Virtual Lab Seed Experiment

In your notebook under Student Understandings:

Write down 4 observations after examining the seeds (pictures on next slide)

Draw a germinating seed and label the parts

Write down two questions of your own about the experiment?Slide14
Slide15

Germinating seed

(root)

(Shoot)Slide16

Add more pages to this experiment:

(left side)

(right side)

Date VIRTUAL LAB SEED EXPERIMENT

6.

STUDENT UNDERSTANDINGS:

Write

1-2 sentences about

why you think the lab is

important to our

understanding of science.

References:

Https//

askabiologist.asu.edu

/experiments/

vpocketseeds

:

Slide17

Lets get going with the content:

(left side)

(right side)

Date VIRTUAL LAB SEED EXPERIMENT

EXPERIMENTAL DESIGN:

7.

Independent Variable:

Experimental Groups

Dependent Variable:

Control:

Constant:

we will add later

Research Question:

Hypothesis:

Null (Ho) Hypothesis

A

lternative (Ha) hypothesis

we will add laterSlide18

Virtual Lab Seed Experiment

Part 2:Examine the contents of the germinated seed packetsIn your notebook, under materials, write down the materials needed for this labSlide19
Slide20

Lets get going with the content:

(left side)

.

(right side)

Date VIRTUAL LAB SEED EXPERIMENT

8.

MATERIALS: (listed with bullets)

METHODS/PROCEDURES: (numbered steps)

MATERIALS: (listed with bullets)Slide21

List of Materials from virtual lab:

Seed

packets (this experiment uses Peas, Little Marvel)

Re-sealable

plastic bags (sandwich or quart size)

Paper

towels or napkins

Water.

Tape

Cardboard

(optional)

Paper

clips (optional)

Binder

clips (optional)

ScissorsSlide22

Virtual Lab Seed Experiment

Part 2 (all right side):

We are going to observe the effect of the environment on seed germination and growth?

Go to

https://askabiologist.asu.edu/experiments/pocketseed_compare#plant_results

With your group decide on a Research Questions and pick 1 conditions that you would like to compare (your control is chosen for you and the IV is your selection)

In your notebook on under

Experimental Design write down the

:

Research Question (put under title)

The control – Seeds Soaked overnight (10

hrs

light and 14

hrs

Dark at 23

o

C

IV- (what are you manipulating and/or comparing to the control?) Choose

ONE

of the following:

Soaked and rotatedNot soakedSoaked and no lightDV – measurement of root and shoot growth (cm) = the responding variableSlide23

Part 2 – Your experiment

Since we do not have time to run this experiment from the beginning, you will be given seeds that have been germinated for 10 days by Dr Biology’s graduate assistant. Bethany Lane has collected so much information from the experiment on seed germination that he needs someone to analyze the data. All the results have been recorded in photographs, including some cool animations. However, Bethany is out sick today and you need to collect the

data from the other experimental group and compare it to the control data.

Your job is to examine the contents of the Pocket Seed Experiment for seeds that have been soaked overnight and placed in the light for 10 hours and in the dark for 14 hours and kept at 23

O

C. We will use this data as the control data for the experiment.

(this data has been done for you and is located on the Excel spreadsheet from Day 1 of this lab)Slide24

Part 3 – your experiment

Time for you to develop an experiment and answer your own research question:

After observing Dr. Biology’s data and analyzing the control seeds, it is time for you to design your own experiment. Observe the effect of the environment on seed germination and growth that has been collected from the experiment done by Dr. Biology.

Pick 1 condition (independent variable) that you would like to compare with the control from Dr Biology’s data Choose

ONE

of the following:

Soaked and rotated

Not soaked

Soaked and no light

Develop a research question to observe the effect of the environment on seed germination.

Go to Dr. Biology’s data site

https://askabiologist.asu.edu/experiments/pocketseed_compare

Compare seeds for days 1-10 for your environmental

condition

and fill in the data

on the excel spreadsheet. (under the

Seed Lab Blank Data Sheet)

Compare the data for your environmental condition to the control data by analyzing the graph for shoot and root length(previously completed in Part 2. Slide25

Add data for your variable?

Follow these directions:

To

compare the results from days

1-10

for the

variable that you picked ,

check the boxes for the days

1-10

for the seeds in

the row for your variable.

The boxes will turn blue once checked.

(important : you must also check the boxes for days 1-10 for the control plants. This data has been done for you but the program must compare both variables)

Once all 2

0

boxes are checked, click the “compare Plants” button at the bottom of the table. The comparison photos will open in a new window.

Click on

Day

1 card

of your variable to see the larger image (you will have the data for the control (A1) cards, but you do not need to take measurements for these seeds)Measure the root and shoot growth for each of the 3 seeds:The data for A1, Day 1 has been done for you but you can see that it is 0 since root and shoot have not begun to grow.Next click on the root for A1, Day 2 to add a point.Continue to add points for the entire length of the root. The total distance (cm) for the combined points will be displayed at the bottom of the card. (Hint: you will need to click on the root, take the measurement, delete the points and then click on the shoot and take measurements. Look to the upper right corner for instructions on how to delete points. The total distance shown at the bottom left corner is the combined points from the ALL the points that you selected to measure)Now take the measurement for the shoot for A1, Day 2 (it will be 0 also) Record the results in the data table using the excel spreadsheet for this lab. Repeat steps 2-6 for each day: A1 Day 2; A1 Day 3 etc. As the data table is filled in, the Average root length and the average shoot length is filled in the excel spreadsheet. Slide26

Virtual Lab Seed Experiment

collecting data:Compare the results from all 10 different days. Check the boxes for the days you want to see then click “Compare Plants”

Select the pictures to see larger images

Measure the length of the root and the shoot using the virtual measuring tool

Record measurements in

the data table in your

notebookSlide27

Virtual Lab Seed Experiment

Paste the table that you have been given in your lab notebook on Page 7 to record your data

                Slide28

Add your data

Add commentsThe graph is made for you.Be sure to save the file, print it

and paste it in your

lab notebook under

results section.

Slide29

Now lets set up the experiment pages?

(left side)

(leave blank)

(right side)

VIRTUAL LAB SEED EXPERIMENT DATE

9.

Data Tables

: (be sure to include a

Title for the table)Slide30

Analyzing the data:

The best way to see the results of an experiment is to make a graph. There is a graph at the bottom of the page that can be used to analyze the data on the excel spreadsheet. Copy the graphs for the Root and Shoot length for the CONTROL and your VARIABLE in your notebook.

Why did the experiment use a line graph to analyze and visualize the data?Slide31

Now lets set up the experiment pages?

(left side)

(leave blank)

(right side)

VIRTUAL LAB SEED EXPERIMENT DATE

10.

Graphs

:

Be sure to include the following for your

Graph:

Title (The Effect of the IV on the DV)

Dependent variable on Y axis

Independent variable on X axis

Axis labeled

Scales drawn on axis

Key included

Drawn neatly (with a ruler – no free

Hand drawing of your graph)Slide32

Point Graph

You could graph all the numbers on a point graph ( figure 1).The point graph shows more of the story, the difference looks bigger, and you can see that there’s one point that looks out of place (dotted circle). This point represents the one soaked seed that failed to grow. When this seed is included in the average for the bar graph, it lowers the average.

You might say that because one of the soaked seeds failed that means soaking causes seeds to fail more than not soaking. However, you cannot make that conclusion without first repeating the experiment to see if you get the same results. In fact, scientists often repeat experiments many times before they make any conclusions. Slide33

Bar Graph

You could graph the average of each type of measurement on a bar graph ( figure 2). The bar graph tells you that on day 10, there’s little if any difference between

unsoaked

seeds and soaked seeds Slide34

Line Graphs

Another way to answer the question is to measure the seed sprouts each day, and graph the averages on a line graph ( figure 3). When we look at the line graph, we see more of the story. It shows that

unsoaked

seeds take longer to sprout, and that on days 2-9 there is a bigger difference between the length of the seed sprouts, than there is on day 10, when the average seed sprout length is very similar.

As you can see from these graphs, you need to use the right graph and analysis tools to write and publish sound scientific results. Slide35

Compare these graphs:

Which one best represents the research question?Slide36

Results section – DATA

(left side)

(leave blank)

(right side)

VIRTUAL LAB SEED EXPERIMENT DATE

11.

Results

:

Be sure to include the following for your

Results:

Topic sentence (what is the lab about)

Identify the IV and the DV

State if the data is quantitative or qualitative

Include the means (AVG) for each group

What results were discovered?

State the null hypothesis.

Was the null hypothesis accepted or rejected?Slide37

Analysis of Data

Making Conclusions from the Data for the Control seeds on days 1-10:Look at the graph that was created on the excel spreadsheet. Do you notice any trends in the data?

Which days do the seeds start to grow a root, or a shoot? Do roots and shoots grow at the same rate?

Does the data help us to answer our research questions: How do seedlings develop over time?Slide38

Results section – DATA

(left side)

(leave blank)

(right side)

VIRTUAL LAB SEED EXPERIMENT DATE

11.

Conclusion

:

Be sure to include the following for your

Conclusion:

Topic sentence (what is the lab about)

What results were discovered?

State the null hypothesis.

Was the null hypothesis accepted or rejected?

What is the final conclusion – make sure

to state the conclusion and add a plausible

Explanation (this is the WHY)

6. Were there any sources of error?

7. What is the next step in this research or

Project?Slide39

Your Assignment

To be turned into your teacher on TurnItIn.com:

Answer the following questions:

What is your the Research Question?

What is the Independent Variable (environmental condition you choose)

What data did you choose to compare and why? (Day and condition)

Why does it answer the research questions?

Provide a summary of the

data from your tables and graphs – 1-2 sentences

Write

1-2 sentences describing your conclusions from this experiment

Write a sentence about the possible sources of error and next step (or question) for this experiment.

Be sure to save the file as:

LASTNAME_VIRTUAL_SEEDLABRESULTSSlide40

Submit to TurnItIn.com

For the first time:Research Biology block A1ID# 13378385Password:

ResearchBio

Research Biology block

A4

ID#

13268563

Password:

ResearchBioSlide41

All Done!!!

*** be sure to go back and fill in the information on

your Table of Contents!!!