PPT-  Bacteriophage

as a novel approach to Biofilm inhibition and remove Azizian Reza1 Azizi Jalilian Farid2 Askari Hasan1 Naser Ahmad1 Karimi Sajedeh1 Sadeghifard Norkhoda1 . Yvonne. . Lin. . AP. . Bio. . 2. a.k.a. Phages. Bacterial . VIRUS. Attacks, infects, and replicates within bacteria. Discovered. . by. . Frederick. . Twort. . (Great. . Britain, 1915). Species specific – named after the group or species it infects. Very complex shape, requiring 20 gene products for assembly. - . Capsid. (head), contains linear dsDNA genome. - . Tail. , consists of sheath plus internal tube. Bacteriophage T4. Figure 11.2. Phage T4 binds to . Nicholas . Celms. San Diego State University. Funded in part by NSF 0827278 UBM Interdisciplinary Training in Biology and Mathematics.. 1. Goal. Build a method for making taxonomic groupings of . bacteriophages. Kaleigh. , . mariam. , Michael and Nicholas . Start Associated Sequences in . Enterobacteriophage. Kaleigh. hedges . Ribosomal Binding Site . (Shine-. Dalgarno. ). http://. themedicalbiochemistrypage.org. Yang Xin. Paper presentation. PNAS 2009, 106(12):4629-4634. Introduction. Antimicrobial drug development is increasingly lagging behind the evolution of antibiotic resistance. as a result, there is a pressing need for new antibacterial therapies that can be readily designed. Welkin Pope. SEA-PHAGES Bioinformatics Workshop, . 2016. Virion structural and assembly genes. , i.e. those encoding proteins that are either components of virion particles or assist in their formation. These include genes encoding the . Mary Owen, Andrew . Schaff. , Kayla Cartwright, Angel Chen, Jessica Clark, Bradley Davis, Paige . Etchison. , Shelby . Frieszell. , Jacquelyn Gross, Steph Smith, Elsa Clenny, . Sammi. . Podolyan. , Stephanie . Bethany Yachuw. Phages. Phage life cycles. Transcription determines life cycle. RNA Polymerase. Repressor. Antirepressor. Find the putative antirepressor. Yuan et al (2014. ). Via conceptual translation . apsid. N. ucleic Acid. R. eceptors. DNA or RNA. Membrane around virus. Proteins that help virus get into proper host. Attachment. The virus attaches to receptors on the host cell.. The virus is able to get into the cell in one of three ways.. Unequivoca. l Proof . 1952. Worked with Bacteriophage. Attaches to bacteria Genetic Material in cell Cell treats it as its own More Virus particles synthesized. Whether protein has entered or DNA. Mary Owen, Andrew . Schaff. , Kayla Cartwright, Angel Chen, Jessica Clark, Bradley Davis, Paige . Etchison. , Shelby . Frieszell. , Jacquelyn Gross, Steph Smith, Elsa Clenny, . Sammi. . Podolyan. , Stephanie . A vector is a DNA molecule that has the abil­ity to replicate autonomously in an appropri­ate host cell and into which the gene of inter­est (a foreign genetic sequence) is integrated. When we insert a foreign genetic sequence into the vector the aim is either to obtain numer­ous copies of the gene of interest or to obtain the product of that.. A . virus. (from the Latin virus meaning toxin or poison) is a microscopic organism consisting of genetic material (. RNA or DNA. ) surrounded by a protein, lipid, or glycoprotein coat.. Structure of a virus. and Prokaryotic Populations in an Urban Coastal . Wetland, the . Ballona. Wetlands, . by . Confocal. . Microscopy. . Methods. Collection. 1 sample collection from the . Ballona. Wetlands during the ebb flow .