Viruses David Wick - PowerPoint Presentation

Viruses David Wick President and CEO 795 Porter Hill Rd., Stevensville, Montana 59870406-369-4214 mail mrwick@bvs-inc.us web – www.bvs-inc.us BVS, Inc.

What we have Virus detection using technology invented by the Army, the Integrated Virus Detection System - IVDSWhat we accomplished Virus identification by sizeWhat we are seeingSeasonal ChangesFrequency Changes Diversity ChangesViral count changes that can measure stress exposure and recoveryWhat we are doingVirus monitoring for beekeepersNational Honeybee Virus Mapping ProjectTreatments for Mites and Small Hive Beetles using virus BVS, Inc.

1 micron = 1000 nanometersNosema spores are about 15 X 25 microns Most bee Viruses are in the 17 to 46 nm size rangeIt takes more than just a microscope BVS, IncViruses are really small

IVDS Detecting Viruses Based on the physics of nanometer sized particle movement through a charged atmosphere Electrospray converts liquid sample to aerosol state and charges the particlesDifferential Mobility Analyzer collects and groups particles by size Condensation Particle Counter (CPC) counts particles in each size group BVS, Inc.

Honeybee Virus Mapping Project In this project, we are targeting hobby beekeepers. To look at bees that are stationary and do not move from crop-to-crop to answer the question: What are the regional differences in viruses across the U.S.?   To map the distribution, frequency, diversity and intensity of viral loads around the country.

Honeybee Testing Why Test?  

feeding,treatments, splits,requeening, buying bees, honey extraction and processing, labor, etc

feeding,treatments, splits,requeening, buying bees, honey extraction and processing, labor, etcYes, we do this so what?

Evaluation for each activity,

Evaluation for each activity, Based on what we know or have been taught

this is the way I was taught, this is the way my grandfather did it, this is the way that the USDA recommends, my friend does it this way and I will to because it worked for my friend, I went to the convention and this is what I took home with me, it sounds good, the national survey says…. , I read this study on this process, item, tool, feed, treatment, etc… .

Big or SmallWe all work our bees!

Big or SmallWe all work our bees!One way or another.

Factors of just looking and listening to your bees.

Frame strengthBrood coverageEgg layingFlightsThe surrounding area WaterFoodDo I have to supplement these?

These are just evaluations You are testing each condition to see if it is good or bad for your bees.

Test or evaluate for more detail

Nosema Mites Bacteria Hive beetles Wax moths Virus And others

We evaluate and test, To gain knowledge for our use and to share that gained knowledge with others .

The tools we use for evaluation come from many sources.

Tools for Evaluation Conventions and State MeetingsSpeakersTrade Show exhibits

Tools for Evaluation Websites and Online Publications

Tools for Evaluation USDA ARS Bee Labs Baton Rouge, LouisianaBeltsville, MarylandLogan, Utah Tucson, Arizona.

Project Apis mhttp://projectapism.org/ PAm is proud to support and sponsor research aimed at enhancing the health of honey bees. Results are published on their website.

Online resources as newslettersCatch the BuzzAPHA newsletter Google AlertsABFUniversities around the countryPenn State, and Montana State University Bee Informed Partnership

Randy OliverScientificBeekeeping.comBeekeeping Through the Eyes of a Biologist

Jerry J. BromenshenkUniversity of Montana Bee Alert Technology, Inc. Beeresearch@aol.com406 – 544 - 9007Retired, Not Expired!

  January 9 – February 17 February 27 – April 7 June 26 – August 4 October 30 - December 8 April 10 – June2 August 28 – October 20 June 12 – August 4 www.umt.edu/bee University Accredited, Beginner - Commercial

New Tools for Bee Management and Research Infrared for the Toolbox – 4 Articles are available online ( Bee Culture 2015-2016)Picking the right camera, how to use, pros and consQuestions, Tips, Uses (Online Public Forum , ~ March, 2017)

Acoustic Scanning: Detect Mites, Bee Diseases, Queen Loss, Pesticide Exposure iPhone and Android Apps for Smart Phones,Automatic Cloud Data Storage, Beta Testing by End of Month (Jan)

Satellite-Managed Asset TrackerMobile - Monitor Loads from Point of Origin to Delivery Fixed - Monitor Hive Pallets, Yards, Gates, Bear Traps 12 Reporting Time Options, Interval or Continuous MonitoringThree-Year Battery-Life, Built-In Motion Sensor, GPSCan Add-On Temperature Sensors, Scales, etc.Periodic and Emergency (e.g., Theft!!) Alerts by Text or Email2017, Newest Tracker TechnologySmallest (3¼” x 3¼” x 5/8”)Cheapest (~ $150-200 per tracker, depending on number units)Messaging as low as $14 month depending on frequency and amount of data. Available Now, Call Jerry, 406-544-9007

ExamplesGoogle Alerts Pesticides Cause Physiological Stress in Bees: Research French Tribune Stress Alone Can Lead to Bee Colony Collapse Discovery News The factors have little in common, except that they all stress the bees. A single factor in a low dose is not enough to kill them, but the stress alone is enough to make the bees exhibit erratic behavior — such as orientation, mobility, defense and other skills — as though the they are slightly boozed..

Google AlertsThe stress experienced by individual bees over a prolonged period of time begins to affect the entire colony. And at a critical level of stress, a tipping point, the colony heads toward failure, suggests a study published today in Ecology Letters. .

Google AlertsSwedish treatment helps ward off colony collapse disorder Fresh Fruit Portal A natural product combining pollen and lactic acid may help boost the defense of bees against colony collapse disorder (CCD), which has led to the destruction ... A Lund University research team has made an astounding discovery – bees have a battery of healthy bacteria in their honey stomach that protects them. Giving these lactic acid bacteria back to bees boosts their natural immune system, helping them fight off disease.

Google AlertsThis study shows a clear trend of rising viral titres in bee populations over the course of a season from spring to autumn. Despite the application of acaricide, varroa mites and viral titres continued to rise while remaining below the levels observed in the untreated group. In many cases, the treatment only helped to control varroa loads and viral titres to a limited extend, and led to no significant reduction in the prevalence of virus-sick bees. In part, this may be explained by the recent findings [47], [48], that show negative impact of acaricides on honey bee immunity. Adding to this effect of acaricides, the observed failure of the treatment to reduce varroa numbers in several colonies might have led to immuno-suppression syndrome as observed in other studies [18], [55].

Google Alertshttp://www.vita-europe.com/diseases/viruses/ Viruses are commonly present in any honeybee colony. However infection of honeybees and the appearance of disease symptoms seems to depend to a large extent on other stress factors on the colony, such as lack of space, food or water, weather or infection by other means be they bacterial, fungal or mite.

Google AlertsChemical treatment for colony collapse disorder temporarily worsens viral infections in honeybeesJan 20, 2012 Read more at: http://phys.org/news/2012-01-chemical-treatment-colony-collapse-disorder.html#jCp . “However, this research suggests that for maintaining low infestation, regular alternative control treatments, such as organic methods, could be used for promoting better bee health overall. Further, since the virus is not reduced in the colony as rapidly as the mites are removed, our research underlines the importance of early mite removal to reduce virus levels in overwintering bees, to avoid colony losses.”

Google AlertsResults of Research: Using Essential Oils for Honey Bee Mite ControlJim Amrine , Bob Noel, Harry Mallow, Terry Stasny, Robert Skidmore(Last Updated: December 30, 1996 ) James W. Amrine, Jr.Division of Plant and Soil Sciences, P. O. Box 6108, West Virginia University Morgantown, WV 26505-6108 USATelephone: 304-293-6023E-mail: jamrine@wvnvm.wvnet.edu We have found that several essential oils can either kill, or adversely affect varroa mites. Essential Oils have Two Modes of Action: 1) Toxicity by direct contact:When varroa mites contact essential oils such as wintergreen, patchouli, tea tree oil, et al., mixed into oil or grease, they are killed on contact--usually within a few minutes.

Google AlertsCanadian National NewspaperAccording to Dr. Michael Salla , there is a Military Extraterrestrial Industrial Complex or MIEC. Dr. John Lash suggests that lower dimensional entities rather than "Extraterrestrials" are piloting human vessels that are presiding over the destruction of our planet Earth. The ancient Pagan Gnostics referred to those lower dimensional entities as "Archons" [video below]. Does Dr. Lash's insights explain worsening negligence by elites on environmental issues?

Using these resources increase your knowledgeAll of these are resources that can be used for evaluation BVS, Inc.

some only require only reading, some require to change procedures, some require taking and sending samples , some require that you spend money to buy products or services, some require constant or regular application. BVS, Inc.

BVS, Inc is a tool for you to use, a gauge on your dash. It does cost money You have to collect bees and send them, you also have to read the report. BVS, Inc.

BVS, Inc.

BVS, Inc. 2009 Peak Detection Frequency 50%

BVS, Inc. 2009 Peak Detection Frequency 50% 15%

BVS, Inc. 2009 Peak Detection Frequency 50% 15% 17%

BVS, Inc. 2010 Peak Detection Frequency 2009 Peak Detection Frequency 46%

BVS, Inc. 2010 Peak Detection Frequency 2009 Peak Detection Frequency 46% 19%

BVS, Inc. 2010 Peak Detection Frequency 2009 Peak Detection Frequency 46% 19% 10%

BVS, Inc. Bees have viruses Virus infections can change over time

BVS, Inc. Bees have viruses Virus infections can change over timeVirus populations in the bees change with outside factors

BVS, Inc. Bees have viruses Virus infections can change over timeVirus populations in the bees change with outside factorsOutside factors can change viral lethalness

BVS, Inc. Bees have viruses Virus infections can change over timeVirus populations in the bees change with outside factorsOutside factors can change viral lethalness Viruses can exist within the bee with no symptoms

BVS, Inc. Do nothing Virus action points Sometimes this is the right answer.

BVS, Inc. Do nothing Treat with what and how? NutritionVirus action points

BVS, Inc. Do nothing Treat with what and how? Nutrition Essential oils Virus action points

BVS, Inc. Do nothing Treat with what and how? Nutrition Treat the mites Essential oils Virus action points

BVS, Inc. Do nothing Treat with what and how? Nutrition Essential oils Treat the mites Treat for Nosema Virus action points

BVS, Inc. Do nothing Treat with what and how?Use them as measurement tools Use regular virus monitoring as a tool for evaluating bee stress and reaction to outside factors.Virus action points

BVS, Inc. Do nothing Treat with what and how?Use them as measurement tools Monitor the viral loads for selective breeding Use virus screening as a tool for evaluating bee viral resistance or viral tolerance. Virus action points

BVS, Inc. Do nothing Treat with what and how?Use them as measurement tools Monitor the viral loads for selective breeding Understand what is affecting the bees Use virus monitoring as a tool for evaluating if there is a pesticide problem or a viral problem, mite influences Virus action points

Aggregate or single colony samples of bees from your apiary Track the viral load over the year for better results Send the bees alive !  That means to have the bees put into a quart sized zip lock baggie, alive, seal the baggie, put it into a flat rate box from the post office and mailed.  About 100 - 200 bees. BVS, Inc. Mail to: BVS, Inc. 795 Porter Hill Rd, Stevensville, Montana 59870 Sampling and Sending

The Lab BVS, Inc.

My Thanks To:California State Beekeepers AssociationProject Apis MAmerican Honey Producers AssociationAmerican Bee FederationState Beekeepers Associations Almond Board of CaliforniaMontana Department of AgricultureAnd Many othersBVS, Inc.

BVS, Inc. This is your lab, for your use, brought together by funding from Project Apis m, ABF, Associations and individuals.Your donations to research work and bring new tools to you. Thank you for supporting through your donations.

BVS, Inc. David Wick President and CEO 795 Porter Hill Rd., Stevensville, Montana 59870406-369-4214 email mrwick@montana.com Questions?

BVS, Inc. 2009 Peak Detection Frequency

BVS, Inc. 2009 Peak Detection Frequency

BVS, Inc. 2009 Peak Detection Frequency

BVS, Inc. 2009 Peak Detection Frequency

BVS, Inc. 2009 Peak Detection Frequency

BVS, Inc. 2009 Peak Detection Frequency

BVS, Inc. 2009 Peak Detection Frequency

BVS, Inc. 2009 Peak Detection Frequency