PPT-CRISPR/Cas9

Breakthrough in genome editing The most important scientific result of the year 2015 Science18 December 2015 I will report first about Duchenne muscular dystrophy because there were serious problems . DeMayo. FJ, Spencer TE. Jennifer Thornton. April 1, 2015. Happy April Fools’ Day!. *I won’t actually cover this paper, but it’s real and you should check it out at. http. ://www.ncbi.nlm.nih.gov/pubmed/25100711. Introduction to CRISPR. http://. www.addgene.org. /CRISPR/guide/. Wiedenheft. et al. Nature 2012. 3 distinct types of bacterial CRISPR systems identified so far:. Type I, II, III . Type II is the basis for current genome engineering applications. Presented By :. Amna. Muhammad. 09-Arid-1536. Ph. D Scholar . Biochemistry. 1. st. Semester. 12/16/2014. 1. Contents. History. Functional analysis of a gene. RNA . interfernce. . siRNA. shRNA. miRNA. The Wonderful World of CRISPR. As told by Professor Peter Shepherd. To do precise genetic engineering we need to be able to find and specifically modify regions of DNA. But the human genome has 3,000,000,000 base pairs so how are we going to find a 20 base pair region in this huge sea of DNA ? . Viruses. . Presented By: Diana Marquez. Introduction. The Rhinovirus, while often synonymous with the “common cold” has also been found to be a contributing factor for the occurrence of acute respiratory conditions such as asthma and inhibit effective infection resolution in at risk individuals. CRISPR Babies: Background and Ethics By: Austin Guse and Kaitlyn Curtis What is a CRISPR baby? An organism, specifically a human child, that has had its genetics altered in one way or another and has successfully been born or is still in guestation. Genomics. Lesson 12, presentation 3. Hardison. (several slides . from Dr. . Cooduvalli. . Shashikant. ). 4/26/15. 1. Genome Editing . Removal, modification, or addition of a functional element into the genome of a living cell or organism. A Neiteler. 1,2,3,5. , S Borooah. 6. , BT Selvaraj. 2,3,5. , K Burr. 2,3,5. , B Dhillon. 2,4. , JA Ross. 1. , S Chandran. 2,3,5. 1. Tissue Injury and Repair Group, . 2. Centre for Clinical Brain Sciences, . for creation of. p53 knock-outs in . human . glioma . cells. :. a. research proposal by . gus. . thomas. Overview and Introduction:. Glioblastoma Multiforme (GBM):. Aggressive brain cancer. Very poor prognosis. Modern methods in Molecular Biology. . Epigenetisc. Crispr. /CAs9. RNAi. Epigenetic  refers to heritable changes in gene expression (active versus inactive genes), that does not involve changes to the underlying DNA sequence; a change in phenotype without a change in genotype. . successfully applied to directly produce low-copy integrated transgenic lines in C. eleganss10]. High copy integrated arrays are prone to be silenced, yet low-copy transgenes permit relatively stable articleinfo2021TheAuthors.PublishedbyElsevierB.V.onbehalfofCairoUniversity.ThisisanopenaccessarticleundertheCCBY-NC-NDlicense(http://creativecommons.org/licenses/by-nc-nd/4.0/ Peerreviewunderresponsib Tocite:RedmanM,KingA,WatsonC,etalArchDisChildEducPractEdINTRODUCTIONClusteredregularlyinterspacedpalin-dromicrepeats(CRISPR)/Cas9isagene-editingtechnologycausingamajorupheavalinbiomedicalresearch.Itma DEFINITIONS Genome Editing : This is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or “molecular scissors”.