PDF-Proc. Okla. Acad. Sci. 79:85-86(1999)

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New Maximum Age of Bigmouth Buffalo Craig P Paukert

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Proc. Okla. Acad. Sci. 79:85-86(1999): Transcript

New Maximum Age of Bigmouth Buffalo Craig P Paukert. Proc.Natl.Acad.Sci.USA90(1993)8129Stimulusaz0C.)ww-JcnnNeuron'sResponseFourierFundamentalsAverage*Response=;;~~~~~~Noise=N-RiREALFIG.1.Calculationofaganglioncell'saverageresponseandresponsenoiseto10cy Proc.Natl.Acad.Sci.USA73(1976)29290.6OD0.50.41+mPMU0.3LDEPTH(mm)IIIIIIII00.51.0FIG.1.Atracedcomputeroutputdisplayingtheaveragedopticaldensitiesoflayersinthespecimenofcerebralcortexshownbelow.Thescanni MedicalSciences:Cherif-Zaharetal.Proc.Natl.Acad.Sci.USA87(1990)6245aatcccggcctgcacagagacggacacaggATGAGCTCTAAGTACCCGCGGTCTGTCCGG60MetSerSerLysTyrProArgSerValArg9CGCTGCCTGCCCCTCTGGGCCCTAACACTGGAAGCAGCTC Proc.Natl.Acad.Sci.USA93(1996)9293exonjunctionsinthespy-1andspy-2mutants,genomicDNAwassubjectedtoPCRandtheproductsweresequenceddirectly.RESULTSANDDISCUSSIONIsolationofspy-4.Thespy-4mutantwasisolatedby Proc.Natl.Acad.Sci.USA75(1978)RESULTSWhennormalhumanglobinDNAwasdigestedwithEcoRIandprobedwithamixtureofa-and(3-globincDNAs,fivemajorfragmentswereseen(Fig.1).Thelargestfragment,about21kilobases(kb)inl Proc.Natl.Acad.Sci.USA92(1995)inasignificantlyfasterreaction.Theformationrateofnativemoleculesonthisfastrefoldingchannelcorrespondstotherateexpectedforfoldingtooccurintheabsenceofpopulatedintermediate Proc.Natl.Acad.Sci.USA82(1985)855timeswiththesamebufferlackingNP-40andTritonX-100andwereresuspendedinthenick-translationbufferasde-scribedabovecontaining100,Ciof[3H]TTP(117Ci/mmol,NewEnglandNuclear)in Proc.Nat.Acad.Sci.USA70(1978)624~~~~~~~~~~~~24*0aS5!!402040608000020-tO60eo0as--40506~~~~~~~~~~~~~0GROWTHINTHEL00H000)CRW'0NTE0~Z04-20FIG.2.(A)Kineticsofinductionofthechloroplastisoleucyl-tRNAuponexpo 6148Biochemistry:LittleandHarperTable1.BacterialstrainsemployedSource,reference,StrainRelevantgenotypeorderivationAB1157recA+lexA+sup-37(15)DM49recA+lexA3sup-37(12)DM511recA+IexA3ts1-1sup-37(16)DM1187 I,fromFlavobacteriumokeanokoites,isamemberoftheunusual,typeIIsclassofrestrictionendonucleasesthatrec-ognizeaspecificDNAsequenceandcleavenonspecificallyashortdistanceawayfromthatsequence(1).Ibindstheco Proc.Natl.Acad.Sci.USA90(1993)pHP45QC(13)bySmaIdigestionandgelpurification.pIB415isaderivativeofpIB410(ofIncPreplicon)containingvirAA63/240,avirAderivativelacking178aminoacidsintheperiplasmicdomain(14 TheNunproteinofbacteriophageHK022isamemberofthearginine-richmotiffamilyofRNAbindingproteins,whichincludesthephageNtranscriptionantiterminationproteinandtheHIVTatandRevproteins(Fig.1;refs.1±3).Nunbind Cellsrespondtodevelopmentalcuesandenvironmentalstimulibyalteringgeneexpression.Often,transmissionofdifferentupstreamsignalsinvolvescommoncomponentsyetelicitsdistinct(butappropriate)outcomes.Numerousag Proc.Natl.Acad.Sci.USA82(1985)ofcellsoveriodinevapors.Cellsthataccumulateglycogenstainbrown,whereasthosethatdon'tremainyellow.Quan-titativetrehaloseandglycogendeterminationsweremadeaccordingtoLilliean

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