Outline I Photosynthesis A Introduction B Reactions II Cellular Respiration A Introduction B Reactions What organisms go through photosynthesis Producersautotrophs such as plants trees algae some bacteria ID: 670367
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Slide1
Photosynthesis and Cellular RespirationSlide2
Outline
I. Photosynthesis
A. Introduction
B. Reactions
II. Cellular Respiration
A. Introduction
B. ReactionsSlide3
What organisms go through photosynthesis?
Producers/autotrophs
,
such as plants, trees, algae, some bacteriaSlide4
Photosynthesis
Method of converting sun energy into chemical energy usable by cells
Autotrophs
: self feeders, organisms capable of making their own food
Photoautotrophs: use sun energy e.g. plants photosynthesis-makes organic compounds (glucose) from lightChemoautotrophs
: use chemical energy e.g. bacteria that use sulfide or methane chemosynthesis-makes organic compounds from chemical energy contained in sulfide or methaneSlide5
Factors that affect photosynthesis
1.
Water
– needed to start the LD reaction
2.
Temperature – proteins work best between 0-35 degrees Celcius3. Light – need light to excite e- in chlorophyllSlide6
Where does photosynthesis take place?
In the chloroplasts of plant cells found in the leaves!
Plant
Plant Cell
ChloroplastSlide7
Leaf Structure
Most photosynthesis occurs in the
palisade
layer.
Gas exchange of CO
2 and O2 occurs at openings called stomata surrounded by guard cells on the lower leaf surface.
Palisade
SpongySlide8
Chloroplasts have chlorophyll
Chlorophyll is a green pigment that reacts to sunlight by transferring energy to e- (electrons)
Makes chloroplasts and plants look green
Reflect green light waves from the sunSlide9
Pigments
Chlorophyll A is the most important photosynthetic pigment.
Other pigments called antenna or accessory pigments are also present in the leaf.
Chlorophyll B
Carotenoids (orange / red)
Xanthophylls (yellow / brown)These pigments are embedded in the membranes of the chloroplast in groups called photosystems.Slide10
Photosynthesis
Photosynthesis
takes place in specialized structures inside plant cells called
chloroplasts
Light absorbing pigment molecules e.g. chlorophyllSlide11
Parts of a Chloroplast
Granum
Thylakoid
(single sac)
Stroma (fluid)
Inner Membrane
Outer MembraneSlide12
Chloroplast Structure
Inner membrane called the
thylakoid
membrane.
Thickened regions called
thylakoids. A stack of thylakoids is called a granum. (Plural – grana)
Stroma
is a liquid surrounding the thylakoids.Slide13
Photosynthesis Chemical
Eqn
:
Sunlight + 6CO
2
+ 6H
2
O = C
6
H
12
O
6
+ 6O
2
carbon dioxide
water
sugar (glucose)
oxygen
Reactants
“what is used”
Products
“what is made”Slide14
Overall Reaction
6CO
2
+ 12 H
2O + light energy → C
6H12O6 + 6O2+ 6H2O
Carbohydrate made is glucose
Water appears on both sides because 12 H
2
O molecules are required and 6 new H
2
O molecules are madeWater is split as a source of electrons from hydrogen atoms releasing O2
as a byproductElectrons increase potential energy when moved from water to sugar therefore energy is required Slide15
Light-dependent Reactions
Overview
: light energy is absorbed by chlorophyll molecules-this light energy excites electrons and boosts them to higher energy levels. They are trapped by electron acceptor molecules that are poised at the start of a neighboring transport system. The electrons “fall” to a lower energy state, releasing energy that is harnessed to make ATP Slide16Slide17
Energy Shuttling
Recall
ATP
: cellular energy-nucleotide based molecule with 3 phosphate groups bonded to it, when removing the third phosphate group, lots of energy liberated=
superb molecule for shuttling energy around within cells.Other energy shuttles-coenzymes (nucleotide based molecules): move electrons and protons around within the cellNADP+, NADPH NAD+, NADP FAD, FADH
2Slide18
Light-dependent Reactions
Photosystem
: light capturing unit, contains chlorophyll, the light capturing pigment
Electron transport system
: sequence of electron carrier molecules that shuttle electrons, energy released to make ATPElectrons in chlorophyll must be replaced so that cycle may continue-these electrons come from water molecules, Oxygen is liberated from the light reactionsLight reactions yield ATP and NADPH used to fuel the reactions of the Calvin cycle (light independent or dark reactions)Slide19Slide20Slide21
Calvin Cycle (light independent or “dark” reactions)
ATP and NADPH generated in light reactions used to fuel the reactions which take CO
2
and break it apart, then reassemble the carbons into glucose.
Called carbon fixation: taking carbon from an inorganic molecule (atmospheric CO2) and making an organic molecule out of it (glucose)Simplified version of how carbon and energy enter the food chainSlide22Slide23
Photosynthesis
Light-Dependent Reaction
Light-Independent Reaction AKA Calvin Cycle
- Occurs in thylakoid membrane
- sunlight is required
O
2
is produced from water
e- fuel many reactions by going through Electron Transport Chain
- Occurs in stroma
sunlight is NOT required
Glucose is produced from CO
2
Slide24
Harvesting Chemical Energy
So we see how energy enters food chains (via autotrophs) we can look at how organisms use that energy to fuel their bodies.
Plants and animals both use products of photosynthesis (glucose) for metabolic fuel
Heterotrophs
: must take in energy from outside sources, cannot make their own e.g. animalsWhen we take in glucose (or other carbs), proteins, and fats-
these foods don’t come to us the way our cells can use themSlide25
Cellular Respiration Overview
Transformation of chemical energy in food into chemical energy cells can use: ATP
These reactions proceed the same way in plants and animals. Process is called
cellular respiration
Overall Reaction:C6H12O6
+ 6O2 → 6CO2 + 6H2OSlide26
Anatomy of MitochondriaSlide27
Cellular Respiration Overview
Breakdown of glucose begins in the cytoplasm: the liquid matrix inside the cell
At this point life diverges into two forms and two pathways
Anaerobic cellular respiration (aka fermentation)
Aerobic cellular respirationSlide28
C.R. Reactions
Glycolysis
Series of reactions which break the
6-carbon glucose
molecule down into two 3-carbon molecules called pyruvateProcess is an ancient one-all organisms from simple bacteria to humans perform it the same wayYields 2 ATP molecules for every one glucose molecule broken down
Yields 2 NADH per glucose moleculeSlide29Slide30
Anaerobic Cellular Respiration
Some organisms thrive in environments with little or no oxygen
Marshes, bogs, gut of animals, sewage treatment ponds
No oxygen used= ‘an’aerobic
Results in no more ATP, final steps in these pathways serve ONLY to regenerate NAD+ so it can return to pick up more electrons and hydrogens in glycolysis.End products such as ethanol and CO2
(single cell fungi (yeast) in beer/bread) or lactic acid (muscle cells)Slide31Slide32
Aerobic Cellular Respiration
Oxygen required=aerobic
2 more sets of reactions which occur in a specialized structure within the cell called the
mitochondria
1. Kreb’s Cycle2. Electron Transport ChainSlide33
Kreb’s Cycle
Completes the breakdown of glucose
Takes the pyruvate (3-carbons) and breaks it down, the carbon and oxygen atoms end up in CO
2
and H2OHydrogens and electrons are stripped and loaded onto NAD+ and FAD to produce NADH and FADH2Production of only 2 more ATP
but loads up the coenzymes with H+ and electrons which move to the 3rd stageSlide34Slide35
Electron Transport Chain
Electron carriers loaded with electrons and protons from the Kreb’s cycle move to this chain-like a series of steps (staircase).
As electrons drop down stairs, energy released to
form a total of 32 ATP
Oxygen waits at bottom of staircase, picks up electrons and protons and in doing so becomes water Slide36Slide37
Energy Tally
38
ATP for aerobic vs. 2 ATP for anaerobic
Glycolysis 2 ATP
Kreb’s 2 ATP
Electron Transport 34 ATP 38 ATPAnaerobic organisms can’t be too energetic but are important for global recycling of carbonSlide38Slide39Slide40